Leírás
Termékismertető megtekintése (angol)
This product is suitable for direct amplification of plant leaves, seeds, etc., and can be used for high-throughput screening of non-polysaccharide and polyphenol plant samples.The direct-amplifying DNA polymerase modified by directed evolution has strong tolerance to PCR inhibitors in plants, while maintaining high amplification performance, suitable for the amplification of DNA fragments within 5 kb; Plant Direct Lysis Buffer A can be used to lyse fresh or frozen plant tissue, and the resulting lysate can be used as an amplification template without purification.The protective agent added to the Lysis Buffer enables the crude product to be amplified effectively after multiple freeze-thaw cycles. The pre-prepared 2 × Plant Direct Master Mix only needs to add primers and templates to carry out amplification reactions, reducing pipetting operations and improving assay throughput and reproducibility of results.The amplified product is blunt-ended and is suitable for use with ClonExpress and TOPO cloning kits (C112/C113/C115/C601).
