Leírás
Termékismertető megtekintése (angol)
One Step Mouse Genotyping Kit is specially designed for the rapid genotyping of mouse, which contains a complete set of reagents for DNA extraction and PCR amplification. This kit can be used for rapid extraction of genomic DNA from mouse tails, ears, toes, and other tissues. The extracted genomic DNA can be used directly as template for PCR amplification with no need of homogenization, crushing, overnight digestion, phenol-chloroform extraction, DNA precipitation or column purification operations, which greatly shortens the experimental time. For using, the tissue should be soaked in lysis buffer with Proteinase K, incubated at 55°C for 20 min, then heat at 95°C for 5 min to inactivate the Proteinase K. After centrifugation, the obtained lysate can be used as PCR template directly. After repeated tests, it is widely applicable to the amplification of target fragments within 2 kb, and is suitable for multiple PCR reactions within 4 pairs of primers. This Kit is equipped with 2 × Taq Plus Master Mix (Dye Plus), which includes high-performance Taq Plus DNA Polymerase, dNTP, and optimized buffer system. Only need to add primers and template in PCR reaction to perform amplification, which reduces operations, significantly reduces sample cross-contamination and improves detection throughput and results reproducibility. The unique protective agent allows Taq Plus Master Mix to maintain stable activity after repeated freezing and thawing. The system is premixed with electrophoresis buffer and loading dye, and electrophoresis can be carried out directly after the reaction, which is convenient. The PCR product has A-tailing at the 3′ end, which can be cloned into T vector and is suitable for ClonExpress and topological cloning kit (Vazyme #C112/C113/C115/C601)
