This product is suitable for direct amplification of plant leaves, seeds, etc., and can be used for high-throughput screening of non-polysaccharide and polyphenol plant samples.The direct-amplifying DNA polymerase modified by directed evolution has strong tolerance to PCR inhibitors in plants, while maintaining high amplification performance, suitable for the amplification of DNA fragments within 5 kb; Plant Direct Lysis Buffer A can be used to lyse fresh or frozen plant tissue, and the resulting lysate can be used as an amplification template without purification.The protective agent added to the Lysis Buffer enables the crude product to be amplified effectively after multiple freeze-thaw cycles. The pre-prepared 2 × Plant Direct Master Mix only needs to add primers and templates to carry out amplification reactions, reducing pipetting operations and improving assay throughput and reproducibility of results.The amplified product is blunt-ended and is suitable for use with ClonExpress and TOPO cloning kits (C112/C113/C115/C601).

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Multiplex PCR refers to a PCR technology that simultaneously amplifies multiple gene loci in a single PCR reaction. The experiment processes are the same as conventional PCR, but multiple fragments can be detected simultaneously. It is of great significance in many fields such as scientific research and disease diagnosis, and is especially suitable for multi-site detection of trace samples. This kit contains Multiplex DNA Polymerase (multiplex PCR specific hot-start DNA polymerase), which has extremely high amplification specificity and sensitivity. Combined with 2 × Multiplex Buffer, a special buffer optimized for multiplex PCR reactions, this kit can stably and efficiently complete most multiplex PCR assays under default conditions. It has a wide range of applications and minimizes reaction system optimization steps.

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Taq Pro Multiplex DNA Polymerase is a new generation of hot-start DNA polymerase based on antibody modification and upgraded to improve template affinity. The High sensitivity version has been carefully optimized for multiplex PCR with strong amplification ability and high detection sensitivity. With the optimal buffer system for multiplex PCR, it is compatible with a wide range of product GC content and primer Tm values. The vast majority of multiplex PCR assays can be performed at a universal annealing temperature of 60°C without additional optimization.
This product has extremely high amplification efficiency, and can simultaneously amplify target fragments in the range of 50 – 3,500 bp; it has strong amplification ability, and can carry out enrichment of several hundred or more amplicons; excellent impurity tolerance, highly resistant to a variety of inhibitors and impurity types, and compatible with direct amplification of whole blood, blood cards, etc. The reagents have good stability, wide applicability, and are compatible with a variety of detection scenarios.

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Taq Pro Multiplex DNA Polymerase is a new generation of hot-start DNA polymerase based on antibody modification and upgraded to improve template affinity. The High specificity version has been carefully optimized for multiplex PCR with extremely high amplification specificity. With the optimal buffer system for multiplex PCR, it is compatible with a wide range of product GC content and primer Tm values, and most multiplex PCR assays can be completed at a universal annealing temperature of 60℃.
This product has extremely high amplification efficiency. All primers in the reaction system can be efficiently extended without additional optimization; the amplification preference is low, and the amplification efficiency is consistent for different types of primers and templates. Achieving high yields while maintaining good amplification uniformity; the amplification specificity is outstanding, which can effectively reduce the non-specific amplification caused by primer mismatch.

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Blood Direct PCR Kit can perform PCR directly of whole blood samples without the need for DNA purification or sample pretreatment. This kit can be used to perform PCR directly from a wide range of templates including fresh blood, blood stored at 4°C, frozen blood, and dried blood stored on Whatman 903 or FTA commercial card. It is compatible with all conventional anticoagulants (EDTA, citrate, heparin, etc.). The Kit contains the Phanta Blood Super-Fidelity DNA Polymerase, which is an engineering modification of the Phanta Blood Super-Fidelity DNA Polymerase. This enzyme displays superior resistance to the PCR inhibitors in the whole blood sample, and can amplify whole blood with concentration up to 40%. This kit can efficiently amplify genomic fragments of up to 10 kb from whole blood samples with the optimized 2 × Phanta Blood Buffer V2.Phanta Blood Super-Fidelity DNA Polymerase produces blunt-ended products, which are applicable to ClonExpress and TOPO cloning kits (Vazyme #C112/C113/C115/C601). This kit supplies Positive control primer mix that is compatible with mammalian and other vertebrate animals and can be used as positive control in experiments.

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The post 2 × Phanta Max Master Mix (Dye Plus) (P525) appeared first on SEQRS Kft..

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2 × Phanta Flash Master Mix is based on the new generation superior enzyme Phanta Flash Super-Fidelity DNA Polymerase. Through directed optimization of Phanta DNA Polymerase, Phanta Flash Super-Fidelity DNA Polymerase has the characteristics of rapid amplification (4 – 5 sec/kb) while maintaining high fidelity and yield. Matched with optimized buffer system, this kit can achieve high amplification specificity. And it has excellent compatibility with crude samples, templates with uracil and GC-rich system (primer/template). This kit contains two types of monoclonal antibodies inhibiting the 5’→3′ polymerase activity and 3’→5′ exonuclease activity at room temperature, which enable it to perform hot start PCR with great specificity. It contains all required reaction components (Phanta Flash Super-Fidelity DNA Polymerase, dNTP and optimized buffer), except primers and templates, thereby simplifying the operation process and improving the detection throughput and repeatability. Amplification will generate blunt-ended products, which are compatible with ClonExpress cloning kits (Vazyme #C112/C113/C115) and TOPO cloning kit (Vazyme #C601)

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Phanta Max Super-Fidelity DNA Polymerase is a new generation superior enzyme based on Phanta DNA Polymerase for robust PCR with higher fidelity. The unique extension factor, specificity-promoting factors and plateau un-inhibitory factor newly added to Phanta Max greatly improve its long-fragment amplification ability, specificity, and PCR yield. Phanta Max is capable of amplifying long fragments such as 40 kb λDNA, 40 kb plasmid DNA, 20 kb genomic DNA and 10 kb cDNA. The amplification error rate of Phanta Max is 53-fold lower than that of conventional Taq and 6-fold lower than that of Pfu. In addition, Phanta Max has a good resistance to PCR inhibitors and can be used for direct PCR amplifications of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. Phanta Max contains two monoclonal antibodies inhibit- ing the 5’→3′ polymerase activity and 3’→5′ exonuclease activity at room temperature, which enable Phanta Max to perform hot start PCR with great specificity. This kit contains Phanta Max Super-Fidelity DNA Polymerase, dNTP, and an optimized buffer system. It contains all required reaction components, except primers and templates, thereby simplifying the operation process and improving the detection throughput and repeatability. Protective agents in the 2 × Phanta Max Master Mix enable the resistance to repeated freezing and thawing. Amplification will generate blunt-ended products, which are compatible with ClonExpress and TOPO cloning kits (Vazyme #C112/C113/C115/C601).

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The post 2 × Phanta Max Master Mix (P515) appeared first on SEQRS Kft..

2 × Phanta Flash Master Mix is based on the new generation superior enzyme Phanta Flash Super-Fidelity DNA Polymerase. Through directed optimization of Phanta DNA Polymerase, Phanta Flash Super-Fidelity DNA Polymerase has the characteristics of rapid amplification (4 – 5 sec/kb) while maintaining high fidelity and yield. Matched with optimized buffer system, this kit can achieve high amplification specificity. And it has excellent compatibility with crude samples, templates with uracil and GC-rich system (primer/template). This kit contains two types of monoclonal antibodies inhibiting the 5’→3′ polymerase activity and 3’→5′ exonuclease activity at room temperature, which enable it to perform hot start PCR with great specificity. It contains all required reaction components (Phanta Flash Super-Fidelity DNA Polymerase, dNTP and optimized buffer), except primers and templates, thereby simplifying the operation process and improving the detection throughput and repeatability. This kit contains loading buffer, which can be directly spotted for electrophoresis after the reaction. Amplification will generate blunt-ended products, which are compatible with ClonExpress cloning kits (Vazyme #C112/C113/C115) and TOPO cloning kit (Vazyme #C601)

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The post 2 × Phanta Flash Master Mix (Dye Plus) (P520) appeared first on SEQRS Kft..