Leírás
Termékismertető megtekintése (angol)
Phanta Max Super-Fidelity DNA Polymerase is a new generation superior enzyme based on Phanta DNA Polymerase for robust PCR with higher fidelity. The unique extension factor, specificity-promoting factors and plateau un-inhibitory factor newly added to Phanta Max greatly improve its long-fragment amplification ability, specificity, and PCR yield. Phanta Max is capable of amplifying long fragments such as 40 kb λDNA, 40 kb plasmid DNA, 20 kb genomic DNA and10 kb cDNA. The amplification error rate of Phanta Max is 53-fold lower than that of conventional Taq and 6-fold lower than that of Pfu. In addition, Phanta Max has a good resistance to PCR inhibitors and can be used for direct PCR amplifications of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. Phanta Max contains two monoclonal antibodies inhibiting the 5’→3′ polymerase activity and 3’→5′ exonuclease activity at room temperature, which enable Phanta Max to perform hot start PCR with great specificity. Amplification will generate blunt-ended products, which are compatible with ClonExpress and TOPO cloning kits (Vazyme #C112/C113/C115/C601).
