This product is suitable for direct amplification of plant leaves, seeds, etc., and can be used for high-throughput screening of non-polysaccharide and polyphenol plant samples.The direct-amplifying DNA polymerase modified by directed evolution has strong tolerance to PCR inhibitors in plants, while maintaining high amplification performance, suitable for the amplification of DNA fragments within 5 kb; Plant Direct Lysis Buffer A can be used to lyse fresh or frozen plant tissue, and the resulting lysate can be used as an amplification template without purification.The protective agent added to the Lysis Buffer enables the crude product to be amplified effectively after multiple freeze-thaw cycles. The pre-prepared 2 × Plant Direct Master Mix only needs to add primers and templates to carry out amplification reactions, reducing pipetting operations and improving assay throughput and reproducibility of results.The amplified product is blunt-ended and is suitable for use with ClonExpress and TOPO cloning kits (C112/C113/C115/C601).

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Blood Direct PCR Kit can perform PCR directly of whole blood samples without the need for DNA purification or sample pretreatment. This kit can be used to perform PCR directly from a wide range of templates including fresh blood, blood stored at 4°C, frozen blood, and dried blood stored on Whatman 903 or FTA commercial card. It is compatible with all conventional anticoagulants (EDTA, citrate, heparin, etc.). The Kit contains the Phanta Blood Super-Fidelity DNA Polymerase, which is an engineering modification of the Phanta Blood Super-Fidelity DNA Polymerase. This enzyme displays superior resistance to the PCR inhibitors in the whole blood sample, and can amplify whole blood with concentration up to 40%. This kit can efficiently amplify genomic fragments of up to 10 kb from whole blood samples with the optimized 2 × Phanta Blood Buffer V2.Phanta Blood Super-Fidelity DNA Polymerase produces blunt-ended products, which are applicable to ClonExpress and TOPO cloning kits (Vazyme #C112/C113/C115/C601). This kit supplies Positive control primer mix that is compatible with mammalian and other vertebrate animals and can be used as positive control in experiments.

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One Step Mouse Genotyping Kit is specially designed for the rapid genotyping of mouse, which contains a complete set of reagents for DNA extraction and PCR amplification. This kit can be used for rapid extraction of genomic DNA from mouse tails, ears, toes, and other tissues. The extracted genomic DNA can be used directly as template for PCR amplification with no need of homogenization, crushing, overnight digestion, phenol-chloroform extraction, DNA precipitation or column purification operations, which greatly shortens the experimental time. For using, the tissue should be soaked in lysis buffer with Proteinase K, incubated at 55°C for 20 min, then heat at 95°C for 5 min to inactivate the Proteinase K. After centrifugation, the obtained lysate can be used as PCR template directly. After repeated tests, it is widely applicable to the amplification of target fragments within 2 kb, and is suitable for multiple PCR reactions within 4 pairs of primers. This Kit is equipped with 2 × Taq Plus Master Mix (Dye Plus), which includes high-performance Taq Plus DNA Polymerase, dNTP, and optimized buffer system. Only need to add primers and template in PCR reaction to perform amplification, which reduces operations, significantly reduces sample cross-contamination and improves detection throughput and results reproducibility. The unique protective agent allows Taq Plus Master Mix to maintain stable activity after repeated freezing and thawing. The system is premixed with electrophoresis buffer and loading dye, and electrophoresis can be carried out directly after the reaction, which is convenient. The PCR product has A-tailing at the 3′ end, which can be cloned into T vector and is suitable for ClonExpress and topological cloning kit (Vazyme #C112/C113/C115/C601)

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This kit contains a complete set of crude DNA extraction from animal tissues and QPCR amplification system with anti-pollution probe method, which is suitable for rapid genotyping of mice (Rapid Genotyping). This kit can be used to quickly release genomic DNA from mouse tails, ears, toes and other tissues. The products are directly amplified by QPCR, avoiding operations such as multiple open capping and pipetting. The genotype results are directly obtained by software analysis, avoiding manual checking The input introduces errors and can greatly reduce the time-consuming experiment. The dUTP/UDG anti-pollution system in the reagent can rapidly degrade U-containing pollutants at room temperature, significantly reducing aerosol pollution and improving the detection throughput and reproducibility of results.

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