RNA-easy Isolation Reagent is widely applicable to extraction of Total RNA and Small RNA from various animal tissues, plant materials, cultured cells, bacteria and other samples. Compared with the traditional Trizol extraction method, this product is simple to use, eliminating the need to use chloroform for phase separation, and the whole process can be carried out at room temperature. In addition, the product effectively inhibits RNase activity, while simultaneously precipitating impurities such as protein, DNA and polysaccharides to the bottom of the tube, so as to enable single-phase extraction and powerfully guarantee the integrity and purity of the RNA. The whole extraction process can be completed within 50 min with this product. The extracted RNA can be directly used in various molecular biology experiments such as cDNA cloning, qRT-PCR, mRNA purification, in vitro translation, Northern blotting hybridization, high-throughput sequencing and so on.

The post RNA-easy Isolation Reagent (R701) appeared first on SEQRS Kft..

This kit is a simple and rapid reagent for blood lysis at room temperature. This kit contains Lysis Buffer and Stabilizing Buffer. Lysis Buffer contains special components that rapidly destroy cell membrane proteins and membrane structure, which can fully release genomic DNA in cells. Stabilizing Buffer contains protective proteins and stabilizing factors, which can eliminate inhibitory effect of inhibitory factory in the sample on the downstream qPCR and PCR reactions, so that the DNA solution can be stored for a long time.

Vazyme oldal megtekintése (angol)

The post RoomTemp Sample Lysis Kit (P073) appeared first on SEQRS Kft..

This kit is suitable for extracting genomic DNA from ≤200 μl of fresh or frozen anticoagulated whole blood, <25 mg of animal tissue, <5 × 106 of cultured cells and <3 × 109 cultured bacterial samples. The kit is based on silica gel column purification technology that eliminates the need for extraction using phenol/chloroform organic solvents or time-consuming alcohol precipitation. With this kit, RNA, proteins, lipids and other inhibitory impurities can be removed at the greatest extent. The DNA obtained can be directly used in PCR, qPCR, restriction enzyme digestion and virus detection

Vazyme oldal megtekintése (angol)

The post FastPure Blood / Cell / Tissue / Bacteria DNA Isolation Mini Kit (DC112) appeared first on SEQRS Kft..

FastPure Blood DNA Isolation Mini Kit V2 is specially designed for rapid and efficient purification of genomic DNA from 0.1 ml – 1 ml of fresh and frozen whole blood with common anticoagulants. The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for toxic phenol-chloroform extractions, or time-consuming ethanol precipitation. RNA, proteins, Lipids and other impurities such as enzyme inhibitors are removed effectively by the wash buffer. Isolated DNA with high purity and stable quality can be used directly in down-steam applications including restriction enzyme digestion, PCR, Southern blotting.

The post FastPure Blood DNA Isolation Mini Kit V2 (DC111) appeared first on SEQRS Kft..

This kit can quickly extract total RNA from animal tissues and cells. The kit is based on silica gel column purification technology. There is no need to use toxic phenol/chloroform extraction or β-mercaptoethanol to extract high-quality RNA during the extraction process. The whole process only takes 6 minutes. The FastPure gDNA-Filter Columns Ⅲ in the kit can effectively remove impurities and gDNA, and FastPure RNA Columns Ⅲ can efficiently bind RNA. With optimized buffer, the total RNA obtained is high in purity, less gDNA residues, and free of protein and other impurities. It can be used for various downstream experiments such as RT-PCR, Real-Time PCR, chip analysis, etc.

Vazyme oldal megtekintése (angol)

The post FastPure Cell/Tissue Total RNA Isolation Kit V2 (RC112) appeared first on SEQRS Kft..

MiPure Cell/Tissue miRNA Kit is suitable for the extraction of small RNAs (miRNA, <200 nt) from fewer than 5 × 106 eukaryotic cells or less than 100 mg of animal tissues. The obtained miRNAs are of high purity without large RNAs or genomic DNA contamination, which can be directly used for chip analysis, Northern hybridization, RT-PCR and miRNA library preparation, etc. Based on a combination of high performance RNA Isolater extraction reagent and silica gel column purification technique, the extraction of miRNAs can be completed within 1 h with this kit. This kit can also be used to separate and extract large RNAs (>200 nt) or total RNA (including miRNAs) to meet different needs.

Vazyme oldal megtekintése (angol)

The post MiPure Cell/Tissue miRNA Kit (RC201) appeared first on SEQRS Kft..

The Vazyme RNA Isolater Total RNA Extraction Reagent uses guanidinium isothiocyanate and phenol, with high lysis ability. It can lyse cells and tissues in short time, keeping integrity of RNA. The RNA isolater Total RNA Extraction Reagent is widely used for RNA extraction of cultured cells, animal tissues, microorganisms, and plant tissues with weak secondary metabolism (i.e. seedling and spire). Samples will be lysed thoroughly in RNA isolater and form supernatant, interlayer, and organic layer (bright red sublayer). RNA is distributed in the upper water phase. Remove the RNA-containing supernatant layer and treat with isopropanol to extract the total RNA.

The post RNA isolater Total RNA Extraction Reagent (R401-01) appeared first on SEQRS Kft..

The Bacteria RNA Extraction Kit is a kit optimized for RNA extraction from prokaryotes, including Gram-negative and Gram-positive bacteria. The kit includes a bacterial RNA Plus Reagent containing an optimized buffer system, a chelating agent and a mild protein denaturant, and an RNA isolater Total RNA Extraction Reagent that efficiently inhibits endogenous RNase and promotes protein denaturation. The combination of the two can greatly improve the extraction yield of prokaryotic RNA while ensuring good product quality. When extracting bacterial RNA, a high-temperature pretreatment is added only before the routine operation, and no high-quality and high-yield bacterial total RNA can be obtained without mechanical disruption or enzymatic hydrolysis steps. This kit is also suitable for the extraction of total RNA from fungi such as yeast.

The post Bacterial RNA Extraction Kit (R403-01) appeared first on SEQRS Kft..

This kit is suitable for extracting plasmid from 150-300 mL of the bacterial solution cultured overnight , using an improved SDS-alkaline lysis method to lyse the bacteria. The crude extract is selectively combined with a unique Endotoxin Scavenger and separated by centrifugation to remove endotoxins. Then, teh silicon matrix membrane in the centrifugal adsorption colun selectively binds to plasmid DNA in the solution under conditions of high salt and low pH. This is followed by addition of wash buffer to remove impurities and other becterial components. Finally, a low-salt, high-pH elution buffer is used to elute pure plasmid DNA from the silicon matrix membrane. The silicon matrix membrane in centrifugal adsorption column employs imported special adsorption membrane, and the adsorption amount difference between the column and the column is very small and the repeatability is well. Phneol, chloroform and other toxic reagents are not required, and neither is ethanol required for precipitation. This kit can be used to rapidly extract 0.2-1.5 mg of pure high-copy plasmid DNA, with an extraction rate of 80% – 90%. The unique process formula removes endotoxin, the content of endotoxin is extremely low and the cell transfection effect is excellent. The extracted plasmid could be directly used in enzymatic digestion, PCR, in vitro transcription, transformation, sequencing and other molecular biology experiments.

Vazyme oldal megtekintése (angol)

The post FastPure EndoFree Plasmid Maxi Kit (DC202-01) appeared first on SEQRS Kft..

This Kit based on optimized SDS alkaline lysis technology, is applicable for the purification of plasmids DNA from 1 – 5 ml overnight cultures. By using this kit, the extraction of multiple samples can be finished rapidly within 30 min without phenol chloroform or ethanol precipitation. The unique Filtration Column contained in the kit bind the plasmid DNA specifically under high-salt and low-pH condition, maximally removing proteins, genomic DNA, RNA and other impurities. Then the bound plasmid DNA is eluted from silicon membrane in a low-salt and high-pH buffer (Each column can absorb maximally 35 ug of plasmid DNA). The purified plasmid DNA can be directly used for traditional biological experiments: enzyme digestion, PCR, sequencing, ligation, transformation, and transfection of sub-culture cells.

The post FastPure Plasmid Mini Kit (DC201-01) appeared first on SEQRS Kft..