Leírás
Termékismertető megtekintése (angol)
This Kit based on optimized SDS alkaline lysis technology, is applicable for the purification of plasmids DNA from 1 – 5 ml overnight cultures. By using this kit, the extraction of multiple samples can be finished rapidly within 30 min without phenol chloroform or ethanol precipitation. The unique Filtration Column contained in the kit bind the plasmid DNA specifically under high-salt and low-pH condition, maximally removing proteins, genomic DNA, RNA and other impurities. Then the bound plasmid DNA is eluted from silicon membrane in a low-salt and high-pH buffer (Each column can absorb maximally 35 ug of plasmid DNA). The purified plasmid DNA can be directly used for traditional biological experiments: enzyme digestion, PCR, sequencing, ligation, transformation, and transfection of sub-culture cells.
