Termékismertető megtekintése (angol)
The VAHTS Universal DNA Library Prep Kit for Ion Torrent V2 is a DNA library preparation kit optimized specifically for the Ion Torrent high-throughput sequencing platform. This kit can convert 5 ng to 1 ug of input DNA into a dedicated library for the Ion Torrent high-throughput sequencing platform. As a new upgrade version, the VAHTS Universal DNA Library Prep Kit for Ion Torrent V2 greatly increases the library conversion rate and amplified library yield through overall improvement of the end repair module, the ligation module and the library amplification module. This kit is widely applicable to PCR or PCR-free library preparation of various samples, and is compatible with the target capture process. All the reagents provided in the kit have undergone rigorous quality control and functional test to ensure the optimal stability and repeatability of library preparation.
Termékismertető megtekintése (angol)
VAHTS Universal Plus DNA Library Prep Kit for Illumina V2 is a DNA library preparation kit featuring enzyme-based fragmentation specifically developed for Illumina high-throughput sequencing platforms. This kit is an upgraded version of the original one. It significantly reduces the proportion of Artificial Invert Chimera Reads in the DNA library of FFPE samples and improves the reliability of the detection of SNV and other biomarkers while maintaining the original high performance. The kit combines DNA fragmentation, end repair, and dA-tailing into one step. The adapter ligation, library amplification, and size selection can be performed directly without additional purification. The 100 pg to 1 μg template DNA can be converted into the library suitable for Illumina high-throughput sequencing platforms. The kit is perfectly compatible with DNA from different sources and ofdifferent input amounts, and the user only needs to adjust the fragmentation time based on the target insert size to obtain a library with the desired fragment size.
VAHTS ssDNA Library Prep Kit for Illumina is a single-stranded DNA library preparation kit targeted at Illumina high-throughput sequencing platform. Based on efficient single-strand ligation technology, this kit can convert single DNA into sequencable libraries. It is also suitable for double-stranded DNA library preparation, as well as single-and double-stranded mixed DNA library preparation. It is compatible with DNA samples as short as 40 bp and 10 pg-250 ng Input DNA. It is suitable for severely damaged, denatured or degraded DNA samples, and is an ideal choice for the preparation of difficult and precious samples. The optimized database building process can be completed within 2 h, and the manual operation time is less than 30 min. It is suitable for automatic database building and compatible with capture process. All reagents in the kit underwent strict quality control and functional verification, which ensured the stability and repeatability of library preparation to the greatest extent.
Termékismertető megtekintése (angol)
VAHTS Universal Plus DNA Library Prep Kit for Illumina® is specially designed for DNA library preparation for next generation sequencing (NGS) on Illumina® platforms. This kit combines DNA fragmentation, end repair and dA tailing into one step. The products can be directly used to adapter Ligation without purification, then for library enrichment and size selection. This kit is suitable for library preparation from 100 pg – 1 μg of input DNA. No mechanical fragmentation of the genome simplifies the process of library construction and shortens the operation time, suitable for the construction of PCR and PCR-Free libraries. This kit is perfectly compatible with DNA from different sources and different inputs, and the required fragment size library can be obtained by adjusting the fragmentation time according to the size of the target insert. All kit components are subjected to stringent functional quality control, ensuring the consistency and reproducibility of library preparation.
VAHTS Universal DNA Library Prep Kit for Illumina V4 is a library preparation kit targeted for Illumina high-throughput sequencing platform optimization. 100 pg-1 μg of fragmented double-stranded DNA can be converted into a dedicated library for Illumina high-throughput sequencing platform. As a new generation of upgraded version, the kit can improve the conversion rate of low-quality template libraries and reduce the repetition rate of libraries by optimizing and improving the terminal repair module, connecting module and library amplification module. This kit is widely suitable for PCR or PCR-free library preparation of a variety of samples, and is compatible with the targeted capture process. All reagents provided in the kit have undergone strict quality control and functional validation to ensure the stability and repeatability of library preparation to the greatest extent.
Termékismertető megtekintése (angol)
VAHTS Universal Pro DNA Library Prep Kit for Illumina is specially designed for library preparation for next generation sequencing (NGS) on Illumina® platforms, containing FFPE DNA repair modules, suitable for library preparation from 100 pg – 1 μg of input DNA. This kit contains a DNA damage repair module that can effectively repair DNA damage caused by formalin-fixed paraffin-embedded (FFPE), such as deamination of cytosine, nicks and gaps, oxidized bases, blocked 3´ ends, etc, compatible with common DNA samples without affecting the quality of normal DNA sample libraries. Through the overall improvement of the module of end-repair, ligation and library amplification, the library conversion rate and the amplification output are greatly improved. It is widely applicable to PCR or PCR-Free library construction of multiple samples, and is compatible with targeted capture process.
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VAHTSTM Universal DNA Library Prep Kit for Illumina® V3 is a library construction kit optimized for the Illumina® high-throughput sequencing platform. This kit can convert 100 pg-4 μg Input DNA into a dedicated library for the Illumina® high-throughput sequencing platform. As a new upgraded version, VAHTSTM Universal DNA Library Prep Kit for Illumina® V3 greatly improves the library conversion rate and amplified library output through the overall improvement of the end repair module, the connection module and the library amplification module. PCR or PCR-Free library construction of various samples, and compatible with the targeted capture process. All reagents provided in the kit have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.
VAHTS Target Capture Universal Blockers and Post-PCR Primer Mix for MGI-SI contains Universal Blockers for MGI-SI and Post-PCR Primer Mix for MGI-SI contains two components. Among them, Universal Blockers for MGI-SI is a universal splice blocking sequence directed at MGI platform DNA library. This universal Blockers is suitable for single-ended Barcode labeled DNA library. It can effectively reduce the series structure formed between different library molecules due to the annealing between joints during hybridization, so as to reduce the non-specific hybridization and improve the medium target rate of hybridization capture reaction. Post-PCR Primer Mix for MGI-SI is a universal amplification primer targeted to single-ended barcode-labeled DNA libraries on the MGI platform. All components in the kit have gone through strict quality control and functional verification, which ensures the stability and repeatability of the experiment to a large extent.
VAHTS Target Capture Hybridization and Wash Kit is a complementary kit developed specifically for the Vazyme targeted capture hybridization and Wash series. Carefully optimized hybrid reaction system and capture cleaning system ensure the stability and uniformity of capture efficiency. All reagents provided in the kit have undergone strict quality control and functional verification to ensure the stability and repeatability of the experiments to the greatest extent.
VAHTS Target Capture Universal Blockers and Post-PCR Primer Mix for Illumina-TS contains Universal Blockers for IlluminA-TS Illumina-TS and Post-PCR Primer Mix for Illumina-TS two fractions. Among them, Universal Blockers for IlluminA-TS is a generic adaptor blocking sequence directed at Illumina common DNA libraries. This universal Blockers is suitable for DNA libraries labeled with single or double ends of 6-8 bp Barcode. It can effectively reduce the series structure formed between different library molecules due to the annealing between joints during hybridization, so as to reduce the non-specific hybridization and improve the medium target rate of hybridization capture reaction. Post-PCR Primer Mix for IlluminA-TS is a universal amplification primer directed to Illumina’s TruSeq DNA library. All components in the kit have gone through strict quality control and functional verification, which ensures the stability and repeatability of the experiment to a large extent.
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VAHTS AmpSeq Multi-PCR Module V3 is an amplification reagent based on ultra-multiplex PCR technique to construct amplicon libraries. The initial template for this reagent is 1 – 100 ng. It is compatible with genomic DNA, FFPE samples, cfDNA, etc. This optimized reagent contains enzymes, dNTPs and reaction buffer required during multiplex PCR process, reducing the pipetting operations, making the result more stable. Compared to previous generation (Vazyme #NA205), this reagent has better amplification performance for different input amounts and customized panels. The upgrade ensures the high coverage and high uniformity of the amplicon library. This reagent is compatible with up to 7000 multiplex customized panels. This reagent is suitable for the library preparation for amplicon sequencing, helping researchers and testing personnel to complete ultra-multiplex PCR quickly and easily with high sequencing quality. The reagent has undergone rigorous quality control and functional testing, to ensure the optimal stability and repeatability of library preparation.
Termékismertető megtekintése (angol)
VAHTS® RNA Multi-PCR Library Prep Kit is based on multiplex PCR and can be used for RNA sequencing library preparation. This kit contains reverse transcription module and multi-PCR based library preparation module. Two rounds of PCR amplification are adopted to complete target amplification and enrichment. It only takes 5 h from viral genomic sample acquisition to obtaining complete sequencing library, and the manual operation time is less than 1.5 h. This product is compatible with the starting template range of 16 CT – 38 CT, and the constructed library is suitable for sequencing analysis on the Illumina platform. The customized panel in this kit has a coverage of 99.7% of the whole genome. With multi-PCR amplification reagents, the uniformity can reach 90%, which can effectively detect the mutation and deletion sites. The optimized combination of the reagents enables easy, stable and reliable RNA library construction. All reagents provided in the kit have undergone strict quality control and functional verification, ensuring the optimal stability and repeatability of library construction.
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VAHTS AmpSeq Library Prep Kit V2 is based on ultra-multiplex PCR, introducing several core technologies such as end primer digestion, to construct amplicon libraries. The entire library construction workflow of this kit takes place in a single tube, without intermediate purification steps. It takes as little as 5 h from amplification to the generated library, including less than 1.5 h of manual operation. The initial template for this kit is 1 – 100 ng. It is compatible with genomic DNA, FFPE samples, cfDNA, etc. This kit is intended for both the Illumina and Ion Torrent high-throughput sequencing platforms. And corresponding adapters (Vazyme #NA111/NA121) are also provided. It is compatible with panels including Ion AmpliSeq Series Panel and AmpliSeq for Illumina Series Panel, as well as the corresponding individually customized panels. Compared to the previous generation (Vazyme #NA101), this kit greatly improves the compatibility of samples such as FFPE and cfDNA, while ensuring the high coverage and high uniformity of the amplicon library. The optimized multiplex amplification mix reduces pipetting operations. The results of library construction are more stable and reliable, helping researchers and testing personnel to complete high quality amplicon library construction quickly and easily. All the reagents provided in the kit have undergone rigorous quality control and functional testing, to ensure the optimal stability and repeatability of library construction.
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VAHTS Serum/Plasma Circulating DNA Kit is based on the purification technology of superparamagnetism beads. This kit is designed for extracting high quality cell-free DNA (cfDNA) from 200 µl-2 ml of serum, plasma, and other cell-free liquid samples. The kit has been optimized for low molecular weight nucleic acids, with high extraction efficiency, purity, and reproducibility. The obtained cfDNA is stable and can be directly used for qPCR and NGS library construction. The kit is compatible with high-throughput instruments of liquid-transfer nucleic acid extraction, further reducing laboratory labor and possible manipulation errors in experiments.
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AHTS RNAAdapters Set 1-Set 2 for Illumina is a special library preparation kit for Illumina high-throughput sequencing platform. It is suitable for multi-sample RNA library preparation on Illumina high-throughput sequencing platform. The Set 1 kit (Vazyme #N803) contains RNA Adapter numbers 1-12 for a total of 12 different indexes; The Set 2 kit (Vazyme #N804) contains adapters for RNA Adapter numbers 13-27 for a total of 12 different indices. All reagents provided in the kit have undergone strict quality control and functional validation to ensure the stability and repeatability of library preparation to the greatest extent.
Termékismertető megtekintése (angol)
AHTS RNAAdapters Set 1-Set 2 for Illumina is a special library preparation kit for Illumina high-throughput sequencing platform. It is suitable for multi-sample RNA library preparation on Illumina high-throughput sequencing platform. The Set 1 kit (Vazyme #N803) contains RNA Adapter numbers 1-12 for a total of 12 different indexes; The Set 2 kit (Vazyme #N804) contains adapters for RNA Adapter numbers 13-27 for a total of 12 different indices. All reagents provided in the kit have undergone strict quality control and functional validation to ensure the stability and repeatability of library preparation to the greatest extent.
Termékismertető megtekintése (angol)
VAHTS DNA Adapters Set 1/2 for Illumina are specially designed for DNA library preparation for Illumina platforms. The set 1 (Cat.No. #N801) contains 12 kinds of indexed adapters (DNA Adapter 1 – 12). The set 2 (Cat.No. #N802) contains another 12 kinds of indexed adapters (DNA Adapter 13 – 27).
