Leírás
Termékismertető megtekintése (angol)
VAHTS AmpSeq Library Prep Kit V2 is based on ultra-multiplex PCR, introducing several core technologies such as end primer digestion, to construct amplicon libraries. The entire library construction workflow of this kit takes place in a single tube, without intermediate purification steps. It takes as little as 5 h from amplification to the generated library, including less than 1.5 h of manual operation. The initial template for this kit is 1 – 100 ng. It is compatible with genomic DNA, FFPE samples, cfDNA, etc. This kit is intended for both the Illumina and Ion Torrent high-throughput sequencing platforms. And corresponding adapters (Vazyme #NA111/NA121) are also provided. It is compatible with panels including Ion AmpliSeq Series Panel and AmpliSeq for Illumina Series Panel, as well as the corresponding individually customized panels. Compared to the previous generation (Vazyme #NA101), this kit greatly improves the compatibility of samples such as FFPE and cfDNA, while ensuring the high coverage and high uniformity of the amplicon library. The optimized multiplex amplification mix reduces pipetting operations. The results of library construction are more stable and reliable, helping researchers and testing personnel to complete high quality amplicon library construction quickly and easily. All the reagents provided in the kit have undergone rigorous quality control and functional testing, to ensure the optimal stability and repeatability of library construction.
