Szállítási osztály: Normál

2 × Phanta Flash Master Mix (Dye Plus) (P520)
Termékismertető megtekintése (angol)
2 × Phanta Flash Master Mix is based on the new generation superior enzyme Phanta Flash Super-Fidelity DNA Polymerase. Through directed optimization of Phanta DNA Polymerase, Phanta Flash Super-Fidelity DNA Polymerase has the characteristics of rapid amplification (4 – 5 sec/kb) while maintaining high fidelity and yield. Matched with optimized buffer system, this kit can achieve high amplification specificity. And it has excellent compatibility with crude samples, templates with uracil and GC-rich system (primer/template). This kit contains two types of monoclonal antibodies inhibiting the 5’→3′ polymerase activity and 3’→5′ exonuclease activity at room temperature, which enable it to perform hot start PCR with great specificity. It contains all required reaction components (Phanta Flash Super-Fidelity DNA Polymerase, dNTP and optimized buffer), except primers and templates, thereby simplifying the operation process and improving the detection throughput and repeatability. This kit contains loading buffer, which can be directly spotted for electrophoresis after the reaction. Amplification will generate blunt-ended products, which are compatible with ClonExpress cloning kits (Vazyme #C112/C113/C115) and TOPO cloning kit (Vazyme #C601)
Vazyme oldal megtekintése (angol)
december 2, 2022
2 × Phanta Max Master Mix (P515)
Termékismertető megtekintése (angol)
Phanta Max Super-Fidelity DNA Polymerase is a new generation superior enzyme based on Phanta DNA Polymerase for robust PCR with higher fidelity. The unique extension factor, specificity-promoting factors and plateau un-inhibitory factor newly added to Phanta Max greatly improve its long-fragment amplification ability, specificity, and PCR yield. Phanta Max is capable of amplifying long fragments such as 40 kb λDNA, 40 kb plasmid DNA, 20 kb genomic DNA and 10 kb cDNA. The amplification error rate of Phanta Max is 53-fold lower than that of conventional Taq and 6-fold lower than that of Pfu. In addition, Phanta Max has a good resistance to PCR inhibitors and can be used for direct PCR amplifications of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. Phanta Max contains two monoclonal antibodies inhibit- ing the 5’→3′ polymerase activity and 3’→5′ exonuclease activity at room temperature, which enable Phanta Max to perform hot start PCR with great specificity. This kit contains Phanta Max Super-Fidelity DNA Polymerase, dNTP, and an optimized buffer system. It contains all required reaction components, except primers and templates, thereby simplifying the operation process and improving the detection throughput and repeatability. Protective agents in the 2 × Phanta Max Master Mix enable the resistance to repeated freezing and thawing. Amplification will generate blunt-ended products, which are compatible with ClonExpress and TOPO cloning kits (Vazyme #C112/C113/C115/C601).
Vazyme oldal megtekintése (angol)
december 2, 2022
2 × Phanta Flash Master Mix (P510)
Termékismertető megtekintése (angol)
2 × Phanta Flash Master Mix is based on the new generation superior enzyme Phanta Flash Super-Fidelity DNA Polymerase. Through directed optimization of Phanta DNA Polymerase, Phanta Flash Super-Fidelity DNA Polymerase has the characteristics of rapid amplification (4 – 5 sec/kb) while maintaining high fidelity and yield. Matched with optimized buffer system, this kit can achieve high amplification specificity. And it has excellent compatibility with crude samples, templates with uracil and GC-rich system (primer/template). This kit contains two types of monoclonal antibodies inhibiting the 5’→3′ polymerase activity and 3’→5′ exonuclease activity at room temperature, which enable it to perform hot start PCR with great specificity. It contains all required reaction components (Phanta Flash Super-Fidelity DNA Polymerase, dNTP and optimized buffer), except primers and templates, thereby simplifying the operation process and improving the detection throughput and repeatability. Amplification will generate blunt-ended products, which are compatible with ClonExpress cloning kits (Vazyme #C112/C113/C115) and TOPO cloning kit (Vazyme #C601)
Vazyme oldal megtekintése (angol)
december 2, 2022
Phanta UC Super-Fidelity DNA Polymerase for Library Amplification (P507)
All components stored at -20°C
Vazyme oldal megtekintése (angol)
december 2, 2022
Phanta Max Super-Fidelity DNA Polymerase (P505d)
Termékismertető megtekintése (angol)
Phanta Max Super-Fidelity DNA Polymerase is a new generation superior enzyme based on Phanta DNA Polymerase for robust PCR with higher fidelity. The unique extension factor, specificity-promoting factors and plateau un-inhibitory factor newly added to Phanta Max greatly improve its long-fragment amplification ability, specificity, and PCR yield. Phanta Max is capable of amplifying long fragments such as 40 kb λDNA, 40 kb plasmid DNA, 20 kb genomic DNA and10 kb cDNA. The amplification error rate of Phanta Max is 53-fold lower than that of conventional Taq and 6-fold lower than that of Pfu. In addition, Phanta Max has a good resistance to PCR inhibitors and can be used for direct PCR amplifications of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. Phanta Max contains two monoclonal antibodies inhibiting the 5’→3′ polymerase activity and 3’→5′ exonuclease activity at room temperature, which enable Phanta Max to perform hot start PCR with great specificity. Amplification will generate blunt-ended products, which are compatible with ClonExpress and TOPO cloning kits (Vazyme #C112/C113/C115/C601).
Vazyme oldal megtekintése (angol)
december 2, 2022
2 × AceTaq Master Mix (Dye Plus) (P412)
Termékismertető megtekintése (angol)
This product includes AceTaq® DNA Polymerse, dNTPs, and an optimized buffer system that allows for amplification by simply adding primers and templates, reducing pipetting and improving throughput and reproducibility. A protective agent added to the amplification system allows the 2 × AceTaq® Master Mix to maintain stable activity after repeated freeze-thaw cycles. This product is available in a version containing electrophoresis buffer and dye. It can be directly electrophoresed after the reaction. The 3′ end of the PCR product, A, can be directly cloned into the T vector and used in the ClonExpress® Cloning Kit (C112/C113/C114).
december 2, 2022
2 × AceTaq Master Mix (P411)
Termékismertető megtekintése (angol)
This product includes AceTaq® DNA Polymerse, dNTPs, and an optimized buffer system that allows for amplification by simply adding primers and templates, reducing pipetting and improving throughput and reproducibility. A protective agent added to the amplification system allows the 2 × AceTaq® Master Mix to maintain stable activity after repeated freeze-thaw cycles. This product is available in a version containing electrophoresis buffer and dye. It can be directly electrophoresed after the reaction. The 3′ end of the PCR product, A, can be directly cloned into the T vector and used in the ClonExpress® Cloning Kit (C112/C113/C114).
december 2, 2022
AceTaq DNA Polymerase (P401)
Termékismertető megtekintése (angol)
AceTaq DNA Polymerase is a chemically modified Taq DNA Polymerase that is completely blocked at room temperature and is released only after heating at 95℃. Non-specific amplification and primer dimerization can be prevented during sample preparation and temperature rise. Compared with the antibody-based hot-start Taq, the polymerase activity of AceTaq DNA Polymerase is blocked more stringently and completely. It takes only 5 min to activate AceTaq DNA Polymerase. AceTaq DNA Polymerase is compatible with most existing PCR protocols. Combined with an optimized buffer system, AceTaq DNA Polymerase minimizes non-specific amplification and primer dimers, ensuring extremely high sensitivity and specificity, which make it ideal for amplifying low-copy genes from complex templates. The PCR products contain A at the 3′ end and can be directly cloned into T-Vector. The products are compatible with ClonExpress and Top Cloning kits (Vazyme #C112/C113/C115/C601).
december 2, 2022
2 × Vazyme LAmp Master Mix (Dye Plus) (P312)
Termékismertető megtekintése (angol)
2× Vazyme LAmp Master Mix is a blend of Taq DNA Polymerase and a DNA proofreading polymerase with 3’ to 5’ exonuclease activity. Its fidelity was 6-fold higher than conventional Taq DNA Polymerase. Used with the optimized buffer system, 2× Vazyme LAmp Master Mix is applicable to long PCR products, up to 21 kb. This Master Mix is also able to amplify long fragments accurately from templates of different sources or different length. 2× Vazyme LAmp Master Mix contains Vazyme LAmp DNA Polymerase, dNTP, and optimized buffer. The reaction can be started by adding only primers and template, which simplifies the operation, improves through-put, and enhances result reproducibility. The protective agents included guarantees the stability of the activity of this Master Mix. The PCR product, containing dA at 3′-end, can be cloned into T-vector, and is suitable for One Step Express cloning kit (C112/C113).
december 2, 2022
2 × Vazyme LAmp Master Mix (P311)
Termékismertető megtekintése (angol)
2× Vazyme LAmp Master Mix is a blend of Taq DNA Polymerase and a DNA proofreading polymerase with 3’ to 5’ exonuclease activity. Its fidelity was 6-fold higher than conventional Taq DNA Polymerase. Used with the optimized buffer system, 2× Vazyme LAmp Master Mix is applicable to long PCR products, up to 21 kb. This Master Mix is also able to amplify long fragments accurately from templates of different sources or different length. 2× Vazyme LAmp Master Mix contains Vazyme LAmp DNA Polymerase, dNTP, and optimized buffer. The reaction can be started by adding only primers and template, which simplifies the operation, improves through-put, and enhances result reproducibility. The protective agents included guarantees the stability of the activity of this Master Mix. The PCR product, containing dA at 3′-end, can be cloned into T-vector, and is suitable for One Step Express cloning kit (C112/C113).
december 2, 2022
Vazyme LAmp DNA Polymerase (Mg2+ free buffer, with dNTP) (P302d)
Vazyme LAmp DNA Polymerase is a mixed enzyme composed of Taq DNA Polymerase and protein with 3’→5′ exonuclease activity (proofreading activity), and its fidelity is 6 times that of Taq DNA Polymerase. With specially optimized buffer system, Vazyme LAmp DNA Polymerase is very suitable for the amplification of long fragments. It can amplify fragments up to 21 kb from the genome, and has high amplification efficiency for templates of different sources and different lengths. PCR products have A-tailing at the 3′ end, which can be cloned into the T vector, and are suitable for ClonExpress and TOPO cloning kits (Vazyme #C112/C113/C115/C601). The attached PCR Enhancer facilitates the amplification of fragments with high GC content.
december 2, 2022
Vazyme LAmp DNA Polymerase (Mg2+ plus buffer, with dNTP) (P301d)
Vazyme LAmp DNA Polymerase is a mixed enzyme composed of Taq DNA Polymerase and protein with 3’→5′ exonuclease activity (proofreading activity), and its fidelity is 6 times that of Taq DNA Polymerase. With specially optimized buffer system, Vazyme LAmp DNA Polymerase is very suitable for the amplification of long fragments. It can amplify fragments up to 21 kb from the genome, and has high amplification efficiency for templates of different sources and different lengths. PCR products have A-tailing at the 3′ end, which can be cloned into the T vector, and are suitable for ClonExpress and TOPO cloning kits (Vazyme #C112/C113/C115/C601). The attached PCR Enhancer facilitates the amplification of fragments with high GC content.
december 2, 2022
Vazyme LAmp DNA Polymerase (Mg2+ plus buffer) (P301)
Termékismertető megtekintése (angol)
Vazyme LAmp DNA Polymerase is a mixed enzyme composed of Taq DNA Polymerase and protein with 3’→5′ exonuclease activity (proofreading activity), and its fidelity is 6 times that of Taq DNA Polymerase. With specially optimized buffer system, Vazyme LAmp DNA Polymerase is very suitable for the amplification of long fragments. It can amplify fragments up to 21 kb from the genome, and has high amplification efficiency for templates of different sources and different lengths. PCR products have A-tailing at the 3′ end, which can be cloned into the T vector, and are suitable for ClonExpress and TOPO cloning kits (Vazyme #C112/C113/C115/C601). The attached PCR Enhancer facilitates the amplification of fragments with high GC content.
december 2, 2022
2 × Rapid Taq Master Mix (P222)
Termékismertető megtekintése (angol)
2 × Rapid Taq Master Mix contains Taq DNA Polymerase, elongation promoting factor, dNTP and an optimized buffer system. The amplification speed of this product can reach 15 sec/kb, which is suitable for rapid PCR. The maximum amplification speed within 1 kb can reach 1 sec/kb, greatly saving reaction time. The pre-prepared 2 × Master Mix only needs to add primers and templates to perform amplification, which reduces pipetting operations and improves detection throughput and results reproducibility. The kit has excellent amplification performance and high storage stability. It is suitable for PCR amplification within 5 kb using genome as template and PCR amplification within 10 kb using plasmid and λDNA as template. The protective agent added to the system allows 2 × Master Mix to maintain stable activity after repeated freezing and thawing. The kit provides a version containing electrophoresis buffer and green loading dye, which can be directly electrophoresed after the reaction that is convenient to use. The PCR product has A-tailing at the 3′ end, which can be directly cloned into T vector and is suitable for ClonExpress and TOPO cloning kits (Vazyme #C112/C113/C115/C601).
Vazyme oldal megtekintése (angol)
december 2, 2022
2 × Taq Plus Master Mix II (Dye Plus) (P213)
Termékismertető megtekintése (angol)
This product contains Taq Plus DNA Polymerase, dNTP and an optimized buffer system. It can be amplified by adding primers and templates, which reduces pipetting operations and improves throughput and results reproducibility. The protective agent added to the system allows Master Mix to maintain stable activity after repeated freezing and thawing. This product is available in versions containing running buffers and loading dye for direct electrophoresis after the reaction. The PCR product has an A at the 3’ end, which can be cloned into the T vector and is suitable for ClonExpress rapid cloning kit (C112/C113).
Vazyme oldal megtekintése (angol)
december 2, 2022
2 × Taq Plus Master Mix (Dye Plus) (P212)
Termékismertető megtekintése (angol)
2 × Taq Plus Master Mix contains Taq Plus DNA Polymerase, dNTP and an optimized buffer system. Compared with Taq DNA Polymerase, it has higher fidelity, stronger amplification performance and yield. It can be used for PCR amplification within 10 kb using genome as template and PCR amplification within 15 kb using plasmid and λDNA as template. The pre-prepared 2 × Master Mix only needs to add primers and templates to perform amplification when used in PCR reactions, which reduces pipetting operations and improves detection throughput and results reproducibility. The protective agent added to the system allows 2 × Master Mix to maintain stable activity after repeated freezing and thawing. The kit provides a version containing electrophoresis buffer and dyes, which can be directly electrophoresed after the reaction that is convenient to use. The PCR product has A-tailing at the 3′ end, which can be cloned into T vector and is suitable for ClonExpress and TOPO cloning kits (Vazyme #C112/C113/C115/C601).
Vazyme oldal megtekintése (angol)
december 2, 2022
2 × Taq Plus Master Mix (P211)
Termékismertető megtekintése (angol)
This product contains Taq Plus DNA Polymerase, dNTP and an optimized buffer system. It can be amplified by adding primers and templates, which reduces pipetting operations and improves throughput and results reproducibility. The protective agent added to the system allows Master Mix to maintain stable activity after repeated freezing and thawing. This product provides a version containing electrophoresis buffer and dyes, which can be directly electrophoresed after the reaction, which is convenient to use. The PCR product has an A at the 3’ end, which can be cloned into the T vector and is suitable for ClonExpress® rapid cloning kit (C112/C113).
Vazyme oldal megtekintése (angol)
december 2, 2022
Taq Plus DNA Polymerase (P201)
Termékismertető megtekintése (angol)
Taq Plus DNA polymerase is a mixture of Taq DNA polymerase and an enzyme containing 3’→5’ exonuclease activity. The fidelity of Taq Plus is 6 times greater than that of Taq DNA Polymerase. Compared with Taq DNA Polymerase, Taq Plus DNA polymerase has stronger amplification performance, higher sensitivity and yield, and is more tolerant of impurities within 5 kb amplifying range. The obtained PCR products are compatible with ClonExpress II One Step Cloning Kit series (Vazyme, #C112, #C113). The PCR products contain A at the 3’-end and can be directly cloned into T-Vectors.
december 2, 2022
Taq HS DNA Polymerase (P132)
Termékismertető megtekintése (angol)
Taq HS DNA polymerase is a hot-start Taq polymerase obtained by mixing Champagne Taq antibody with Taq DNA polymerase in an optimal ratio. Due to the unique thermo stability of Champagne Taq antibody, the activity of Taq HS DNA polymerase is still blocked at temperature up to 55℃, which minimizes non-specific amplification during the mixing and system heating. When the reaction is kept at 95℃ for more than 30 sec, Champagene Taq antibody is completely inactivated and Taq enzyme activity is completely released, ensuring that the PCR system has extremely high amplification sensitivity and specificity. The activation of Taq HS DNA polymerase is not affected by pH, ionic strength, etc. It is applicable for various hot-start PCR and qPCR based on Taq DNA polymerase and can be used to amplify gene with low copy numbers from complex templates (genome and cDNA). It is the hot-start Taq enzyme of choice for PCR/qPCR molecular diagnostic reagents. This product has higher stability and detection rate.
Vazyme oldal megtekintése (angol)
december 2, 2022
Green Taq Mix (P131)
Termékismertető megtekintése (angol)
This product is a 2 × Master Mix that contains Taq DNA Polymerase, dNTPs and an optimized buffer system. It only needs to add primers and templates to perform the reaction, reducing errors caused by pipetting operations, and improving throughput and results. The optimized buffer system can effectively inhibit non-specific amplification and the generation of primer dimers. The protective agent added to the system allows Mix to maintain stable activity after repeated freezing and thawing. This product contains green loading dye and can be directly electrophoresed after the reaction. The PCR product has an A at the 3’ end, which can be cloned into the T vector and is suitable for ClonExpress rapid cloning kit (C112/C113/C115/C601).
Vazyme oldal megtekintése (angol)
december 2, 2022