Hyperactive pA-Tn5 Transposase for CUT&Tag is a fusion of Protein G and engineering modified ultra-high activity Tn5 transposase to form both transposase and a novel fusion enzyme for Protein G activity, specially adapted to the CUT&Tag technique for protein-genome interaction studies. Compared with ChIP-Seq, a traditional method for studying protein-genome interactions,CUT&Tag has significant advantages, such as short experimental period, high signal-to-noise ratio, good reproducibility, and low cell input volume, especially for early embryonic development, stem cells, tumors, and tableResearch fields such as outlook genetics.
Hyperactive pG-Tn5 Transposase for CUT&Tag is a fusion of Protein G and engineering modified ultra-high activity Tn5 transposase to form both transposase and a novel fusion enzyme for Protein G activity, specially adapted to the CUT&Tag technique for protein-genome interaction studies. Compared with ChIP-Seq, a traditional method for studying protein-genome interactions,CUT&Tag has significant advantages, such as short experimental period, high signal-to-noise ratio, good reproducibility, and low cell input volume, especially for early embryonic development, stem cells, tumors, and tableResearch fields such as outlook genetics.
Termékismertető megtekintése (angol)
RNA-easy Isolation Reagent is widely applicable to extraction of Total RNA and Small RNA from various animal tissues, plant materials, cultured cells, bacteria and other samples. Compared with the traditional Trizol extraction method, this product is simple to use, eliminating the need to use chloroform for phase separation, and the whole process can be carried out at room temperature. In addition, the product effectively inhibits RNase activity, while simultaneously precipitating impurities such as protein, DNA and polysaccharides to the bottom of the tube, so as to enable single-phase extraction and powerfully guarantee the integrity and purity of the RNA. The whole extraction process can be completed within 50 min with this product. The extracted RNA can be directly used in various molecular biology experiments such as cDNA cloning, qRT-PCR, mRNA purification, in vitro translation, Northern blotting hybridization, high-throughput sequencing and so on.
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HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) is an upgraded version of HiScript II 1st Strand cDNA Synthesis Kit (+gDNA wiper). It includes a new generation of reverse transcriptase HiScript III Reverse Transcriptase and optimized Buffer further improves the efficiency of one-strand synthesis. The 5 × gDNA wiper Mix in this kit can quickly remove genomic DNA contamination at 42°C for 2 min, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need of spanning exon junction to design primers. The kit contains single-component reverse transcription primers Oligo (dT)20VN and Random hexamers. Users can flexibly choose reverse transcription primers for subsequent experiments according to their needs. This kit can synthesize full-length cDNA (up to 20 kb) for downstream experiments such as cloning, and can also synthesize highly uniform cDNA for qPCR .
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Murine RNase Inhibitor is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form. It can extensively inhibit various RNases (RNase A, B, C). Murine RNase Inhibitor has undergone RT-PCR and RT-qPCR tests and is compatible with HiScript II Reverse Transcriptase, HiScript Reverse Transcriptase, M-MLV (RNaseH-) Reverse Transcriptase and various DNA Polymerases. Compared with human-derived RNase inhibitor, mouse-derived RNase inhibitor does not contain two cysteines that are very sensitive to oxidation in human protein, so it has higher antioxidant activity and is more suitable for experiments sensitive to high DTT (Such as qPCR).
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HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Compared with the previous generation of HiScript® Reverse Transcriptase, HiScript® II has further greatly improved thermal stability and is very suitable for reverse transcription of RNA templates with complex secondary structures. In addition, HiScript® II adds multiple point mutations, which further enhances template affinity and progress, and has higher tolerance to common reverse transcription inhibitors, which greatly improves the ability to synthesize full-length cDNA. HiScript® II 1st Strand cDNA Synthesis Kit is a first-strand cDNA synthesis kit based on HiScript® II Reverse Transcriptase. It contains all the components needed to synthesize high-quality first-strand cDNA. The product is suitable for subsequent PCR, qPCR and other experiments. 2 × RT Mix contains optimized buffer system and dNTP; HiScript® II Enzyme Mix contains HiScript® II Reverse Transcriptase and RNase inhibitor. The Oligo (dT)23VN included in the kit has a stronger anchoring ability for Poly A+ mRNA than Oligo (dT)18, making reverse transcription more efficient. Users can choose Oligo (dT)23VN, Random hexamers or gene-specific primers as reverse transcription primers according to their needs. The subsequent flexible and optional operation steps can not only synthesize full-length cDNA (up to 20 kb) for cloning, but also efficiently synthesize cDNA with uniform reverse transcription efficiency for each position of qPCR.
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HiScript II Reverse Transcriptase is a new generation reverse transcriptase optimized from the M-MLV (RNase H-) Reverse Transcriptase. Compared with the last generation reverse transcriptase, the thermo-stability of this product is significantly improved. The half-life of HiScript II Reverse Transcriptase at 50℃ is >240 min. At 55℃, it can also be stable for a long time, which significantly benifits the transcription of RNA templates with complex secondary structure. In addition, it has a improved template affinity and cDNA synthesis efficiency. It has a good resistance to most RT-PCR inhibitors and is suitable for long-fragment cDNA amplification (as long as 20 kb).
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Animal Detection Probe Super Premix is a specialized master mix for probe qPCR to detect African swine fever virus (ASFV). Only need to add extra primers, probes and templates. This master mix utilizes an upgraded hot start Taq polymerase with a carefully optimized buffer to increase detection sensitivity for low-concentration templates, while supporting the primer probe to be premixed in advance. It is also compatible with fast program, reducing test time.
Animal Detection Probe Master Mix is a specialized master mix for probe qPCR to detect African swine fever virus (ASFV). Only need to add extra primers, probes and templates. This master mix utilizes an upgraded hot start Taq polymerase with a carefully optimized buffer to increase detection sensitivity for low-concentration templates. It is also compatible with fast program, reducing test time.
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Taq Pro Multiple Probe qPCR Mix is a master mix for probe qPCR to detect DNA templates (such as DNA viruses). The core component, Taq Pro HS DNA Polymerase, is a new generation hot start DNA polymerase that has been modified based on antibody technique and upgraded to improve template affinity. Equipped with the most suitable buffer optimized for the qPCR system, amplification performance with multiple targets, amplification specificity and sensitivity for detection of low-copy genes and amplification curve shape are significantly improved. It can provide excellent amplification curve within a wide quantitative range, and accurately quantify and detect target genes, with good repeatability and high reliability. It has wide compatibility in terms of template type, template GC content, and primer Tm values. And it also has good tolerance to impurities and is suitable for use in a variety of testing scenarios. The product is a 2 × master mix. Only primers, probes and templates need to be added additionally. It is convenient to use and compatible with fast program to reduce test time.
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Taq Pro HighGC Multiple Probe qPCR Mix is a master mix for probe qPCR to detect DNA templates (such as DNA viruses). The core component, Taq Pro HS DNA Polymerase, is a new generation hot-start DNA polymerase that has been modified based on antibody techniques and upgraded to improve template affinity. Equipped with the most suitable Buffer optimized for the qPCR system, amplification specificity and sensitivity for detection of low-copy genes are significantly improved. It can provide excellent amplification curve within a wide quantitative range, and accurately quantify and detect target genes, with good repeatability and high reliability. This product is suitable for the amplification of rich GC templates and has good impurity tolerance. The reagent incorporates an anti-contamination system, which works at room temperature to eliminate the effect of amplification product contamination on qPCR to ensure the accuracy of the results. The product is a 2 × master mix. Only primers, probes and templates need to be added additionally. It is convenient to use and compatible with fast program to reduce test time.
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ChamQ Geno-SNP Probe Master Mix is specially designed for single nucleotide polymorphism (SNP) typing by probe-based qPCR. Only primers, probes, and templates need to be added additionally, which is convenient. Champagne Taq DNA Polymerase is the core component of this master mix. Matched with optimized buffer system, the success rate of SNP typing from low-concentration and complex templates is increased. The reagent incorporates an anti-contamination system, which works at room temperature to eliminate the amplification products contamination to ensure the accuracy of SNP typing. This product contains a unique ROX Passive Reference Dye that is suitable for all qPCR instruments. The concentration of ROX does not need to be adjusted on different instruments.
Termékismertető megtekintése (angol)
This product is a special premix for qPCR reaction using SYBR Green I chimeric fluorescence method. The core component, Taq Pro DNA Polymerase, is a new generation of hot-start polymerase modified by antibody method. It has many advantages such as strong specificity, high detection sensitivity and high amplification yield. With the combination of Buffer optimized for qPCR and specific enhancer, it is very suitable for qPCR reaction with high specificity and sensitivity. This kit contains Specific ROX Reference Dye, which is suitable for all qPCR instruments without adjusting the concentration of ROX on different instruments. This kit contains 2 × master mix that can be amplified by adding primers and template.
Termékismertető megtekintése (angol)
This product is a special premix for qPCR reaction using SYBR Green I chimeric fluorescence method. The core component Champagne Taq DNA Polymerase is a new type of antibody-based hot-start DNA polymerase, which has many advantages such as strong specificity and high detection sensitivity. It is equipped with the most suitable buffer optimized for qPCR and the patented specific promoter Exactor, which is very suitable for high specificity and high sensitivity qPCR. This product contains a special ROX Passive Reference Dye, which is suitable for all qPCR instruments, so that no need to adjust the concentration of ROX on different instruments, only need to add primers and templates when preparing the reaction system to perform amplification.
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AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE) is a one-tube probe-based RT-qPCR master mix suitable for single or multiplex quantitative PCR detection using RNA as template (such as RNA virus). The enzyme in this master mix uses a hot-start technology based on enzyme modification, with a new generation of high-affinity Taq enzyme antibodies, to achieve efficient blocking and precise release.
Combined with one-step reverse transcriptase and optimized chemical buffer, it supports pre-mixing of primers and probes in advance and the mix can be stored for a long time at low temperature. The sample to be tested can be added directly when using, no additional tube opening/pipetting operation. The anti-contamination system in this product can function at room temperature to eliminate the influence of other contamination on qPCR and ensure the accuracy of the results. This product is also compatible with fast programs, which greatly shortens the detection time.
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HiScript III One Step qRT-PCR Probe 5 × Master Mix is specially designed for single-plex or multiplex qPCR detection using RNA (e.g. virus RNA) as template. It has extremely high stability, which can be stored at 37℃ for 14 days and its performance remains unchanged, and remains liquid form at a storage temperature of – 20℃ to avoid the thawing process of reagents. The reaction can be started by directly adding the template, primers and target probes required for detection. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. It can be inactivated at 55℃ without affecting the efficiency and sensitivity of qRT-PCR. Combining the superior performance of HiScript III Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase, with an optimized buffering system, the detection sensitivity of HiScript III One Step qRT-PCR Probe 5 × Master Mix can reach 0.1 pg of total RNA or less than 10 copies of RNA templates and is suitable for high-specificity detection systems based on fluorescence labelled probes (e.g. TaqMan).
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AceQ Universal Probe Master Mix V2 adopts rigorous chemical modification Acetaq, with a carefully optimized buffer system, which can greatly improve the sensitivity of the probe method. At the same time, AceQ Universal Probe Master Mix V2 also uses a special ROX Passive Reference Dye, which is suitable for all qPCR instruments without the need to adjust the ROX concentration and is easy to use.
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AceQ Universal SYBR qPCR Master Mix is a special premix for qPCR reaction using SYBR Green I chimeric fluorescence method. The core component, AceTaq DNA Polymerase, is a chemically modified hot-start DNA polymerase. Combined with the optimal Buffer optimized for qPCR, the AceTaq DNA Polymerase can effectively inhibit non-specific amplification, thus significantly improving the amplification efficiency, and it is suitable for high sensitivity qPCR reaction. This product is a 2 × premix SYBR Green I premix containing the optimal concentration for qPCR reaction. It can obtain a good standard curve in a wide quantitative range, and accurately quantify and detect target genes with good repeatability and high reliability. This kit contains special ROX, Passive Reference Dye, which is suitable for all qPCR instruments. There is no need to adjust the concentration of ROX on different instruments, and the amplification can be performed with the addition of primers and templates when preparing the reaction system.
