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AceQ Universal Probe Master Mix V2 adopts rigorous chemical modification Acetaq, with a carefully optimized buffer system, which can greatly improve the sensitivity of the probe method. At the same time, AceQ Universal Probe Master Mix V2 also uses a special ROX Passive Reference Dye, which is suitable for all qPCR instruments without the need to adjust the ROX concentration and is easy to use.
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HiScript II One Step qRT-PCR Probe Kit (Clean) is specially designed for qPCR detection using RNA (e.g., RNA virus) as templates. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube. No additional opening/pipetting operations are required, greatly increasing assay throughput and reducing the risk of contamination. Combining the superior performance of HiScript II Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase, equipping with an optimized buffer, the detection sensitivity of HiScript II One Step qRT-PCR Probe Kit (Clean) can reach 0.1 pg of total RNA or <10 copies of RNA templates. This product is provided as master mix. The 2 × One Step Mix contains an optimized buffer and dNTP/dUTP mix, and is suitable for high-specificity detection systems based on fluorescence labelled probes (e.g., TaqMan).
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AceTaq® DNA Polymerase is a chemically modified Taq DNA Polymerase whose activity is completely blocked at room temperature to prevent non-specific amplification and primer dimers during sample preparation and reaction temperature rise. The activity is released only after heating at 95°C. Compared with antibody-based hot-start Taq enzymes, AceTaq® DNA Polymerase has a more complete and stricter activity closure; compared with existing chemically modified hot-start Taq enzymes, the activation time of AceTaq® DNA Polymerase only takes 5 minutes. Compatible with existing PCR programs. AceTaq® DNA Polymerase combined with an optimized buffer system can minimize non-specific amplification and primer dimers, bring the highest sensitivity, and is very suitable for amplifying low-copy genes from complex templates (genome, cDNA). The PCR product has an A at the 3’ end, which can be cloned into the T vector and is suitable for ClonExpress® Rapid Cloning Kit (C112-C113).
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The ExFect2000 Transfection Reagent is a novel and efficient transfection reagent based on cationic liposomes, which is suitable for DNA transfection and co-transfection systems for most eukaryotic cells (both adherent and suspended). The ExFect2000 has a unique structure and an optimized formulation. The presence of serum and antibiotics will not affect the efficiency of transfection, thereby reducing the effect of serum-deprival on cells. The ExFect2000 is also with low cytotoxicity. After transfection, there’s no need to remove nucleic acidExFect2000 complex or replace medium within 24 hr-48 hr. The high transfection efficiency of ExFect2000 transfection reagent has been validated in a board range of eukaryotic cell lines.
The VAHTS Target Capture Core Exome Panel is designed with Imwaldt probes and consists of over 400,000 120 nt ssDNA probes. Human GRC h38/hg38 was used as the reference genome, covering 19,441 genes in Refseq, CCDS, ClinVar and other databases, and the target capture region was 33.9 Mb, which was compatible with mixed capture of up to 12 samples.
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VAHTS AmpSeq Library Prep Kit V3 is based on ultra-multiplex PCR, introducing several core technologies such as end primer digestion, to construct amplicon libraries. The entire library preparation workflow of this kit takes place in a single tube, the sequencing library can be obtained after purification. It takes as little as 5 h from amplification to the generated library, including less than 1.5 h of manual operation. The initial template for this kit is 1 – 100 ng. It is compatible with genomic DNA, FFPE samples, cfDNA, etc. This kit is intended for both the Illumina and Ion Torrent high-throughput sequencing platforms. And corresponding adapters (Vazyme #NA111/ NA121) are also provided. It is compatible with panels including Ion AmpliSeq Series Panel and AmpliSeq for Illumina Series Panel, as well as the corresponding individually customized panels. Compared to the previous generation (Vazyme #NA201), the library preparation and sequencing quality have been greatly improved. The optimized multiplex amplification module has good amplification performance for different input amounts and customized panels. The upgraded digestion module can effectively solve the problem of PCR products contamination and improve data utilization. The newest ligation module increases the effective library ratio. Comprehensive product upgrade ensures the high coverage and high uniformity of the amplicon library. With this kit, the sequencing results are more stable and reliable, helping researchers and testing personnel to complete high quality library preparation quickly and easily. All the reagents provided in the kit have undergone rigorous quality control and functional testing, to ensure the optimal stability and repeatability of library preparation.
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VAHTSTM RNA Clean Beads is based on the principle of (Solid Phase Reverse Immobilization), which can effectively remove protein, salt ions and other impurities, and is suitable for RNA purification. VAHTSTM RNA Clean Beads and the widely used AGENCOURT RNACLEANXP are used in exactly the same way. They can seamlessly replace AGENCOURT RNACLEANXP, effectively reducing your experimental cost.
This product is the third generation TOPO cloning kit, which contains Topoisomerase, vector and self-ligation inhibitor. The kit uses a new type of Topoisomerase, with the optimal buffer, the enzyme activity is higher, and the high-efficiency ligation can be completed in 5 minutes. The addition of a vector self-ligation inhibitor increases the positive rate of clones. This product is suitable for blunt-end cloning.
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ClonExpress Ultra One Step Cloning Kit is a new generation of recombinant cloning kits, compatible with 1 – 5 fragments homologous recombination. The unique ligase-independent system of this kit significantly reduces the self-ligation background of the vector. Restriction endonuclease sites do not need to be considered. Highly optimized 2 × ClonExpress Mix (It contains enhanced recombinase Exnase) can significantly improve the recombination efficiency and the tolerance to impurities. pCE-Zero vector is compatible with most PCR products, enabling the specific PCR products to be used directly for recombination without any treatments, which significantly simplify the procedure.
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T7 RNA polymerase can perform in vitro transcription of RNA from DNA templates containing T7 promoter using four NTPs as substrates. T7 RNAi Transcription Kit is an optimized version of T7 High Yield RNA Transcription Kit that is designed for transcription of double-stranded RNA. It can be used to transcribe 21 bp siRNA and long-fragment dsRNA. The purified transcripts can be used for RNAi experiments mediated by cationic liposome, calcium phosphate coprecipitation, electroporation, DEAE-Dextran and microinjection. In general, one reaction produces 20 – 80 μg RNA.
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The T7 High Yield Transcription Kit is an optimized system for high yield in vitro transcription of RNA from DNA templates containing T7 RNA Polymerase promoter. The kit contains T7 RNA Polymerase which can synthesize RNA quickly and easily from the downstream of the T7 promoter, and obtains a large amount of RNA. Modified nucleotide can be added to the system to generate biotin or dye-labeled RNA.This kit can yield 150 – 200 μg of RNA with a template input of 0.5 μg. The RNA yield can be widely used in many downstream applications such as RNA structure and function studies, RNase protection, probe hybridization, RNAi, microinjection and in vitro translation.
TruePrep Index Kit for MGI is a special Kit for TruePrep DNA Library Prep Kit for MGI, which is specially suitable for library preparation on BTU high-throughput sequencing platform. The kit contains 24 NTMXXX and can be used for 24 different single-ended Index library preparations.
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Hyperactive In-Situ ChIP Library Prep Kit for Illumina is specifically designed for Cleavage Under Targets and Tagmentation (CUT&Tag) technology. CUT&Tag technology is a new method for research on protein-genomic interaction by fusion of Protein G or Protein A with engineered ultra – active Tn5 transposase to form a novel dual-function fusion enzyme (Hyperactive pG- Tn5 / pA-Tn5 Transposase). It precisely binds the DNA sequence near the target protein under the antibody guidance and results in factor-targeted tagmentation, generating fragments used for PCR enrichment or DNA sequencing. Compared with the traditional protein-genomic interaction research method of ChIP-Seq, CUT&Tag has significant advantages of low cell input, short operation time, high signal-to-noise ratio, good repeatability and is especially suitable for research on early embryo development, stem cells, tumors, and epigenetics.
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Hyperactive In-Situ ChIP Library Prep Kit for Illumina is specifically designed for Cleavage Under Targets and Tagmentation (CUT&Tag) technology. CUT&Tag technology is a new method for research on protein-genomic interaction by fusion of Protein G or Protein A with engineered ultra – active Tn5 transposase to form a novel dual-function fusion enzyme (Hyperactive pG- Tn5 / pA-Tn5 Transposase). It precisely binds the DNA sequence near the target protein under the antibody guidance and results in factor-targeted tagmentation, generating fragments used for PCR enrichment or DNA sequencing. Compared with the traditional protein-genomic interaction research method of ChIP-Seq, CUT&Tag has significant advantages of low cell input, short operation time, high signal-to-noise ratio, good repeatability and is especially suitable for research on early embryo development, stem cells, tumors, and epigenetics.
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TruePrep DNA Library Prep Kit V2 for Illumina® is specifically designed for next-generation sequencing (NGS) onIllumina® platforms. This kit enables super fast and easy preparation of ready-to-use DNA library for sequencing by adopting a new-type transposase, which convert the complex steps of DNA fragmentation, end repair, dA-tailing, and adapter ligation into a one-step enzymatic reaction, significantly reducing the demanded amount of the initial DNA and shortening the time of library preparation. Three kinds of specifications of this kit are provided for different amount of input DNA: 50 ng(#TD501), 5 ng (#TD502), and 1 ng (#TD503).
Termékismertető megtekintése (angol)
TruePrep DNA Library Prep Kit V2 for Illumina® is specifically designed for next-generation sequencing (NGS) onIllumina® platforms. This kit enables super fast and easy preparation of ready-to-use DNA library for sequencing by adopting a new-type transposase, which convert the complex steps of DNA fragmentation, end repair, dA-tailing, and adapter ligation into a one-step enzymatic reaction, significantly reducing the demanded amount of the initial DNA and shortening the time of library preparation. Three kinds of specifications of this kit are provided for different amount of input DNA: 50 ng(#TD501), 5 ng (#TD502), and 1 ng (#TD503).
Termékismertető megtekintése (angol)
TruePrep DNA Library Prep Kit V2 for Illumina® is specifically designed for next-generation sequencing (NGS) onIllumina® platforms. This kit enables super fast and easy preparation of ready-to-use DNA library for sequencing by adopting a new-type transposase, which convert the complex steps of DNA fragmentation, end repair, dA-tailing, and adapter ligation into a one-step enzymatic reaction, significantly reducing the demanded amount of the initial DNA and shortening the time of library preparation. Three kinds of specifications of this kit are provided for different amount of input DNA: 50 ng(#TD501), 5 ng (#TD502), and 1 ng (#TD503).
Hyperactive pG-MNase for CUT&RUN fuses Protein G and Micrococcal nuclease (MNase) to form a novel fusion enzyme with their dual activities, and it is specifically for the CUT&RUN technology in protein-genome interaction research. CUT&RUN technology greatly simplifies the operation process of traditional ChIP-seq, only taking 1 – 1.5 days to construct the next-generation sequencing library from cells. Moreover, it has low cell input, high signal-to-noise ratio, good reproducibility, and is widely applied to research including early embryonic development, stem cells, tumors, and epigenetics.
Hyperactive pA-MNase for CUT&RUN fuses Protein A and Micrococcal nuclease (MNase) to form a novel fusion enzyme with their dual activities, and it is specifically for the CUT&RUN technology in protein-genome interaction research. CUT&RUN technology greatly simplifies the operation process of traditional ChIP-seq, only taking 1 – 1.5 days to construct the next- generation sequencing library from cells. Moreover, it has low cell input, high signal-to-noise ratio, good reproducibility, and is widely applied to research including early embryonic development, stem cells, tumors, and epigenetics.
