Leírás
Termékismertető megtekintése (angol)
HiScript II U+ One Step qRT-PCR Probe Kit is designed for quantitative PCR with RNA (such as RNA virus) as templates. Both cDNA synthesis and PCR are performed in a single tube using gene-specific primers (GSP), without extra opening pipes and pipetting, thus it increases the quantification throughput and minimizes the chance of contamination. The kit introduces dUTP/UDG anti-contamination system. Heat-labile UDG can degrade contamination with U rapidly at room temperature and will be inactivated at 55℃ during reverse transcription, without affecting efficiency and sensitivity of RT-PCR. Integrating the advantages of HiScript II Reverse Transcriptase, Champagne Taq DNA Polymerase and optimized buffer system, One Step qRT-PCR Probe Kit enables highly sensitive detection as low as 0.1 pg of total RNA or RNA with less than 10 copies. The kit is provided as convenient Master Mix. 2×One-Step U+ Mix contains optimized buffer, dNTP/dUTP Mix, and detection system for fluorescent probes (such as TaqMan®). One-Step U+ Enzyme Mix contains proportion-optimized HiScript II Reverse Transcriptase, RNase inhibitor, Heat-labile UDG and Champagne Taq DNA Polymerase.
