Leírás
Termékismertető megtekintése (angol)
BCA Protein Quantification Kit is one of the most sensitive methods for protein assays. In an alkaline condition, Cu2+ can be reduced to Cu+ by protein and interacts with the unique BCA Reagent A (containing BCA) forming a purple complex, known as the biuret reaction. The water-soluble complex has a strong absorbance at 562 nm, which has well linear correlation with protein concentration in a wide range. Therefore, a standard curve can be drawn by measuring the absorbance of BSA standard with Microplate reader at 562 nm and the concentration of protein samples can be calculated. The test results will not be affected by commonly used detergents such as SDS, Triton X-100 and Tween-20, but affected by chelating agents (EDTA, EGTA) , deoxidizer (DTT, mercaptoethanol) and lipids. In the assay, if the background of the sample diluent buffer or lysis buffer is high, Barford Protein Assay Kit can be tried.
