Amplicon Archives - SEQRS Kft.

VAHTS AmpSeq Library Prep Kit V3 (NA210)

Tibor.szekeres — Fri, 02 Dec 2022 16:03:31 +0000

VAHTS AmpSeq Library Prep Kit V3 is based on ultra-multiplex PCR, introducing several core technologies such as end primer digestion, to construct amplicon libraries. The entire library preparation workflow of this kit takes place in a single tube, the sequencing library can be obtained after purification. It takes as little as 5 h from amplification to the generated library, including less than 1.5 h of manual operation. The initial template for this kit is 1 – 100 ng. It is compatible with genomic DNA, FFPE samples, cfDNA, etc. This kit is intended for both the Illumina and Ion Torrent high-throughput sequencing platforms. And corresponding adapters (Vazyme #NA111/ NA121) are also provided. It is compatible with panels including Ion AmpliSeq Series Panel and AmpliSeq for Illumina Series Panel, as well as the corresponding individually customized panels. Compared to the previous generation (Vazyme #NA201), the library preparation and sequencing quality have been greatly improved. The optimized multiplex amplification module has good amplification performance for different input amounts and customized panels. The upgraded digestion module can effectively solve the problem of PCR products contamination and improve data utilization. The newest ligation module increases the effective library ratio. Comprehensive product upgrade ensures the high coverage and high uniformity of the amplicon library. With this kit, the sequencing results are more stable and reliable, helping researchers and testing personnel to complete high quality library preparation quickly and easily. All the reagents provided in the kit have undergone rigorous quality control and functional testing, to ensure the optimal stability and repeatability of library preparation.

Vazyme oldal megtekintése (angol)

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VAHTS AmpSeq Multi-PCR Module V3 (NA215)

Tibor.szekeres — Fri, 02 Dec 2022 16:03:34 +0000

Termékismertető megtekintése (angol)

VAHTS AmpSeq Multi-PCR Module V3 is an amplification reagent based on ultra-multiplex PCR technique to construct amplicon libraries. The initial template for this reagent is 1 – 100 ng. It is compatible with genomic DNA, FFPE samples, cfDNA, etc. This optimized reagent contains enzymes, dNTPs and reaction buffer required during multiplex PCR process, reducing the pipetting operations, making the result more stable. Compared to previous generation (Vazyme #NA205), this reagent has better amplification performance for different input amounts and customized panels. The upgrade ensures the high coverage and high uniformity of the amplicon library. This reagent is compatible with up to 7000 multiplex customized panels. This reagent is suitable for the library preparation for amplicon sequencing, helping researchers and testing personnel to complete ultra-multiplex PCR quickly and easily with high sequencing quality. The reagent has undergone rigorous quality control and functional testing, to ensure the optimal stability and repeatability of library preparation.

Vazyme oldal megtekintése (angol)

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VAHTS AmpSeq Library Prep Kit V2 (NA201)

Tibor.szekeres — Fri, 02 Dec 2022 16:03:31 +0000

Termékismertető megtekintése (angol)

VAHTS AmpSeq Library Prep Kit V2 is based on ultra-multiplex PCR, introducing several core technologies such as end primer digestion, to construct amplicon libraries. The entire library construction workflow of this kit takes place in a single tube, without intermediate purification steps. It takes as little as 5 h from amplification to the generated library, including less than 1.5 h of manual operation. The initial template for this kit is 1 – 100 ng. It is compatible with genomic DNA, FFPE samples, cfDNA, etc. This kit is intended for both the Illumina and Ion Torrent high-throughput sequencing platforms. And corresponding adapters (Vazyme #NA111/NA121) are also provided. It is compatible with panels including Ion AmpliSeq Series Panel and AmpliSeq for Illumina Series Panel, as well as the corresponding individually customized panels. Compared to the previous generation (Vazyme #NA101), this kit greatly improves the compatibility of samples such as FFPE and cfDNA, while ensuring the high coverage and high uniformity of the amplicon library. The optimized multiplex amplification mix reduces pipetting operations. The results of library construction are more stable and reliable, helping researchers and testing personnel to complete high quality amplicon library construction quickly and easily. All the reagents provided in the kit have undergone rigorous quality control and functional testing, to ensure the optimal stability and repeatability of library construction.

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VAHTS AmpSeq Adapters 1-24 for Illumina (1-12) (NA111-01)

Tibor.szekeres — Thu, 03 Feb 2022 09:59:24 +0000

Termékismertető megtekintése (angol)

VAHTS AmpSeq Adapters for Illumina is a special kit built by AmpSeq technology for Illumina high-throughput sequencing platform library, and can be used together with the AmpSeq library Preparation kit. Multi-sample targeted sequencing DNA libraries suitable for Illumina platform can be constructed. Kit NA111-01 contains Adapter 1 to Adapter 12, a total of 12 different index Adapters. Kit NA111-02 contains Adapter 13 to Adapter 24, a total of 12 different index Adapters; Kit NA111-03 contains Adapter 25 to Adapter 48, a total of 24 different index adapters; Kit NA111-04 contains Adapter 49 to Adapter 72, a total of 24 different index Adapters. Kit NA111-05 contains Adapter 73 to Adapter 96 for a total of 24 different index Adapters.

Vazyme oldal megtekintése (angol)

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