Murine RNase Inhibitor is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form. It can extensively inhibit various RNases (RNase A, B, C). Murine RNase Inhibitor has undergone RT-PCR and RT-qPCR tests and is compatible with HiScript II Reverse Transcriptase, HiScript Reverse Transcriptase, M-MLV (RNaseH-) Reverse Transcriptase and various DNA Polymerases. Compared with human-derived RNase inhibitor, mouse-derived RNase inhibitor does not contain two cysteines that are very sensitive to oxidation in human protein, so it has higher antioxidant activity and is more suitable for experiments sensitive to high DTT (Such as qPCR).

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HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) is an upgraded version of HiScript II 1st Strand cDNA Synthesis Kit (+gDNA wiper). It includes a new generation of reverse transcriptase HiScript III Reverse Transcriptase and optimized Buffer further improves the efficiency of one-strand synthesis. The 5 × gDNA wiper Mix in this kit can quickly remove genomic DNA contamination at 42°C for 2 min, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need of spanning exon junction to design primers. The kit contains single-component reverse transcription primers Oligo (dT)20VN and Random hexamers. Users can flexibly choose reverse transcription primers for subsequent experiments according to their needs. This kit can synthesize full-length cDNA (up to 20 kb) for downstream experiments such as cloning, and can also synthesize highly uniform cDNA for qPCR .

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HiScript II Reverse Transcriptase is a new generation reverse transcriptase optimized from the M-MLV (RNase H-) Reverse Transcriptase. Compared with the last generation reverse transcriptase, the thermo-stability of this product is significantly improved. The half-life of HiScript II Reverse Transcriptase at 50℃ is >240 min. At 55℃, it can also be stable for a long time, which significantly benifits the transcription of RNA templates with complex secondary structure. In addition, it has a improved template affinity and cDNA synthesis efficiency. It has a good resistance to most RT-PCR inhibitors and is suitable for long-fragment cDNA amplification (as long as 20 kb).

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HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology on the basis of M-MLV (RNase H-) Reverse Transcriptase. Compared with the previous generation of HiScript® Reverse Transcriptase, HiScript® II has further greatly improved thermal stability and is very suitable for reverse transcription of RNA templates with complex secondary structures. In addition, HiScript® II adds multiple point mutations, which further enhances template affinity and progress, and has higher tolerance to common reverse transcription inhibitors, which greatly improves the ability to synthesize full-length cDNA. HiScript® II 1st Strand cDNA Synthesis Kit is a first-strand cDNA synthesis kit based on HiScript® II Reverse Transcriptase. It contains all the components needed to synthesize high-quality first-strand cDNA. The product is suitable for subsequent PCR, qPCR and other experiments. 2 × RT Mix contains optimized buffer system and dNTP; HiScript® II Enzyme Mix contains HiScript® II Reverse Transcriptase and RNase inhibitor. The Oligo (dT)23VN included in the kit has a stronger anchoring ability for Poly A+ mRNA than Oligo (dT)18, making reverse transcription more efficient. Users can choose Oligo (dT)23VN, Random hexamers or gene-specific primers as reverse transcription primers according to their needs. The subsequent flexible and optional operation steps can not only synthesize full-length cDNA (up to 20 kb) for cloning, but also efficiently synthesize cDNA with uniform reverse transcription efficiency for each position of qPCR.

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HiScript III All-in-one RT SuperMix Perfect for qPCR is an upgraded version of HiScript III RT SuperMix for qPCR(+gDNA wiper). Genomic DNA elimination and reverse transcription can be simultaneously completed, which is convenient. It can greatly reduce contamination and RNA degradation. This kit contains HiScript III Reverse Transcriptase, heat-labile DNase and an optimized buffer system. Among them, heat-labile DNase takes effect quickly. It has high efficiency, and can be easily inactivated. The obtained cDNA can be stored stably for a long time. It is compatible with dye-based and probe-based qPCR, enabling high-performance gene expression analysis.

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HiScript II Reverse Transcriptase, optimized from M-MLV (RNase H-) Reverse Transcriptase, is a new generation reverse transcriptase with highly improved thermo stability and cDNA synthesis efficiency. The HiScript II Q RT SuperMix for qPCR (+gDNA wiper) is specially designed for 2-step qRT-PCR. The residual genomic DNA in RNA template can be removed rapidly and completely with the 4 × gDNA Wiper Mix. The 5 × HiScript qRT SuperMix II contains all necessary components required for reverse transcription. With the addition of template RNA and ddH2O, the reaction can proceed quickly. Meanwhile, gDNA wiper is terminated to ensure the integrity of the cDNA. This product has been specially optimized for qPCR. With the optimized proportion of Random primers/Oligo (dT)23VN primer mix, cDNA synthesis can start from each region of RNA transcript and have the same reverse transcription efficiency, which ensures the authenticity and reproducibility of qPCR results to the greatest extent. The cDNA products are compatible with SYBR Green and probe qPCR, and can be used with the corresponding reagent according to the experimental purpose to perform high-performance gene expression analysis.

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The Vazyme HiScript II Reverse Transcriptase, optimized from M-MLV (RNase H-) Reverse Transcriptase, is a new generation reverse transcriptase with highly improved heat stability and cDNA synthesis efficiency. The HiScript II Q Select RT SuperMix for qPCR is designed for 2-step RT-qPCR. The 5× HiScript II Select qRT SuperMix contains Buffer, dNTPs, HiScript II Reverse Transcriptase, and RNase inhibitor. The Oligo (dT)23VN has a better affinity to Ploy A+ RNA than Oligo (dT)18. In addition, random hexamers and gene-specific primers (GSP) are also optional.. The HiScript II Q Select RT SuperMix for qPCR has been specially optimized for qPCR. The cDNA products are compatible for SYBR- or probe-basded qPCR, such as AceQ qPCR SYBR Green Master Mix (Vazyme, #Q111), ChamQ SYBR qPCR Master Mix (Vazyme, #Q311), ChamQ Color SYBR qPCR Master Mix (Vazyme, #Q411), and AceQ qPCR Probe Master Mix (Vazyme, #Q112).

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This product is based on the cost-effective HiScript ® II Reverse Transcriptase and uses uniquely designed Oligo(dT) 23 VN primers to improve the specificity and sensitivity of the reaction and obtain better quality cDNA. Different types of reverse transcription primers can be used flexibly for different experimental designs to meet diverse experimental needs. Oligo (dT) 23 VN primer/Random Hexamers or gene-specific primers (GSP) can be selected according to the needs of subsequent reverse transcription experiments. At the same time, the reaction system is greatly simplified. 5 × qRT SuperMix contains all the reagents required for reverse transcription reaction except the primers and will not freeze at -20°C, making it easy to use. The 4 × gDNA wiper Mix in the kit can completely remove residual genomic DNA contamination, ensuring that the subsequent quantitative results are more reliable. The product obtained by reverse transcription can be used in SYBR ® Green qPCR analysis method and probe analysis method for high-performance gene expression analysis.

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HiScript II One Step RT-PCR Kit is specially designed for end-point PCR detection using RNA as template (such as RNA virus). Using gene-specific primers (GSP), reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which greatly improves detection throughput and reduces the risk of contamination. Integrate HiScript II Reverse Transcriptase and the superior performance of Champagne TaqTM plus DNA Polymerase designed for long fragment amplification, and cooperate with the optimized buffer system HiScript II One Step RT-PCR Kit to amplify fragments up to 10 kb or more. The kit is provided in a convenient Master Mix format. 2 × One Step Mix contains optimized buffer system and dNTP; One Step Enzyme Mix contains optimized ratio of HiScript II Reverse Transcriptase, RNase inhibitor and Champagne TaqTM plus DNA Polymerase.

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