AceQ Universal Probe Master Mix V2 adopts rigorous chemical modification Acetaq, with a carefully optimized buffer system, which can greatly improve the sensitivity of the probe method. At the same time, AceQ Universal Probe Master Mix V2 also uses a special ROX Passive Reference Dye, which is suitable for all qPCR instruments without the need to adjust the ROX concentration and is easy to use.

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HiScript II One Step qRT-PCR Probe Kit (Clean) is specially designed for qPCR detection using RNA (e.g., RNA virus) as templates. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube. No additional opening/pipetting operations are required, greatly increasing assay throughput and reducing the risk of contamination. Combining the superior performance of HiScript II Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase, equipping with an optimized buffer, the detection sensitivity of HiScript II One Step qRT-PCR Probe Kit (Clean) can reach 0.1 pg of total RNA or <10 copies of RNA templates. This product is provided as master mix. The 2 × One Step Mix contains an optimized buffer and dNTP/dUTP mix, and is suitable for high-specificity detection systems based on fluorescence labelled probes (e.g., TaqMan).

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The ExFect2000 Transfection Reagent is a novel and efficient transfection reagent based on cationic liposomes, which is suitable for DNA transfection and co-transfection systems for most eukaryotic cells (both adherent and suspended). The ExFect2000 has a unique structure and an optimized formulation. The presence of serum and antibiotics will not affect the efficiency of transfection, thereby reducing the effect of serum-deprival on cells. The ExFect2000 is also with low cytotoxicity. After transfection, there’s no need to remove nucleic acidExFect2000 complex or replace medium within 24 hr-48 hr. The high transfection efficiency of ExFect2000 transfection reagent has been validated in a board range of eukaryotic cell lines.

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ClonExpress Ultra One Step Cloning Kit is a new generation of recombinant cloning kits, compatible with 1 – 5 fragments homologous recombination. The unique ligase-independent system of this kit significantly reduces the self-ligation background of the vector. Restriction endonuclease sites do not need to be considered. Highly optimized 2 × ClonExpress Mix (It contains enhanced recombinase Exnase) can significantly improve the recombination efficiency and the tolerance to impurities. pCE-Zero vector is compatible with most PCR products, enabling the specific PCR products to be used directly for recombination without any treatments, which significantly simplify the procedure.

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The T7 High Yield Transcription Kit is an optimized system for high yield in vitro transcription of RNA from DNA templates containing T7 RNA Polymerase promoter. The kit contains T7 RNA Polymerase which can synthesize RNA quickly and easily from the downstream of the T7 promoter, and obtains a large amount of RNA. Modified nucleotide can be added to the system to generate biotin or dye-labeled RNA.This kit can yield 150 – 200 μg of RNA with a template input of 0.5 μg. The RNA yield can be widely used in many downstream applications such as RNA structure and function studies, RNase protection, probe hybridization, RNAi, microinjection and in vitro translation.

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T7 RNA polymerase can perform in vitro transcription of RNA from DNA templates containing T7 promoter using four NTPs as substrates. T7 RNAi Transcription Kit is an optimized version of T7 High Yield RNA Transcription Kit that is designed for transcription of double-stranded RNA. It can be used to transcribe 21 bp siRNA and long-fragment dsRNA. The purified transcripts can be used for RNAi experiments mediated by cationic liposome, calcium phosphate coprecipitation, electroporation, DEAE-Dextran and microinjection. In general, one reaction produces 20 – 80 μg RNA.

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RNA-easy Isolation Reagent is widely applicable to extraction of Total RNA and Small RNA from various animal tissues, plant materials, cultured cells, bacteria and other samples. Compared with the traditional Trizol extraction method, this product is simple to use, eliminating the need to use chloroform for phase separation, and the whole process can be carried out at room temperature. In addition, the product effectively inhibits RNase activity, while simultaneously precipitating impurities such as protein, DNA and polysaccharides to the bottom of the tube, so as to enable single-phase extraction and powerfully guarantee the integrity and purity of the RNA. The whole extraction process can be completed within 50 min with this product. The extracted RNA can be directly used in various molecular biology experiments such as cDNA cloning, qRT-PCR, mRNA purification, in vitro translation, Northern blotting hybridization, high-throughput sequencing and so on.

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Murine RNase Inhibitor is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form. It can extensively inhibit various RNases (RNase A, B, C). Murine RNase Inhibitor has undergone RT-PCR and RT-qPCR tests and is compatible with HiScript II Reverse Transcriptase, HiScript Reverse Transcriptase, M-MLV (RNaseH-) Reverse Transcriptase and various DNA Polymerases. Compared with human-derived RNase inhibitor, mouse-derived RNase inhibitor does not contain two cysteines that are very sensitive to oxidation in human protein, so it has higher antioxidant activity and is more suitable for experiments sensitive to high DTT (Such as qPCR).

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HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) is an upgraded version of HiScript II 1st Strand cDNA Synthesis Kit (+gDNA wiper). It includes a new generation of reverse transcriptase HiScript III Reverse Transcriptase and optimized Buffer further improves the efficiency of one-strand synthesis. The 5 × gDNA wiper Mix in this kit can quickly remove genomic DNA contamination at 42°C for 2 min, ensuring more reliable follow-up results, and simplifying qPCR primer design without the need of spanning exon junction to design primers. The kit contains single-component reverse transcription primers Oligo (dT)20VN and Random hexamers. Users can flexibly choose reverse transcription primers for subsequent experiments according to their needs. This kit can synthesize full-length cDNA (up to 20 kb) for downstream experiments such as cloning, and can also synthesize highly uniform cDNA for qPCR .

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