The ExFect2000 Transfection Reagent is a novel and efficient transfection reagent based on cationic liposomes, which is suitable for DNA transfection and co-transfection systems for most eukaryotic cells (both adherent and suspended). The ExFect2000 has a unique structure and an optimized formulation. The presence of serum and antibiotics will not affect the efficiency of transfection, thereby reducing the effect of serum-deprival on cells. The ExFect2000 is also with low cytotoxicity. After transfection, there’s no need to remove nucleic acidExFect2000 complex or replace medium within 24 hr-48 hr. The high transfection efficiency of ExFect2000 transfection reagent has been validated in a board range of eukaryotic cell lines.

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MycoBlue Mycoplasma Detector is designed for rapid detection of mycoplasma contamination in cell culture. It’s easy to use: after adding 1 μl of the cell culture supernatant to the reaction system and incubating at 60℃ for 1 h, the results can be determined by visual observation. Detection can be easily completed in cell culture lab without performing PCR, qPCR, or electrophoresis. Compared with conventional PCR methods, MycoBlue Mycoplasma Detector is more resistant to the inhibitor in the culture supernatant, avoiding weak positive and false negative. There’s no need to perform electrophoresis, avoiding false positive from aerosol contamination. The result is highly consistent with the most sensitive and accurate qPCR method.It has been validated that MycoBlue Mycoplasma Detector could detect up to 28 kinds of mycoplasma, including 8 commonly found strains in cell culture. This kit is suitable for mycoplasma detection in various types of suspension and adherent cells, including CHO, Vero, hybridoma, Sf9, HEK293, etc. This kit has a wide range of cell culture medium compatibility. It’s suitable for routine mycoplasma detection in biopharmaceutical companies, vaccine/monoclonal antibody manufacturers, cell therapy/embryo laboratories, and other scientific research laboratories.

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Myco-OffTM Mycoplasma Cleaner is the latest generation of mycoplasma removal reagent, mainly used to remove mycoplasma in cells, serum and culture media. This product is different from commonly used antibiotics. Its removal principle is to destroy the membrane structure of mycoplasma, causing the mycoplasma to rupture and die; it can effectively remove antibiotic-resistant mycoplasma without causing drug resistance; for common Gram-negative and Positive bacteria also have a certain inhibitory effect. After using this product for 3-7 days, the intracellular and extracellular mycoplasma can be completely removed, and there is no secondary pollution of mycoplasma within 4 months. Myco-OffTM Mycoplasma Cleaner can remove most types of mycoplasma without being toxic to the cell itself; it is suitable for commonly used cell lines such as mouse embryonic stem cells or iPS cells, human embryonic stem cells or iPS cells, HEK293, HeLa, HepG2, HCT116 , COS-7, Vero, Huh-7, MDCK, PANC-1, SW620 and U2OS etc.

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Apoptosis is a basic biological phenomenon of cells. It plays an important role in evolution, homeostasis and system development of organism. Some morphological, physiological or biochemical changes will happen during the apoptosis of cells, such as cell shrinkage, loss of contact between neighboring cells, loss of mitochondrial membrane potential, abnormal membrane permeability, chromatin condensation, nuclear fragmentation, DNA degradation; formation of membrane protrusions; phosphatidylserine’s turning out of membrane while the structure of cell membrane is complete. Finally, the cell breaks apart into multiple vesicles called apoptotic bodies, which will undergo phagocytosis. The above described morphological changes occur at different stages of the apoptosis.
A landmark of apoptosis is degradation of chromosome DNA. This specific and regular degradation produces DNA fragments in different lengths of an integer multiple of 180 bp to 200 bp. This is exactly the length of DNA strand that wraps histone. It suggests that the chromosomal DNA is cleaved at the junction between nucleosomes, producing oligonuclear fragments of different lengths. Experiments have shown that the regulated degradation of DNA is a result of an endogenous endonuclease, which cleaves chromosomal DNA at the junction of nucleosomes. Agarose gel electrophoresis shows a specific ladder pattern in apoptotic cells but a diffuse continuous pattern in dead cells.
Principle of the detection
This kit is based on TUNEL (TdT mediated dUTP Nick End Labeling) method. TdT (Terminal Deoxynucleotidyl Transferase) was used to catalyze the incorporation of FITC-12-dUTP at the 3′-OH terminus of the broken DNA in apoptotic cells. FITC-12-dUTP-labeled DNA can be directly observed by fluorescence microscopy or quantified by flow cytometry. This kit is optimized for the labeling reaction. It uses the best proportion of fluorescence marker and unlabeled dNTP for incorporation of the 3′-OH terminal nucleotides so that the ends of the same fragmented DNA fragment can form longer „marker tail”. The “marker tail” reduces the steric hindrance of labeling groups adjacently incorporated into dNTPs. It increases the number of fluorophores on each fragment and reduces the possibility of adjacent fluorophores’ aggregation and quenching. Thereby, the “marker tail” improves detection sensitivity and reduces nonspecific reactions.

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TUNEL BrightGreen Apoptosis Detection Kit is an upgraded version of TUNEL FITC Apoptosis Detection Kit (A111). The BrightGreen Labeling Mix in the kit contains FITC-12-dUTP and a Bright Factor. This unique small molecule compound can be non-covalently combined with FITC to enhance its stability and amplify its signal, thereby making the label brighter and having stronger anti-quenching ability. BrightGreen can keep strong fluorescence intensity under continuous irradiation of strong laser for 6 min.

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This kit uses the TUNEL (TdT mediated dUTP Nick End Labeling) method to catalyze the incorporation of terminal deoxyribonucleotide transferase (TdT) at the 3′-hydroxyl (3′-OH) end of the broken DNA of apoptotic cells Tetramethylrhodamine-deoxyuridine triphosphate (TMR red-dUTP). The kit optimizes the labeling reaction, using the best ratio of TMR red-dUTP and unlabeled dNTP to carry out the 3′-OH terminal nucleotide Incorporation, so that the end of the same broken DNA fragment can form a longer labeled tail.” This „labeled tail” reduces the steric hindrance of the labeling group on the adjacent incorporated dNTPs and increases the fluorescence on each broken fragment The number of groups reduces the aggregation and quenching that may be caused by adjacent fluorescent groups, thereby improving detection sensitivity and reducing non-specific reactions. The BrightRed Labeling Mix in the kit contains TMR red-dUTP and the patented Bright factor. This unique The small molecule compound of TMR red can be non-covalently combined with TMR red to enhance its stability and amplify its signal, thereby making the marker brighter and having stronger anti-quenching ability.

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Apoptosis is a basic biological phenomenon of cells. It plays an important role in evolution, homeostasis and system development of organism. Some morphological, physiological or biochemical changes will happen during the apoptosis of cells, such as cell shrinkage, loss of contact between neighboring cells, loss of mitochondrial membrane potential, abnormal membrane permeability, chromatin condensation, nuclear fragmentation, DNA degradation; formation of membrane protrusions; phosphatidylserine’s turning out of membrane while the structure of cell membrane is complete. Finally, the cell breaks apart into multiple vesicles called apoptotic bodies, which will undergo phagocytosis. The above described morphological changes occur at different stages of the apoptosis.
A landmark of apoptosis is degradation of chromosome DNA. This specific and regular degradation produces DNA fragments in different lengths of an integer multiple of 180 bp to 200 bp. This is exactly the length of DNA strand that wraps histone. It suggests that the chromosomal DNA is cleaved at the junction between nucleosomes, producing oligonuclear fragments of different lengths. Experiments have shown that the regulated degradation of DNA is a result of an endogenous endonuclease, which cleaves chromosomal DNA at the junction of nucleosomes. Agarose gel electrophoresis shows a specific ladder pattern in apoptotic cells but a diffuse continuous pattern in dead cells.
Principle of the detection
This kit is based on TUNEL (TdT mediated dUTP Nick End Labeling) method. TdT (Terminal Deoxynucleotidyl Transferase) was used to catalyze the incorporation of FITC-12-dUTP at the 3′-OH terminus of the broken DNA in apoptotic cells. FITC-12-dUTP-labeled DNA can be directly observed by fluorescence microscopy or quantified by flow cytometry. This kit is optimized for the labeling reaction. It uses the best proportion of fluorescence marker and unlabeled dNTP for incorporation of the 3′-OH terminal nucleotides so that the ends of the same fragmented DNA fragment can form longer „marker tail”. The “marker tail” reduces the steric hindrance of labeling groups adjacently incorporated into dNTPs. It increases the number of fluorophores on each fragment and reduces the possibility of adjacent fluorophores’ aggregation and quenching. Thereby, the “marker tail” improves detection sensitivity and reduces nonspecific reactions.

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Apoptosis is a basic biological phenomenon of cells. It plays an important role in evolution, homeostasis and system development of organism. Some morphological, physiological or biochemical changes will happen during the apoptosis of cells, such as cell shrinkage, loss of contact between neighboring cells, loss of mitochondrial membrane potential, abnormal membrane permeability, chromatin condensation, nuclear fragmentation, DNA degradation; formation of membrane protrusions; phosphatidylserine’s turning out of membrane while the structure of cell membrane is complete. Finally, the cell breaks apart into multiple vesicles called apoptotic bodies, which will undergo phagocytosis. The above described morphological changes occur at different stages of the apoptosis.
A landmark of apoptosis is degradation of chromosome DNA. This specific and regular degradation produces DNA fragments in different lengths of an integer multiple of 180 bp to 200 bp. This is exactly the length of DNA strand that wraps histone. It suggests that the chromosomal DNA is cleaved at the junction between nucleosomes, producing oligonuclear fragments of different lengths. Experiments have shown that the regulated degradation of DNA is a result of an endogenous endonuclease, which cleaves chromosomal DNA at the junction of nucleosomes. Agarose gel electrophoresis shows a specific ladder pattern in apoptotic cells but a diffuse continuous pattern in dead cells.
Principle of the detection
This kit is based on TUNEL (TdT mediated dUTP Nick End Labeling) method. TdT (Terminal Deoxynucleotidyl Transferase) was used to catalyze the incorporation of FITC-12-dUTP at the 3′-OH terminus of the broken DNA in apoptotic cells. FITC-12-dUTP-labeled DNA can be directly observed by fluorescence microscopy or quantified by flow cytometry. This kit is optimized for the labeling reaction. It uses the best proportion of fluorescence marker and unlabeled dNTP for incorporation of the 3′-OH terminal nucleotides so that the ends of the same fragmented DNA fragment can form longer „marker tail”. The “marker tail” reduces the steric hindrance of labeling groups adjacently incorporated into dNTPs. It increases the number of fluorophores on each fragment and reduces the possibility of adjacent fluorophores’ aggregation and quenching. Thereby, the “marker tail” improves detection sensitivity and reduces nonspecific reactions.

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The Dual Luciferase Reporter Assay Kit is used to detect gene regulation by transfecting cells with a reporter plasmid and measuring the fluorescence intensity of Luciferin substrate to reflect the level of Luciferase expression. To achieve dual luciferase reporter gene detection, Firefly luciferase is detected with Luciferin as a substrate, and Renilla Iuciferase is detected with coelenterazine as a substrate, while inhibiting the activity of Firefly luciferase. Dual Luciferase Reporter Assay Kit can detect the expression of Luciferase regulated by gene elements sensitively and efficiently. Usually, the transcriptional regulatory element is cloned upstream of Firefly luciferase, or the 3′-UTR regulatory region is cloned downstream of firefly luciferase. The transfected cells are induced by corresponding stimulator and lysed to determine the luciferase activity. The stimulatory-inducing effect of the regulatory elements is evaluated by luciferase activity. Renilla Iuciferase acts as an internal reference for correcting transfection efficiency to eliminate differences in cell number and transfection efficiency between wells. Firefly luciferase catalyzes the emission of Luciferin at 560 nm, and Renilla luciferase catalyzes the emission of coelenterazine at 465 nm.

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