Szállítási osztály: Normál

VAHTS Universal Plus Fragmentation Module (N209)
Termékismertető megtekintése (angol)
VAHTS Universal Plus Fragmentation, End Preparation & dA-Tailing Module for Illumina is specially designed for Illumina platforms. This kit combines DNA fragmentation, end repair and dA tailing into one step. It is suitable for library preparation from 100 pg – 1 μg of input DNA. With this kit, the input DNA amount is significantly decreased. No mechanical fragmentation of the genome simplifies the process of library construction and shortens the operation time. It is also compatible with automatic library preparation equipment. All the components are subjected to stringent quality control and functional test, ensuring the consistency and reproducibility of library preparation.
december 2, 2022
VAHTS DNA Damage Repair Kit (N208)
Termékismertető megtekintése (angol)
Metabolic processes in the body and environmental factors, including oxidation, hydrolysis, deamination, UV irradiation, radiation, and special chemical substances can cause DNA damage. The damage repair mechanism inherent in the body can repair most of the DNA damage, but the DNA damage caused by extraction during the research process will remain on the DNA. If it is not repaired, it will affect the subsequent experiment. VAHTS DNA Damage Repair Kit can be used to repair most DNA damage. The kit contains a variety of enzymes for DNA damage repairing and specially optimized buffer, suitable for a variety of DNA damages, such as apurine/apyrimidine sites, thymine dimers, de-aminocytosine, 8-oxoguanine, oxidized bases, nicks and gaps, deamination cytosine, 3′ terminal half group blocking, etc. This kit can be used to repair formalin fixed paraffin embedding (FFPE) DNA damage and DNA damage caused by various environments or processing methods. But it cannot be used to repair all kinds of DNA damages, such as double-strand breaks and DNA-protein crosslink. This kit can effectively improve the quality of DNA. It has a wide range of compatibility, and the products can be directly used in PCR and second-generation sequencing library construction, third-generation sequencing library construction, etc.
Vazyme oldal megtekintése (angol)
december 2, 2022
VAHTS Universal Adapter Ligation Module for Illumina (N204)
VAHTS Universal Adapter Ligation Module for Illumina V2 (VAHTS) was the igation module for oriented optimization of libraries for Illumina high-throughput sequencing platform. Using this kit, the dT protruding connector sequence can be efficiently ligated to the end of a repair product containing a phosphate group at the 5 ‘end and a dA protruding 3’ end. Compared with the conventional method, VAHTS Universal Adapter Ligation Module for Illumina V2 was used to obtain the single-tube reaction. The segmented DNA can be ligated directly after treatment with VAHTS Universal End preparation Module for Illumina V2 (N203-01/02) without additional product purification steps. Therefore, the requirement of initial DNA template is significantly reduced, and the experimental time is shortened. All reagents provided in the kit have undergone strict quality control and functional verification, which ensures the stability and repeatability of library preparation to a large extent.
Vazyme oldal megtekintése (angol)
december 2, 2022
VAHTS Universal End preparation Module for Illumina (N203)
Termékismertető megtekintése (angol)
VAHTSTM Universal Adapter Ligation Module for Illumina is a connection module optimized for Illumina high-throughput sequencing platform library preparation. Use this kit Linkers containing dT protrusions can be efficiently connected to the end of repair products containing phosphate groups at the 5’end and dA protrusions at the 3’end. Compared with the conventional method, the VAHTSTM Universal Adapter Ligation Module for Illumina uses a one-tube reaction. After the fragmented DNA is processed by the VAHTSTM Universal End Preparation Module for Illumina (Vazyme #N203), the adapter ligation reaction can be carried out directly without unnecessary products. Purification step. Therefore, the minimum requirement of the starting DNA template is significantly reduced, and the experiment time is shortened. All reagents provided in the kit have undergone strict quality control and functional verification, ensuring the stability and repeatability of library preparation to the greatest extent.
Vazyme oldal megtekintése (angol)
december 2, 2022
Phi29 MAX DNA Ploymerase (N106)
Termékismertető megtekintése (angol)
Phi29 MAX DNA Polymerase is a DNA polymerase cloned from Bacillus subtilis phage phi29. Phi29 MAX DNAPolymerase has strong strand displacement activity, which can realize the melting and replication of complex DNA structures, and perform isothermal DNA polymerization reactions in vitro that do not rely on thermal cycling. Phi29 MAX DNA Polymerase has strong chain affinity. A single polymerization reaction can achieve continuous polymerization extension up to 100 kb. In addition, Phi29 MAX DNA Polymerase also has a strong 3′-5′ exo-checking activity, which guarantees The fidelity is 1000 times that of Taq enzyme, which is higher than the fidelity of most current high-fidelity enzymes, which guarantees the high fidelity of DNA synthesis, and is very suitable for in vitro preparation of plasmids and complete gene assembly.
Vazyme oldal megtekintése (angol)
december 2, 2022
miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) (MR101)
Termékismertető megtekintése (angol)
miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) is a special kit suitable for stem-loop miRNA cDNA one-strand synthesis. It contains genomic DNA removal steps, which can quickly remove genomic DNA contamination at 42℃ for 2 min. The follow-up results are more reliable. The HiScrip II Reverse Transcriptase on which the kit is based has extremely high thermal stability, coupled with an optimized buffer system, which is conducive to the generation of miRNA-specific reverse transcription products. For subsequent quantification of cDNA products, it is recommended to use our company’s miRNA Universal SYBR qPCR Master Mix (Vazyme # MQ101) to obtain the best experimental results. At the same time, the design software for miRNA reverse transcription stem-loop primers and quantitative primers was launched, which is easy to operate and escort miRNA experiments.
Vazyme oldal megtekintése (angol)
december 2, 2022
miRNA Universal SYBR® qPCR Master Mix (MQ101)
Termékismertető megtekintése (angol)
This product is a special premix for miRNA quantitative reaction using SYBR Green I chimeric fluorescence method. Because miRNA sequences are short and miRNA sequences of the same family are often highly similar, the specificity requirements for quantification are extremely high. This product is based on the chemical hot-start AceTaq DNA Polymerase and is equipped with optimized Buffer, which can greatly reduce non-specific amplification. At the same time, the special ROX Reference Dye makes the master mix suitable for all qPCR instruments without adjusting the concentration of ROX on different instrument, just add primers and templates when preparing the reaction system to perform amplification.
Vazyme oldal megtekintése (angol)
december 2, 2022
100 bp DNA Ladder (MD104)
Termékismertető megtekintése (angol)
This product is composed of double-stranded DNA fragments of specific molecular weight, mixed with a sample buffer containing blue dye, and is suitable for agarose gel electrophoresis as a DNA molecular weight standard. All fragments in DNA Marker/Ladder are obtained from plasmids after digestion and purification. During electrophoresis, the Marker/Ladder bands are clearer and denser; the mass ratio between bands is more accurate and true. The DNA concentration of the 750 bp fragment in the DL2000 Plus DNA Marker, the 1,000 bp fragment in the DL5000 DNA Marker, the 500 bp fragment in the 100 bp DNA Ladder, and the 5 kb fragment in the 1 kb DNA Ladder is 100 ng/5 μl, and the display is bright band; the DNA concentration of all other bands is 50 ng/5 μl.
Vazyme oldal megtekintése (angol)
december 2, 2022
DL15000 DNA Marker (MD103)
Termékismertető megtekintése (angol)
This product is composed of double-stranded DNA fragments of specific molecular weight, mixed with a sample buffer containing blue dye, and is suitable for agarose gel electrophoresis as a DNA molecular weight standard. All fragments in DNA Marker/Ladder are obtained from plasmids after digestion and purification. During electrophoresis, the Marker/Ladder bands are clearer and denser; the mass ratio between bands is more accurate and true. The DNA concentration of the 750 bp fragment in the DL2000 Plus DNA Marker, the 1,000 bp fragment in the DL5000 DNA Marker, the 500 bp fragment in the 100 bp DNA Ladder, and the 5 kb fragment in the 1 kb DNA Ladder is 100 ng/5 μl, and the display is bright band; the DNA concentration of all other bands is 50 ng/5 μl.
Vazyme oldal megtekintése (angol)
december 2, 2022
DL5000 DNA Marker (MD102)
Termékismertető megtekintése (angol)
This product is composed of double-stranded DNA fragments of specific molecular weight, mixed with a sample buffer containing blue dye, and is suitable for agarose gel electrophoresis as a DNA molecular weight standard. All fragments in DNA Marker/Ladder are obtained from plasmids after digestion and purification. During electrophoresis, the Marker/Ladder bands are clearer and denser; the mass ratio between bands is more accurate and true. The DNA concentration of the 750 bp fragment in the DL2000 Plus DNA Marker, the 1,000 bp fragment in the DL5000 DNA Marker, the 500 bp fragment in the 100 bp DNA Ladder, and the 5 kb fragment in the 1 kb DNA Ladder is 100 ng/5 μl, and the display is bright band; the DNA concentration of all other bands is 50 ng/5 μl.
Vazyme oldal megtekintése (angol)
december 2, 2022
DL2000 Plus DNA Marker (MD101)
Termékismertető megtekintése (angol)
This product is composed of double-stranded DNA fragments of specific molecular weight, mixed with a sample buffer containing blue dye, and is suitable for agarose gel electrophoresis as a DNA molecular weight standard. All fragments in DNA Marker/Ladder are obtained from plasmids after digestion and purification. During electrophoresis, the Marker/Ladder bands are clearer and denser; the mass ratio between bands is more accurate and true. The DNA concentration of the 750 bp fragment in the DL2000 Plus DNA Marker, the 1,000 bp fragment in the DL5000 DNA Marker, the 500 bp fragment in the 100 bp DNA Ladder, and the 5 kb fragment in the 1 kb DNA Ladder is 100 ng/5 μl, and the display is bright band; the DNA concentration of all other bands is 50 ng/5 μl.
Vazyme oldal megtekintése (angol)
december 2, 2022
Ultra GelRed Nucleic Acid Stain (10000X) (GR501)
Termékismertető megtekintése (angol)
Ultra GelRed is a new fluorescent nucleic acid gel staining reagent that can replace ethidium bromide (EB). It has high sensitivity, low toxicity, and strong thermal stability. When using agarose gel electrophoresis to detect DNA, Ultra GelRed shows higher sensitivity to small amounts of DNA, especially small molecular weight DNA. DNA exhibits red fluorescence under ultraviolet light and can be used for staining of dsDNA, ssDNA and RNA in agarose and polyacrylamide gel electrophoresis.
Vazyme oldal megtekintése (angol)
december 2, 2022
Equalbit RNA BR Assay Kit (EQ212)
Termékismertető megtekintése (angol)
The Equalbit RNA BR (Broad-Range) Assay Kit is a simple, sensitive, and accurate RNA fluorescence quantitative detection kit. This kit contains fluorescence detection reagents, buffers, and dsDNA standards. This kit is highly selective for RNA and is not subject to dsDNA. It has an excellent linearity between 20 ng and 1000 ng for RNA samples, providing accurate quantification of total RNA, rRNA, mRNA samples from 1 ng /μl to 1000 ng /μl. This kit has excellent resistance for most conventional pollutants, including salt, free nucleotides, proteins, solvents, and detergents. The Equalbit RNA BR (Broad-Range) Assay Kit is easy to operate, and the assay can be performed at room temperature. Before use, please dilute the fluorescence detection reagent with buffer into a working solution, and then add the RNA sample for detection by a Qubit® fluorometer.
december 2, 2022
Equalbit RNA HS Assay Kit (EQ211)
Termékismertető megtekintése (angol)
Equalbit RNA HS (High Sensitivity) Assay Kit is a simple, sensitive, and accurate RNA fluorescence quantitative detection kit. This kit contains fluorescence detection reagents, buffers, and RNA standards. This kit is highly selective for RNA and is not affected by dsDNA. It has an excellent linearity between 5 ng and 100 ng for RNA samples, providing accurate quantification of total RNA, rRNA, mRNA samples from 250 pg/μl to 100 ng/μl. This kit has excellent resistance for most conventional contamination, including salt, free nucleotides, proteins, solvents, and detergents. It is easy to operate, and the assay can be performed at room temperature. Before use, please dilute the fluorescence detection reagent with buffer into a working solution, and then add the RNA sample for detection by a Qubit fluorometer.
Vazyme oldal megtekintése (angol)
december 2, 2022
Equalbit 1 × dsDNA HS Assay Kit (EQ121)
Termékismertető megtekintése (angol)
The Equalbit 1 × dsDNA HS (High Sensitivity) Assay Kit is a simple, sensitive, and precise fluorescence quantification kit for dou_x0002_ble-stranded DNA (dsDNA). The kit contains a pre-mixed working solution (with a fluorescent dye) and the dsDNA standard. It shows good linearity when the dsDNA content in the specimens is within the range of 0.2-100 ng and can precisely quantify dsDNA in 10 pg/μl-100 ng/μl specimens. The kit has good tolerance to common contaminants such as RNA, salts, free nucleotides, proteins, solvents, and detergents. The kit is easy to use: directly add an appropriate amount of the specimen to the working solution and assay using a Qubit fluorometer.
Vazyme oldal megtekintése (angol)
december 2, 2022
Equalbit dsDNA HS Assay Kit (EQ111)
Termékismertető megtekintése (angol)
The Equalbit dsDNA HS (High Sensitivity) Assay Kit is a simple, sensitive, and accurate double-stranded DNA (dsDNA) fluorescence quantitative detection kit. This kit contains fluorescence detection reagents, buffers, and dsDNA standards. This kit is highly selective for double-stranded DNA and is not subject to RNA. It has an excellent linearity between 0.2 ng and 100 ng for dsDNA samples, providing accurate quantification of samples from 10 pg / μl to 100 ng / μl. This kit has excellent resistance for most conventional pollutants, including salt, free nucleotides, proteins, solvents, and detergents. The Equalbit dsDNA HS (High Sensitivity) Assay Kit is easy to operate, and the assay can be performed at room temperature. Before use, please dilute the fluorescence detection reagent with buffer into a working solution, and then add the dsDNA sample for detection by a Qubit® fluorometer.
december 2, 2022
T7 Endonuclease I (EN303)
Termékismertető megtekintése (angol)
T7 Endonuclease I recognizes and cuts mismatched DNA, cruciform DNA, Holliday structures or junctions, and heteroduplex DNA. It can also recognize and cut double-stranded DNA with nicks with a lower speed. The enzyme cuts the first, second or third phosphodiester bond that is 5′ to the mismatch. Recombinant T7 Endonuclease I is purified from E.coli expression system. It has high purity and can be applicable for gene mutation, SNP and TALEN or CRISPR/Cas9-mediated mutation detection. It can recognize DNA mismatches, resolve four-way junction or branched DNA, detect heteroduplex or nicked DNA and randomly cleave linear DNA for shot-gun cloning.
Vazyme oldal megtekintése (angol)
december 2, 2022
Cas9 Nuclease (EN301)
Termékismertető megtekintése (angol)
Cas9 Nuclease is a double-strand DNA endonuclease that uses a single guide RNA (sgRNA) to specify the site of cleavage. sgRNA contains complementary region for specific DNA binding, and can lead the Cas9 Nuclease to the target DNA. Cas9 Nuclease contains two nuclease domains, which can cut each strand of DNA duplex to produce double strand break. The cutting site locates at 3 bp from NGGPAM of the complementary region of target DNA.
december 2, 2022
2× EpiArt HS Taq Master Mix (Dye Plus) (EM202)
Termékismertető megtekintése (angol)
2 × EpiArtTM HS Taq Master Mix is suitable for amplifying bisulfite-converted DNA, and contains EpiArtTM HS Taq DNA Polymerase, dNTP and an optimized buffer. Compared with common DNA, bisulfite conversion results in severe DNA damage and affects PCR performance. This product uses the enzyme-engineered Hot-Start EpiArtTM HS Taq DNA Polymerase and an optimized-buffer system to achieve superior amplification efficiency,sensitivity and specificity for bisulfite-converted DNA compared with common PCR reagents.The pre-formulated 2 × Master Mix can be used for PCR reactions by simply adding primers and templates to reduce pipetting and improve throughput and the reproducibility of results. EM202 contains a blue dye that can be directly electrophoresed after the reaction is completed, which is convenient to use.This product can be used with the EpiArtTM DNA Methylation Bisulfite Kit (Vazyme #EM101). The PCR products contain A at 3′-end, whichcan be directly cloned into the T vector and applied to ClonExpress Rapid Cloning Kit (Vazyme #C112/C113/C115) and 5 min TA/Blunt-Zero Cloning Kit(Vazyme #C601).
Vazyme oldal megtekintése (angol)
december 2, 2022
2× EpiArt HS Taq Master Mix (EM201)
2 × EpiArtTM HS Taq Master Mix is suitable for amplifying bisulfite-converted DNA, and contains EpiArtTM HS Taq DNA Polymerase, dNTP and an optimized buffer. Compared with common DNA, bisulfite conversion results in severe DNA damage and affects PCR performance. This product uses the enzyme-engineered Hot-Start EpiArtTM HS Taq DNA Polymerase and an optimized-buffer system to achieve superior amplification efficiency, sensitivity and specificity for bisulfite-converted DNA compared with common PCR reagents.This product can be used with the EpiArtTM DNA Methylation Bisulfite Kit (Vazyme #EM101). The PCR products contain A at 3′-end, whichcan be directly cloned into the T vector and applied to ClonExpress Rapid Cloning Kit (Vazyme #C112/C113/C115) and 5 min TA/Blunt-Zero Cloning Kit (Vazyme #C601).
Vazyme oldal megtekintése (angol)
december 2, 2022