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VAHTS DNA Adapters Set 1/2 for Illumina are specially designed for DNA library preparation for Illumina platforms. The set 1 (Cat.No. #N801) contains 12 kinds of indexed adapters (DNA Adapter 1 – 12). The set 2 (Cat.No. #N802) contains another 12 kinds of indexed adapters (DNA Adapter 13 – 27).
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Single Cell Full Length mRNA Amplification Kit can achieve enough amount of full-length cDNA through using 1 – 500 of cells or 10 pg – 10 ng of total RNA as templates. In this kit, Oligo (dT) VN Primer is used as reverse transcription primer and template-switching activity of Sc Reverse Transcriptase is utilized to add adapter sequences at the 3′ of cDNA. Subsequently, PCR amplification is performed through the adapter sequences to obtain full-length cDNA amplification products, which effectively avoids 3′ preference and rRNA contamination in the cDNA synthesis process. The obtained full-length cDNA amplification products can be used for genetic regulation information analysis such as differential gene expression, alternative splicing, and fusion genes. Single Cell Full Length mRNA Amplification Kit is compatible with sample volumes within 2.5 μl. Generally, 2 – 20 ng of cDNA amplification products can be obtained in one reaction.
Discover-sc WTA Kit V2 (N711) can use 1-1000 cells or 10 pg-10 ng total RNA as a template to obtain enough samples for sequence analysis through first-strand cDNA synthesis and amplification. It solves the technical problem that conventional mRNA-seq cannot be used for sequencing analysis of micro-samples such as single cells due to the low RNA content. N711 uses Oligo dTPrimer as the reverse transcription primer for cDNA synthesis, uses the Discover-sc Reverse Transcriptase Template-switching activity to add a linker sequence to the 3′ end of the cDNA, and then uses the linker sequence for subsequent PCR amplification to obtain full-length cDNA amplification product. It is highlighted for the uniform transcript coverage and more gene detections, and therefore more suitable for the detection of low-frequency genes and low-concentration templates.
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VAHTS HiFi Universal Amplification Mix for Illumina is an upgraded version for VAHTS HiFi Amplification Mix, which is applicable to high-throughput sequencing library PCR amplification. After modification on VAHTS HiFi DNA Polymerase, the template compatibility and sensitivity of this enzyme have been greatly improved; with an optimized buffer system, VAHTS HiFi Universal Amplification Mix for Illumina can amplify the library with high uniformity, increasing the sequencing quality. It contains special protective regents, which enable long-term storage and can maintain a stable activity after repeated freezing and thawing. VAHTS HiFi Universal Amplification Mix for Illumina has undergone rigorous quality control and functional testing to ensure optimal stability and repeatability.
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VAHTS HiFi Amplification Mix for MGI is a high fidelity PCR amplification master mix. The core component of this product is VAHTS HiFi DNA Polymerase. It is a new generation high yield and high fidelity DNA polymerase adapted from Pfu DNA Polymerase. It has greatly increased sensitivity, high amplification efficiency and extensive template compatibility. The amplification error rate of VAHTS HiFi DNA Polymerase is 52-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pfu DNA Polymerase, which ensures the fidelity. Matched with optimized buffer system, libraries with low preference, wide template input range, high yield, and high stability can be obtained. Except for the template, this kit contains all the components required for PCR amplification, thereby simplifying the operation process. This product contains special protective agent, the activity won’t be affected after long-term storage or repeated freezing and thawing. The products are blunt-ended and can be directly used for blunt-ended cloning.
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Discover-sc Single Cell WGA Kit is based on multiple displacement amplification (MDA) and is designed for unbiased whole genome amplification from single cell and other micro samples. The sizes of amplification products are between 2 kb to 100 kb, with a coverage greater than 95% and an average length higher than 15 kb. The products can be widely applied to whole genome sequencing, whole exome sequencing, large fragment copy number variation analysis, microsatellite analysis, qPCR analysis, or gene chip analysis.The Phi29 DNA polymerase used in this kit is cloned from phage and has a strong strand displacement activity. Melting and replication of the DNA chain with complex structure can be achieved. Meanwhile, Phi29 has a strong chain affinity and can achieve up to 100 kb of continuous polymerization, and the amplification product can be applied to almost all of the downstream genome analysis. Phi29 DNA polymerase also possesses a potent 3’→5′ exonuclease (proofreading) activity which ensures high fidelity in replication, which is 1,000-fold higher than that of Taq and higher than most of other high fidelity polymerases.
VAHTS 2 × Frag/Prime Buffer is a product optimized for the transcriptome library preparation of Illumina high-throughput sequencing platform. Under high temperature conditions, the RNA is randomly fragmented by the action of Mg2+.
RIb-off rRNA Depletion Kit V2 (Bacteria) is a kit for depleting rRNA from total RNA of Gram-positive and Gram-negative bacteria. This kit is suitable for the total RNA of Gram-positive bacteria and Gram-negative bacteria with the initial template amount of 0.01-1μg. The total RNA sample is processed by rRNA and probe hybridization, RNase H digestion, DNase I digestion and other steps, and finally the rRNA (including 16S and 23S rRNA) is depletd from the total RNA. Retaining mrnas and other non-coding Rnas; The kit is suitable for both intact and partially degraded RNA samples, and the resulting RNA can be used for mRNA and non-coding RNA (e.g., IncRNA) analysis. The optimized reaction system improves the depletional effect and shortens the process time.
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The Ribo-off rRNA depletion kit (Plant) is designed to deplete rRNA (including 5S, 18S and 25S rRNA) from total RNA of plant roots, seeds and leaves and to obtain mRNA and other non-coding RNA. This kit is suitable for removing rRNA from 1 μg – 5μg of plant total RNA. rRNA is depleted by rRNA-to- probe hybridization, RNase H digestion, and DNaseI digestion steps. The retained mRNA and other non-coding RNA can be used for LncRNA and other non-coding RNAs analysis.This kit contains digestion probes targeting of mitochondrial rRNA and chloroplast rRNA. It can efficiently remove major rRNAs from a variety of plant species and provide the most informative sequencing results. This kit is applicable for both intact and degraded RNA samples. The resulting rRNA-depleted RNA is suitable for RNA library preparation and other downstream experiments.
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Ribo-off Globin & rRNA Depletion Kit (Human/Mouse/Rat) is a kit for removing Globin mRNA and rRNA from total RNA in blood samples. This kit is compatible with 0.01 – 1 μg blood total RNA. The total RNA sample undergoes probe hybridization, RNase H digestion, DNase I digestion, etc. Finally, Globin mRNA and rRNA (including cytoplasmic rRNA and mitochondrial rRNA) are removed from total RNA, leaving other mRNA and non-coding RNA, which can be used for the analysis of non-coding RNA such as LncRNA. This kit is compatible with partially degraded RNA samples, and the obtained products are suitable for RNA library preparation and other experiments.
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The Ribo-off rRNA Depletion Kit (Bacteria) is designed to deplete rRNA (including 16S and 23S rRNA) from total RNA of Gram-positive and Gram-negative bacteria and to obtain mRNA and other non-coding RNA. This kit is suitable for both intact and degraded RNA samples (i.e. FFPE RNA) and can remove rRNA in total RNA of 1-5 µg. The obtained rRNA-depleted RNA can be used for analysis applications of mRNA and non-coding RNA (i.e. lncRNA) and other applications.
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The Ribo-off rRNA Depletion Kit (H/M/R) is designed to deplete rRNA (including cytoplasmic 28S, 18S, 5S rRNA, and mitochodrial 12S, 5.8S rRNA) from human, mouse, and rat total RNA preparations, while leaving mRNA and other non-coding RNA. This kit is suitable for both intact and degraded RNA samples (i.e. FFPE RNA). The obtained rRNA-depleted RNA can be used for analysis applications of mRNA and non-coding RNA (e.g. lncRNA).
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The Library Preparation VAHTS mRNA Capture Beads is 1 μm paramagnetic beads coupling of Oligo d (T) which is applicable to isolate intact poly (A)+ RNA from previously isolated total RNA. Magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly (A)+ transcripts in the eluent. The entire operation can be completed in less than 1 h.
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VAHTS Dual UMI UDI Adapters Set 1-Set 4 for Illumina is a DNA library preparation kit adapted to Illumina high-throughput sequencing platform. In the kit, VAHTS Dual UMI Adapters for Illumina are incomplete double-ended UMI-Y adapters that are compatible with library preparation kits with various T-A connections. After Adapters connection, 5-6 nt Unique Molecular Identifier(UMI) is added to each end of Insert DNA, which can be used to detect low-frequency mutations. In addition, the VAHTS UDI Primer for Illumina in the kit contains a Unique Dual Index(UDI) with specific matching. After library amplification, the double test of two completely independent indexes can be realized. Index hopping & Index misassignment is minimized to ensure that the reads that end up in the analysis flow are closest to the actual sample.This kit consists of Set 1-Set 4. Each Set contains 24 different VAHTS UDI Primer for Illumina and VAHTS Dual UMI Adapters for Illumina. All Adapters provided in the kit have undergone strict quality control and functional validation to maximize the stability and repeatability of the library preparation.
Termékismertető megtekintése (angol)
VAHTS Dual UMI UDI Adapters Set 1-Set 4 for Illumina is a DNA library preparation kit adapted to Illumina high-throughput sequencing platform. In the kit, VAHTS Dual UMI Adapters for Illumina are incomplete double-ended UMI-Y adapters that are compatible with library preparation kits with various T-A connections. After Adapters connection, 5-6 nt Unique Molecular Identifier(UMI) is added to each end of Insert DNA, which can be used to detect low-frequency mutations. In addition, the VAHTS UDI Primer for Illumina in the kit contains a Unique Dual Index(UDI) with specific matching. After library amplification, the double test of two completely independent indexes can be realized. Index hopping & Index misassignment is minimized to ensure that the reads that end up in the analysis flow are closest to the actual sample.This kit consists of Set 1-Set 4. Each Set contains 24 different VAHTS UDI Primer for Illumina and VAHTS Dual UMI Adapters for Illumina. All Adapters provided in the kit have undergone strict quality control and functional validation to maximize the stability and repeatability of the library preparation.
Termékismertető megtekintése (angol)
VAHTS Dual UMI UDI Adapters Set 1-Set 4 for Illumina is a DNA library preparation kit adapted to Illumina high-throughput sequencing platform. In the kit, VAHTS Dual UMI Adapters for Illumina are incomplete double-ended UMI-Y adapters that are compatible with library preparation kits with various T-A connections. After Adapters connection, 5-6 nt Unique Molecular Identifier(UMI) is added to each end of Insert DNA, which can be used to detect low-frequency mutations. In addition, the VAHTS UDI Primer for Illumina in the kit contains a Unique Dual Index(UDI) with specific matching. After library amplification, the double test of two completely independent indexes can be realized. Index hopping & Index misassignment is minimized to ensure that the reads that end up in the analysis flow are closest to the actual sample.This kit consists of Set 1-Set 4. Each Set contains 24 different VAHTS UDI Primer for Illumina and VAHTS Dual UMI Adapters for Illumina. All Adapters provided in the kit have undergone strict quality control and functional validation to maximize the stability and repeatability of the library preparation.
Termékismertető megtekintése (angol)
VAHTS Dual UMI UDI Adapters Set 1-Set 4 for Illumina is a DNA library preparation kit adapted to Illumina high-throughput sequencing platform. In the kit, VAHTS Dual UMI Adapters for Illumina are incomplete double-ended UMI-Y adapters that are compatible with library preparation kits with various T-A connections. After Adapters connection, 5-6 nt Unique Molecular Identifier(UMI) is added to each end of Insert DNA, which can be used to detect low-frequency mutations. In addition, the VAHTS UDI Primer for Illumina in the kit contains a Unique Dual Index(UDI) with specific matching. After library amplification, the double test of two completely independent indexes can be realized. Index hopping & Index misassignment is minimized to ensure that the reads that end up in the analysis flow are closest to the actual sample.This kit consists of Set 1-Set 4. Each Set contains 24 different VAHTS UDI Primer for Illumina and VAHTS Dual UMI Adapters for Illumina. All Adapters provided in the kit have undergone strict quality control and functional validation to maximize the stability and repeatability of the library preparation.
VAHTS Maxi Unique Dual Index DNA Adapters Set1-Set 4 for Illumina is a library preparation kit specially designed for Illumina high-throughput sequencing platform. It is suitable for multi-sample DNA library preparation on Illumina high-throughput sequencing platform. The kit contains Unique Dual Index(UDI) with specific matching, which can achieve two completely independent Index double testing and greatly reduce tag hopping and Index misassignment. Ensure that the reads entering the analysis flow are close to the real situation of the sample.This kit contains 384 different VAHTS Unique Dual Index Adapters. All adapters provided in the kit have gone through strict quality control and functional verification, which ensures the stability and repeatability of library preparation to a large extent.
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VAHTS Universal Plus Fragmentation, End Preparation & dA-Tailing Module for Illumina V2 is a DNA fragmentation, end repair and dAtailing module optimized for library preparation on Illumina high-throughput sequencing platforms. This kit has been optimized and upgraded from the original version. On the basis of maintaining the original high performance, the ratio of Artificial Invert Chimera Reads in the DNA library of FFPE samples has been significantly reduced, and the reliability of SNV detection has been improved. This kit can efficiently fragment DNA templates from 100 pg to 1 μg and perform end repair, phosphorylation of the 5′ end, and dA tailing of the 3′ end of the fragmented DNA. It significantly reduces the need for starting DNA templates, shortens the experiment time and is compatible with automated library building equipment. All reagents provided in the kit have undergone strict quality control and functional verification to ensure the stability and reproducibility of library preparation to the greatest extent.
