Termékismertető megtekintése (angol)
Champagne Taq DNA polymerase is a hot-start Taq polymerase obtained by mixing Champagne Taq antibody with Taq DNA polymerase in an optimal ratio. Due to the unique thermo stability of Champagne Taq antibody, the activity of Champagne Taq DNA polymerase is still blocked at temperature up to 55℃, which greatly prevents unspecific amplification. The DNA polymerase activity will be fully released at 95℃ for 30 sec, which ensures excellent sensitivity and specificity. The activation of Champagne Taq DNA polymerase is not affected by pH, ionic strength, etc. It is applicable for various hot-start PCR and qPCR and can be used to amplify gene with low copy numbers from complex templates (genome and cDNA). The 3′ end of the PCR products is A and the PCR products can be cloned into T-vector. It is suitable for ClonExpress and TOPO cloning kit (Vazyme #C112/C113/C115/C601)
Termékismertető megtekintése (angol)
Champagne Taq DNA polymerase is a hot-start Taq polymerase obtained by mixing Champagne Taq antibody with Taq DNA polymerase in an optimal ratio. Due to the unique thermo stability of Champagne Taq antibody, the activity of Champagne Taq DNA polymerase is still blocked at temperature up to 55℃, which greatly prevents unspecific amplification. The DNA polymerase activity will be fully released at 95℃ for 30 sec, which ensures excellent sensitivity and specificity. The activation of Champagne Taq DNA polymerase is not affected by pH, ionic strength, etc. It is applicable for various hot-start PCR and qPCR and can be used to amplify gene with low copy numbers from complex templates (genome and cDNA). The 3′ end of the PCR products is A and the PCR products can be cloned into T-vector. It is suitable for ClonExpress and TOPO cloning kit (Vazyme #C112/C113/C115/C601)
Termékismertető megtekintése (angol)
Champagne Taq DNA polymerase is a hot-start Taq polymerase obtained by mixing Champagne Taq antibody with Taq DNA polymerase in an optimal ratio. Due to the unique thermo stability of Champagne Taq antibody, the activity of Champagne Taq DNA polymerase is still blocked at temperature up to 55℃, which greatly prevents unspecific amplification. The DNA polymerase activity will be fully released at 95℃ for 30 sec, which ensures excellent sensitivity and specificity. The activation of Champagne Taq DNA polymerase is not affected by pH, ionic strength, etc. It is applicable for various hot-start PCR and qPCR and can be used to amplify gene with low copy numbers from complex templates (genome and cDNA). The 3′ end of the PCR products is A and the PCR products can be cloned into T-vector. It is suitable for ClonExpress and TOPO cloning kit (Vazyme #C112/C113/C115/C601)
Termékismertető megtekintése (angol)
Thermostable Taq monoclonal antibody with high specificity.
Termékismertető megtekintése (angol)
This kit contains a complete set of crude DNA extraction from animal tissues and QPCR amplification system with anti-pollution probe method, which is suitable for rapid genotyping of mice (Rapid Genotyping). This kit can be used to quickly release genomic DNA from mouse tails, ears, toes and other tissues. The products are directly amplified by QPCR, avoiding operations such as multiple open capping and pipetting. The genotype results are directly obtained by software analysis, avoiding manual checking The input introduces errors and can greatly reduce the time-consuming experiment. The dUTP/UDG anti-pollution system in the reagent can rapidly degrade U-containing pollutants at room temperature, significantly reducing aerosol pollution and improving the detection throughput and reproducibility of results.
Termékismertető megtekintése (angol)
One Step Mouse Genotyping Kit is specially designed for the rapid genotyping of mouse, which contains a complete set of reagents for DNA extraction and PCR amplification. This kit can be used for rapid extraction of genomic DNA from mouse tails, ears, toes, and other tissues. The extracted genomic DNA can be used directly as template for PCR amplification with no need of homogenization, crushing, overnight digestion, phenol-chloroform extraction, DNA precipitation or column purification operations, which greatly shortens the experimental time. For using, the tissue should be soaked in lysis buffer with Proteinase K, incubated at 55°C for 20 min, then heat at 95°C for 5 min to inactivate the Proteinase K. After centrifugation, the obtained lysate can be used as PCR template directly. After repeated tests, it is widely applicable to the amplification of target fragments within 2 kb, and is suitable for multiple PCR reactions within 4 pairs of primers. This Kit is equipped with 2 × Taq Plus Master Mix (Dye Plus), which includes high-performance Taq Plus DNA Polymerase, dNTP, and optimized buffer system. Only need to add primers and template in PCR reaction to perform amplification, which reduces operations, significantly reduces sample cross-contamination and improves detection throughput and results reproducibility. The unique protective agent allows Taq Plus Master Mix to maintain stable activity after repeated freezing and thawing. The system is premixed with electrophoresis buffer and loading dye, and electrophoresis can be carried out directly after the reaction, which is convenient. The PCR product has A-tailing at the 3′ end, which can be cloned into T vector and is suitable for ClonExpress and topological cloning kit (Vazyme #C112/C113/C115/C601)
Termékismertető megtekintése (angol)
TaqDNAPolymeraseisathermostableDNApolymerasethatexhibitsa5́→3́polymeraseactivityanda5́→3́exonucleaseactivity, withno3́→5́exonucleaseactivity.TaqDNAPolymeraseispurifedfromanEscherichiacoli(E.coli) strain overexpressing the gene of Thermus aquaticus DNA Polymerase. No endonuclease, exonuclease, or bacterial DNA were detected in this kit. The PCR products contain A at the 3′-end and can be directly cloned into T-Vectors. The products are also compatible with Vazyme ClonExpress II One Step Cloning Kit series (Vazyme, C#C112, #C113).
Termékismertető megtekintése (angol)
TaqDNAPolymeraseisathermostableDNApolymerasethatexhibitsa5́→3́polymeraseactivityanda5́→3́exonucleaseactivity, withno3́→5́exonucleaseactivity.TaqDNAPolymeraseispurifedfromanEscherichiacoli(E.coli) strain overexpressing the gene of Thermus aquaticus DNA Polymerase. No endonuclease, exonuclease, or bacterial DNA were detected in this kit. The PCR products contain A at the 3′-end and can be directly cloned into T-Vectors. The products are also compatible with Vazyme ClonExpress II One Step Cloning Kit series (Vazyme, C#C112, #C113).
Termékismertető megtekintése (angol)
TaqDNAPolymeraseisathermostableDNApolymerasethatexhibitsa5́→3́polymeraseactivityanda5́→3́exonucleaseactivity, withno3́→5́exonucleaseactivity.TaqDNAPolymeraseispurifedfromanEscherichiacoli(E.coli) strain overexpressing the gene of Thermus aquaticus DNA Polymerase. No endonuclease, exonuclease, or bacterial DNA were detected in this kit. The PCR products contain A at the 3′-end and can be directly cloned into T-Vectors. The products are also compatible with Vazyme ClonExpress II One Step Cloning Kit series (Vazyme, C#C112, #C113).
Termékismertető megtekintése (angol)
This product is expressed in Escherichia coli cloned with Thermus aquaticus DNA Polymerase gene and is purified through multiple steps. It does not contain endonuclease, exonuclease and bacterial DNA. Taq DNA Polymerase has 5’→3′ polymerase activity and 5’→3′ exonuclease activity, but no 3’→5′ exonuclease activity. The PCR product has A-tailing at the 3′ end, which can be cloned into the T vector, and is suitable for ClonExpress and topological cloning kit (Vazyme #C112/C113/C115/C601).
Termékismertető megtekintése (angol)
This product is expressed in Escherichia coli cloned with Thermus aquaticus DNA Polymerase gene and is purified through multiple steps. It does not contain endonuclease, exonuclease and bacterial DNA. Taq DNA Polymerase has 5’→3′ polymerase activity and 5’→3′ exonuclease activity, but no 3’→5′ exonuclease activity. The PCR product has A-tailing at the 3′ end, which can be cloned into the T vector, and is suitable for ClonExpress and topological cloning kit (Vazyme #C112/C113/C115/C601).
Termékismertető megtekintése (angol)
This product is expressed in Escherichia coli cloned with Thermus aquaticus DNA Polymerase gene and is purified through multiple steps. It does not contain endonuclease, exonuclease and bacterial DNA. Taq DNA Polymerase has 5’→3′ polymerase activity and 5’→3′ exonuclease activity, but no 3’→5′ exonuclease activity. The PCR product has A-tailing at the 3′ end, which can be cloned into the T vector, and is suitable for ClonExpress and topological cloning kit (Vazyme #C112/C113/C115/C601).
Termékismertető megtekintése (angol)
VAHTS Library Quantification Kit for Illumina is specially designed for accurate quantification of the concentration of a DNA library for Illumina high-performance sequencing platform using SYBR Green qPCR. The kit includes DNA standard of known concentration that is used to generate a standard curve. VAHTS SYBR qPCR Master Mix included in this kit is a new-type qPCR mix based on antibody modified hot-start DNA polymerase, which is of high specificity, high amplification efficiency, wide GC content adaptability, and high sensitivity. All the reagents provided in the kit have undergone rigorous quality control and functional testing, to ensure the optimal stability and repeatability of library preparation.
Termékismertető megtekintése (angol)
VAHTS Library Quantification Kit for Illumina is specially designed for accurate quantification of the concentration of a DNA library for Illumina high-performance sequencing platform using SYBR Green qPCR. The kit includes DNA standard of known concentration that is used to generate a standard curve. VAHTS SYBR qPCR Master Mix included in this kit is a new-type qPCR mix based on antibody modified hot-start DNA polymerase, which is of high specificity, high amplification efficiency, wide GC content adaptability, and high sensitivity. All the reagents provided in the kit have undergone rigorous quality control and functional testing, to ensure the optimal stability and repeatability of library preparation.
Termékismertető megtekintése (angol)
VAHTS Library Quantification Kit for Illumina is specially designed for accurate quantification of the concentration of a DNA library for Illumina high-performance sequencing platform using SYBR Green qPCR. The kit includes DNA standard of known concentration that is used to generate a standard curve. VAHTS SYBR qPCR Master Mix included in this kit is a new-type qPCR mix based on antibody modified hot-start DNA polymerase, which is of high specificity, high amplification efficiency, wide GC content adaptability, and high sensitivity. All the reagents provided in the kit have undergone rigorous quality control and functional testing, to ensure the optimal stability and repeatability of library preparation.
Termékismertető megtekintése (angol)
VAHTS Library Quantification Kit for Illumina is specially designed for accurate quantification of the concentration of a DNA library for Illumina high-performance sequencing platform using SYBR Green qPCR. The kit includes DNA standard of known concentration that is used to generate a standard curve. VAHTS SYBR qPCR Master Mix included in this kit is a new-type qPCR mix based on antibody modified hot-start DNA polymerase, which is of high specificity, high amplification efficiency, wide GC content adaptability, and high sensitivity. All the reagents provided in the kit have undergone rigorous quality control and functional testing, to ensure the optimal stability and repeatability of library preparation.
Termékismertető megtekintése (angol)
VAHTS Library Quantification Kit for Illumina is specially designed for accurate quantification of the concentration of a DNA library for Illumina high-performance sequencing platform using SYBR Green qPCR. The kit includes DNA standard of known concentration that is used to generate a standard curve. VAHTS SYBR qPCR Master Mix included in this kit is a new-type qPCR mix based on antibody modified hot-start DNA polymerase, which is of high specificity, high amplification efficiency, wide GC content adaptability, and high sensitivity. All the reagents provided in the kit have undergone rigorous quality control and functional testing, to ensure the optimal stability and repeatability of library preparation.
