Leírás
Termékismertető megtekintése (angol)
HiScript III RT SuperMix for qPCR (+gDNA wiper) is an upgraded version of HiScript II Q RT SuperMix for qPCR (+gDNA wiper), including HiScript III Reverse Transcriptase, a new generation of reverse transcriptase with optimized Buffer. This kit further improves the efficiency of cDNA synthesis, and is suitable for two-step qRT-PCR detection. The 4 × gDNA wiper Mix in the kit can remove residual genomic DNA from the RNA template, ensuring more reliable quantitative results. It simplifies qPCR primer design without the need of spanning exon junction to design primers; 5 × HiScript III qRT SuperMix contains all components required for the reverse transcription reaction and it can be started rapidly after the addition of template RNA and RNase-free ddH2O, and the gDNA wiper is terminated to ensure the integrity of the cDNA. It is compatible with dye-based and probe-based qPCR, enabling high-performance gene expression analysis.