5 min TOPO-Blunt Cloning Kit (C602)

Tibor.szekeres — Fri, 02 Dec 2022 16:03:07 +0000

This product is the third generation TOPO cloning kit, which contains Topoisomerase, vector and self-ligation inhibitor. The kit uses a new type of Topoisomerase, with the optimal buffer, the enzyme activity is higher, and the high-efficiency ligation can be completed in 5 minutes. The addition of a vector self-ligation inhibitor increases the positive rate of clones. This product is suitable for blunt-end cloning.

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5min TA/Blunt-Zero Cloning Kit (C601)

Tibor.szekeres — Fri, 02 Dec 2022 16:03:07 +0000

Termékismertető megtekintése (angol)

This product is the second-generation TOPO cloning kit, which contains the second-generation Topoisomerase, a vector containing the suicide gene ccdB, and a blunt-end factor. Using the second-generation Topoisomerase, with the most suitable buffer, it has higher activity and further improves the cloning efficiency. The efficient connection is completed in 5 minutes at room temperature. Using a vector containing the suicide gene ccdB, when the insert is successfully connected to the vector, the correct expression of ccdB is destroyed, and the host cell can grow normally, otherwise the host cell cannot grow normally, thus achieving a „Zero” background. The addition of blunt-end factor makes this product compatible with TA cloning and blunt-end cloning.

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The post 5min TA/Blunt-Zero Cloning Kit (C601) appeared first on SEQRS Kft..

Topo Cloning Archives - SEQRS Kft.

5 min TOPO-Blunt Cloning Kit (C602)

5min TA/Blunt-Zero Cloning Kit (C601)