HiScript III One Step qRT-PCR Probe Kit is specially designed for qPCR that directly use RNA (e.g. virus RNA) as template. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. It can be inactivated at 55℃ without affecting the efficiency and sensitivity of qRT-PCR. Combining the superior performance of HiScript III Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase, with an optimized buffering system, the detection sensitivity of HiScript III One Step qRT-PCR Probe Kit can reach 0.1 pg of total RNA or less than 10 copies of RNA templates and has higher thermal stability. HiScript III One Step qRT-PCR Probe Kit is provided in Master Mix. The 5 × One Step Mix contains an optimized buffer and dNTP/dUTP Mix, and is suitable for high-specificity detection systems based on fluorescence labelled probes (e.g. TaqMan).

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HiScript Il OneStep qRT-PCR SYBR Green Kit is designed for SYBR Green I-based qPCR that directly use RNA as template. Using gene specific primers (GSP), the reverse transcription and PCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. This kit integrates the superior performance of HiScript Il Reverse Transcriptase and Champagne Taq DNA Polymerase with optimized buffer, which enables the detection sensitivity of One Step qRT-PCR SYBR Green Kit to reach 1 pg total RNA. The enhancement factor added in the buffer system can effectively reduce the formation of primer dimer and improve thes pecificity of products. This kit provides a convenient Master Mix form for easy and quick operation.

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The Vazyme HiScript II One Step qRT-PCR Probe Kit is specially designed for qPCRs that directly use RNA as templates. The reverse transcription and PCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. The HiScript II Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase contained this kit enables high-sensitive total RNA detection (as little as 1 pg). The HiScript II One Step qRT-PCR Probe Kit is a master mix system. The 2× One Step Q Probe Mix contains an optimized buffer and dNTP/dUTP mix and is suitable for high-sensitive detection systems based on fluorescence labelled probes (i.e. TaqMan® ).

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HiScript II U+ One Step qRT-PCR Probe Kit is designed for quantitative PCR with RNA (such as RNA virus) as templates. Both cDNA synthesis and PCR are performed in a single tube using gene-specific primers (GSP), without extra opening pipes and pipetting, thus it increases the quantification throughput and minimizes the chance of contamination. The kit introduces dUTP/UDG anti-contamination system. Heat-labile UDG can degrade contamination with U rapidly at room temperature and will be inactivated at 55℃ during reverse transcription, without affecting efficiency and sensitivity of RT-PCR. Integrating the advantages of HiScript II Reverse Transcriptase, Champagne Taq DNA Polymerase and optimized buffer system, One Step qRT-PCR Probe Kit enables highly sensitive detection as low as 0.1 pg of total RNA or RNA with less than 10 copies. The kit is provided as convenient Master Mix. 2×One-Step U+ Mix contains optimized buffer, dNTP/dUTP Mix, and detection system for fluorescent probes (such as TaqMan®). One-Step U+ Enzyme Mix contains proportion-optimized HiScript II Reverse Transcriptase, RNase inhibitor, Heat-labile UDG and Champagne Taq DNA Polymerase.

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