This product is a special premix for miRNA quantitative reaction using SYBR Green I chimeric fluorescence method. Because miRNA sequences are short and miRNA sequences of the same family are often highly similar, the specificity requirements for quantification are extremely high. This product is based on the chemical hot-start AceTaq DNA Polymerase and is equipped with optimized Buffer, which can greatly reduce non-specific amplification. At the same time, the special ROX Reference Dye makes the master mix suitable for all qPCR instruments without adjusting the concentration of ROX on different instrument, just add primers and templates when preparing the reaction system to perform amplification.

Vazyme oldal megtekintése (angol)

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miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) is a special kit suitable for stem-loop miRNA cDNA one-strand synthesis. It contains genomic DNA removal steps, which can quickly remove genomic DNA contamination at 42℃ for 2 min. The follow-up results are more reliable. The HiScrip II Reverse Transcriptase on which the kit is based has extremely high thermal stability, coupled with an optimized buffer system, which is conducive to the generation of miRNA-specific reverse transcription products. For subsequent quantification of cDNA products, it is recommended to use our company’s miRNA Universal SYBR qPCR Master Mix (Vazyme # MQ101) to obtain the best experimental results. At the same time, the design software for miRNA reverse transcription stem-loop primers and quantitative primers was launched, which is easy to operate and escort miRNA experiments.

Vazyme oldal megtekintése (angol)

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