Vazyme LAmp DNA Polymerase is a mixed enzyme composed of Taq DNA Polymerase and protein with 3’→5′ exonuclease activity (proofreading activity), and its fidelity is 6 times that of Taq DNA Polymerase. With specially optimized buffer system, Vazyme LAmp DNA Polymerase is very suitable for the amplification of long fragments. It can amplify fragments up to 21 kb from the genome, and has high amplification efficiency for templates of different sources and different lengths. PCR products have A-tailing at the 3′ end, which can be cloned into the T vector, and are suitable for ClonExpress and TOPO cloning kits (Vazyme #C112/C113/C115/C601). The attached PCR Enhancer facilitates the amplification of fragments with high GC content.

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2× Vazyme LAmp Master Mix is a blend of Taq DNA Polymerase and a DNA proofreading polymerase with 3’ to 5’ exonuclease activity. Its fidelity was 6-fold higher than conventional Taq DNA Polymerase. Used with the optimized buffer system, 2× Vazyme LAmp Master Mix is applicable to long PCR products, up to 21 kb. This Master Mix is also able to amplify long fragments accurately from templates of different sources or different length. 2× Vazyme LAmp Master Mix contains Vazyme LAmp DNA Polymerase, dNTP, and optimized buffer. The reaction can be started by adding only primers and template, which simplifies the operation, improves through-put, and enhances result reproducibility. The protective agents included guarantees the stability of the activity of this Master Mix. The PCR product, containing dA at 3′-end, can be cloned into T-vector, and is suitable for One Step Express cloning kit (C112/C113).

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2× Vazyme LAmp Master Mix is a blend of Taq DNA Polymerase and a DNA proofreading polymerase with 3’ to 5’ exonuclease activity. Its fidelity was 6-fold higher than conventional Taq DNA Polymerase. Used with the optimized buffer system, 2× Vazyme LAmp Master Mix is applicable to long PCR products, up to 21 kb. This Master Mix is also able to amplify long fragments accurately from templates of different sources or different length. 2× Vazyme LAmp Master Mix contains Vazyme LAmp DNA Polymerase, dNTP, and optimized buffer. The reaction can be started by adding only primers and template, which simplifies the operation, improves through-put, and enhances result reproducibility. The protective agents included guarantees the stability of the activity of this Master Mix. The PCR product, containing dA at 3′-end, can be cloned into T-vector, and is suitable for One Step Express cloning kit (C112/C113).

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