AceTaq® DNA Polymerase is a chemically modified Taq DNA Polymerase whose activity is completely blocked at room temperature to prevent non-specific amplification and primer dimers during sample preparation and reaction temperature rise. The activity is released only after heating at 95°C. Compared with antibody-based hot-start Taq enzymes, AceTaq® DNA Polymerase has a more complete and stricter activity closure; compared with existing chemically modified hot-start Taq enzymes, the activation time of AceTaq® DNA Polymerase only takes 5 minutes. Compatible with existing PCR programs. AceTaq® DNA Polymerase combined with an optimized buffer system can minimize non-specific amplification and primer dimers, bring the highest sensitivity, and is very suitable for amplifying low-copy genes from complex templates (genome, cDNA). The PCR product has an A at the 3’ end, which can be cloned into the T vector and is suitable for ClonExpress® Rapid Cloning Kit (C112-C113).

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This product includes AceTaq® DNA Polymerse, dNTPs, and an optimized buffer system that allows for amplification by simply adding primers and templates, reducing pipetting and improving throughput and reproducibility. A protective agent added to the amplification system allows the 2 × AceTaq® Master Mix to maintain stable activity after repeated freeze-thaw cycles. This product is available in a version containing electrophoresis buffer and dye. It can be directly electrophoresed after the reaction. The 3′ end of the PCR product, A, can be directly cloned into the T vector and used in the ClonExpress® Cloning Kit (C112/C113/C114).

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AceTaq DNA Polymerase is a chemically modified Taq DNA Polymerase that is completely blocked at room temperature and is released only after heating at 95℃. Non-specific amplification and primer dimerization can be prevented during sample preparation and temperature rise. Compared with the antibody-based hot-start Taq, the polymerase activity of AceTaq DNA Polymerase is blocked more stringently and completely. It takes only 5 min to activate AceTaq DNA Polymerase. AceTaq DNA Polymerase is compatible with most existing PCR protocols. Combined with an optimized buffer system, AceTaq DNA Polymerase minimizes non-specific amplification and primer dimers, ensuring extremely high sensitivity and specificity, which make it ideal for amplifying low-copy genes from complex templates. The PCR products contain A at the 3′ end and can be directly cloned into T-Vector. The products are compatible with ClonExpress and Top Cloning kits (Vazyme #C112/C113/C115/C601).

The post AceTaq DNA Polymerase (P401) appeared first on SEQRS Kft..

This product includes AceTaq® DNA Polymerse, dNTPs, and an optimized buffer system that allows for amplification by simply adding primers and templates, reducing pipetting and improving throughput and reproducibility. A protective agent added to the amplification system allows the 2 × AceTaq® Master Mix to maintain stable activity after repeated freeze-thaw cycles. This product is available in a version containing electrophoresis buffer and dye. It can be directly electrophoresed after the reaction. The 3′ end of the PCR product, A, can be directly cloned into the T vector and used in the ClonExpress® Cloning Kit (C112/C113/C114).

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Champagne Taq DNA polymerase is a hot-start Taq polymerase obtained by mixing Champagne Taq antibody with Taq DNA polymerase in an optimal ratio. Due to the unique thermo stability of Champagne Taq antibody, the activity of Champagne Taq DNA polymerase is still blocked at temperature up to 55℃, which greatly prevents unspecific amplification. The DNA polymerase activity will be fully released at 95℃ for 30 sec, which ensures excellent sensitivity and specificity. The activation of Champagne Taq DNA polymerase is not affected by pH, ionic strength, etc. It is applicable for various hot-start PCR and qPCR and can be used to amplify gene with low copy numbers from complex templates (genome and cDNA). The 3′ end of the PCR products is A and the PCR products can be cloned into T-vector. It is suitable for ClonExpress and TOPO cloning kit (Vazyme #C112/C113/C115/C601)

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Thermostable Taq monoclonal antibody with high specificity.

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