The T7 High Yield Transcription Kit is an optimized system for high yield in vitro transcription of RNA from DNA templates containing T7 RNA Polymerase promoter. The kit contains T7 RNA Polymerase which can synthesize RNA quickly and easily from the downstream of the T7 promoter, and obtains a large amount of RNA. Modified nucleotide can be added to the system to generate biotin or dye-labeled RNA.This kit can yield 150 – 200 μg of RNA with a template input of 0.5 μg. The RNA yield can be widely used in many downstream applications such as RNA structure and function studies, RNase protection, probe hybridization, RNAi, microinjection and in vitro translation.

Vazyme oldal megtekintése (angol)

The post T7 High Yield RNA Transcription Kit (TR101) appeared first on SEQRS Kft..

T7 RNA polymerase can perform in vitro transcription of RNA from DNA templates containing T7 promoter using four NTPs as substrates. T7 RNAi Transcription Kit is an optimized version of T7 High Yield RNA Transcription Kit that is designed for transcription of double-stranded RNA. It can be used to transcribe 21 bp siRNA and long-fragment dsRNA. The purified transcripts can be used for RNAi experiments mediated by cationic liposome, calcium phosphate coprecipitation, electroporation, DEAE-Dextran and microinjection. In general, one reaction produces 20 – 80 μg RNA.

Vazyme oldal megtekintése (angol)

The post T7 RNAi Transcription Kit (TR102) appeared first on SEQRS Kft..

Cas9 Nuclease is a double-strand DNA endonuclease that uses a single guide RNA (sgRNA) to specify the site of cleavage. sgRNA contains complementary region for specific DNA binding, and can lead the Cas9 Nuclease to the target DNA. Cas9 Nuclease contains two nuclease domains, which can cut each strand of DNA duplex to produce double strand break. The cutting site locates at 3 bp from NGGPAM of the complementary region of target DNA.

The post Cas9 Nuclease (EN301) appeared first on SEQRS Kft..

T7 Endonuclease I recognizes and cuts mismatched DNA, cruciform DNA, Holliday structures or junctions, and heteroduplex DNA. It can also recognize and cut double-stranded DNA with nicks with a lower speed. The enzyme cuts the first, second or third phosphodiester bond that is 5′ to the mismatch. Recombinant T7 Endonuclease I is purified from E.coli expression system. It has high purity and can be applicable for gene mutation, SNP and TALEN or CRISPR/Cas9-mediated mutation detection. It can recognize DNA mismatches, resolve four-way junction or branched DNA, detect heteroduplex or nicked DNA and randomly cleave linear DNA for shot-gun cloning.

Vazyme oldal megtekintése (angol)

The post T7 Endonuclease I (EN303) appeared first on SEQRS Kft..