VAHTS AmpSeq Library Prep Kit V3 is based on ultra-multiplex PCR, introducing several core technologies such as end primer digestion, to construct amplicon libraries. The entire library preparation workflow of this kit takes place in a single tube, the sequencing library can be obtained after purification. It takes as little as 5 h from amplification to the generated library, including less than 1.5 h of manual operation. The initial template for this kit is 1 – 100 ng. It is compatible with genomic DNA, FFPE samples, cfDNA, etc. This kit is intended for both the Illumina and Ion Torrent high-throughput sequencing platforms. And corresponding adapters (Vazyme #NA111/ NA121) are also provided. It is compatible with panels including Ion AmpliSeq Series Panel and AmpliSeq for Illumina Series Panel, as well as the corresponding individually customized panels. Compared to the previous generation (Vazyme #NA201), the library preparation and sequencing quality have been greatly improved. The optimized multiplex amplification module has good amplification performance for different input amounts and customized panels. The upgraded digestion module can effectively solve the problem of PCR products contamination and improve data utilization. The newest ligation module increases the effective library ratio. Comprehensive product upgrade ensures the high coverage and high uniformity of the amplicon library. With this kit, the sequencing results are more stable and reliable, helping researchers and testing personnel to complete high quality library preparation quickly and easily. All the reagents provided in the kit have undergone rigorous quality control and functional testing, to ensure the optimal stability and repeatability of library preparation.

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TruePrep DNA Library Prep Kit V2 for Illumina® is specifically designed for next-generation sequencing (NGS) onIllumina® platforms. This kit enables super fast and easy preparation of ready-to-use DNA library for sequencing by adopting a new-type transposase, which convert the complex steps of DNA fragmentation, end repair, dA-tailing, and adapter ligation into a one-step enzymatic reaction, significantly reducing the demanded amount of the initial DNA and shortening the time of library preparation. Three kinds of specifications of this kit are provided for different amount of input DNA: 50 ng(#TD501), 5 ng (#TD502), and 1 ng (#TD503).

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TruePrep DNA Library Prep Kit V2 for Illumina® is specifically designed for next-generation sequencing (NGS) onIllumina® platforms. This kit enables super fast and easy preparation of ready-to-use DNA library for sequencing by adopting a new-type transposase, which convert the complex steps of DNA fragmentation, end repair, dA-tailing, and adapter ligation into a one-step enzymatic reaction, significantly reducing the demanded amount of the initial DNA and shortening the time of library preparation. Three kinds of specifications of this kit are provided for different amount of input DNA: 50 ng(#TD501), 5 ng (#TD502), and 1 ng (#TD503).

The post TruePrep DNA Library Prep Kit V2 for Illumina (TD502) appeared first on SEQRS Kft..

TruePrep DNA Library Prep Kit V2 for Illumina® is specifically designed for next-generation sequencing (NGS) onIllumina® platforms. This kit enables super fast and easy preparation of ready-to-use DNA library for sequencing by adopting a new-type transposase, which convert the complex steps of DNA fragmentation, end repair, dA-tailing, and adapter ligation into a one-step enzymatic reaction, significantly reducing the demanded amount of the initial DNA and shortening the time of library preparation. Three kinds of specifications of this kit are provided for different amount of input DNA: 50 ng(#TD501), 5 ng (#TD502), and 1 ng (#TD503).

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VAHTS Universal Plus DNA Library Prep Kit for Illumina® is specially designed for DNA library preparation for next generation sequencing (NGS) on Illumina® platforms. This kit combines DNA fragmentation, end repair and dA tailing into one step. The products can be directly used to adapter Ligation without purification, then for library enrichment and size selection. This kit is suitable for library preparation from 100 pg – 1 μg of input DNA. No mechanical fragmentation of the genome simplifies the process of library construction and shortens the operation time, suitable for the construction of PCR and PCR-Free libraries. This kit is perfectly compatible with DNA from different sources and different inputs, and the required fragment size library can be obtained by adjusting the fragmentation time according to the size of the target insert. All kit components are subjected to stringent functional quality control, ensuring the consistency and reproducibility of library preparation.

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VAHTS Universal Plus DNA Library Prep Kit for Illumina V2 is a DNA library preparation kit featuring enzyme-based fragmentation specifically developed for Illumina high-throughput sequencing platforms. This kit is an upgraded version of the original one. It significantly reduces the proportion of Artificial Invert Chimera Reads in the DNA library of FFPE samples and improves the reliability of the detection of SNV and other biomarkers while maintaining the original high performance. The kit combines DNA fragmentation, end repair, and dA-tailing into one step. The adapter ligation, library amplification, and size selection can be performed directly without additional purification. The 100 pg to 1 μg template DNA can be converted into the library suitable for Illumina high-throughput sequencing platforms. The kit is perfectly compatible with DNA from different sources and ofdifferent input amounts, and the user only needs to adjust the fragmentation time based on the target insert size to obtain a library with the desired fragment size.

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VAHTS Universal Pro DNA Library Prep Kit for Illumina is specially designed for library preparation for next generation sequencing (NGS) on Illumina® platforms, containing FFPE DNA repair modules, suitable for library preparation from 100 pg – 1 μg of input DNA. This kit contains a DNA damage repair module that can effectively repair DNA damage caused by formalin-fixed paraffin-embedded (FFPE), such as deamination of cytosine, nicks and gaps, oxidized bases, blocked 3´ ends, etc, compatible with common DNA samples without affecting the quality of normal DNA sample libraries. Through the overall improvement of the module of end-repair, ligation and library amplification, the library conversion rate and the amplification output are greatly improved. It is widely applicable to PCR or PCR-Free library construction of multiple samples, and is compatible with targeted capture process.

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VAHTS AmpSeq Multi-PCR Module V3 is an amplification reagent based on ultra-multiplex PCR technique to construct amplicon libraries. The initial template for this reagent is 1 – 100 ng. It is compatible with genomic DNA, FFPE samples, cfDNA, etc. This optimized reagent contains enzymes, dNTPs and reaction buffer required during multiplex PCR process, reducing the pipetting operations, making the result more stable. Compared to previous generation (Vazyme #NA205), this reagent has better amplification performance for different input amounts and customized panels. The upgrade ensures the high coverage and high uniformity of the amplicon library. This reagent is compatible with up to 7000 multiplex customized panels. This reagent is suitable for the library preparation for amplicon sequencing, helping researchers and testing personnel to complete ultra-multiplex PCR quickly and easily with high sequencing quality. The reagent has undergone rigorous quality control and functional testing, to ensure the optimal stability and repeatability of library preparation.

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