EpiArt DNA Methylation Library Kit for Illumina V3 is a specific methylation library preparation kit targeted at Illumina high-throughput sequencing platform.Based on efficient single-strand ligation technology, the kit converts 10 pg-250 ng of methylated DNA into a dedicated library for the Illumina platform and is compatible with DNA samples as short as 40 bp. The optimized database building process can be completed within 2 h, and the manual operation time is less than 30 min. It is suitable for automatic database building and compatible with capture process. All reagents in the kit underwent strict quality control and functional verification, which ensured the stability and repeatability of library preparation to the greatest extent.

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2 × EpiArtTM HS Taq Master Mix is suitable for amplifying bisulfite-converted DNA, and contains EpiArtTM HS Taq DNA Polymerase, dNTP and an optimized buffer. Compared with common DNA, bisulfite conversion results in severe DNA damage and affects PCR performance. This product uses the enzyme-engineered Hot-Start EpiArtTM HS Taq DNA Polymerase and an optimized-buffer system to achieve superior amplification efficiency,sensitivity and specificity for bisulfite-converted DNA compared with common PCR reagents.The pre-formulated 2 × Master Mix can be used for PCR reactions by simply adding primers and templates to reduce pipetting and improve throughput and the reproducibility of results. EM202 contains a blue dye that can be directly electrophoresed after the reaction is completed, which is convenient to use.This product can be used with the EpiArtTM DNA Methylation Bisulfite Kit (Vazyme #EM101). The PCR products contain A at 3′-end, whichcan be directly cloned into the T vector and applied to ClonExpress Rapid Cloning Kit (Vazyme #C112/C113/C115) and 5 min TA/Blunt-Zero Cloning Kit(Vazyme #C601).

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