Kits Archives - SEQRS Kft.

Discover-sc Single Cell WGA Kit (N603)

Tibor.szekeres — Fri, 02 Dec 2022 16:03:28 +0000

Discover-sc Single Cell WGA Kit is based on multiple displacement amplification (MDA) and is designed for unbiased whole genome amplification from single cell and other micro samples. The sizes of amplification products are between 2 kb to 100 kb, with a coverage greater than 95% and an average length higher than 15 kb. The products can be widely applied to whole genome sequencing, whole exome sequencing, large fragment copy number variation analysis, microsatellite analysis, qPCR analysis, or gene chip analysis.The Phi29 DNA polymerase used in this kit is cloned from phage and has a strong strand displacement activity. Melting and replication of the DNA chain with complex structure can be achieved. Meanwhile, Phi29 has a strong chain affinity and can achieve up to 100 kb of continuous polymerization, and the amplification product can be applied to almost all of the downstream genome analysis. Phi29 DNA polymerase also possesses a potent 3’→5′ exonuclease (proofreading) activity which ensures high fidelity in replication, which is 1,000-fold higher than that of Taq and higher than most of other high fidelity polymerases.

Vazyme oldal megtekintése (angol)

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Discover-sc WTA Kit V2 (N711)

Tibor.szekeres — Fri, 02 Dec 2022 16:03:28 +0000

Discover-sc WTA Kit V2 (N711) can use 1-1000 cells or 10 pg-10 ng total RNA as a template to obtain enough samples for sequence analysis through first-strand cDNA synthesis and amplification. It solves the technical problem that conventional mRNA-seq cannot be used for sequencing analysis of micro-samples such as single cells due to the low RNA content. N711 uses Oligo dTPrimer as the reverse transcription primer for cDNA synthesis, uses the Discover-sc Reverse Transcriptase Template-switching activity to add a linker sequence to the 3′ end of the cDNA, and then uses the linker sequence for subsequent PCR amplification to obtain full-length cDNA amplification product. It is highlighted for the uniform transcript coverage and more gene detections, and therefore more suitable for the detection of low-frequency genes and low-concentration templates.

Vazyme oldal megtekintése (angol)

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Discover-sc Single Cell Kit (N601)

Tibor.szekeres — Fri, 02 Dec 2022 16:03:27 +0000

Coming soon…

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