The Vazyme RNA Isolater Total RNA Extraction Reagent uses guanidinium isothiocyanate and phenol, with high lysis ability. It can lyse cells and tissues in short time, keeping integrity of RNA. The RNA isolater Total RNA Extraction Reagent is widely used for RNA extraction of cultured cells, animal tissues, microorganisms, and plant tissues with weak secondary metabolism (i.e. seedling and spire). Samples will be lysed thoroughly in RNA isolater and form supernatant, interlayer, and organic layer (bright red sublayer). RNA is distributed in the upper water phase. Remove the RNA-containing supernatant layer and treat with isopropanol to extract the total RNA.

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The Bacteria RNA Extraction Kit is a kit optimized for RNA extraction from prokaryotes, including Gram-negative and Gram-positive bacteria. The kit includes a bacterial RNA Plus Reagent containing an optimized buffer system, a chelating agent and a mild protein denaturant, and an RNA isolater Total RNA Extraction Reagent that efficiently inhibits endogenous RNase and promotes protein denaturation. The combination of the two can greatly improve the extraction yield of prokaryotic RNA while ensuring good product quality. When extracting bacterial RNA, a high-temperature pretreatment is added only before the routine operation, and no high-quality and high-yield bacterial total RNA can be obtained without mechanical disruption or enzymatic hydrolysis steps. This kit is also suitable for the extraction of total RNA from fungi such as yeast.

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Vazyme oldal megtekintése (angol)

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