Cas9 Nuclease is a double-strand DNA endonuclease that uses a single guide RNA (sgRNA) to specify the site of cleavage. sgRNA contains complementary region for specific DNA binding, and can lead the Cas9 Nuclease to the target DNA. Cas9 Nuclease contains two nuclease domains, which can cut each strand of DNA duplex to produce double strand break. The cutting site locates at 3 bp from NGGPAM of the complementary region of target DNA.

The post Cas9 Nuclease (EN301) appeared first on SEQRS Kft..

T7 Endonuclease I recognizes and cuts mismatched DNA, cruciform DNA, Holliday structures or junctions, and heteroduplex DNA. It can also recognize and cut double-stranded DNA with nicks with a lower speed. The enzyme cuts the first, second or third phosphodiester bond that is 5′ to the mismatch. Recombinant T7 Endonuclease I is purified from E.coli expression system. It has high purity and can be applicable for gene mutation, SNP and TALEN or CRISPR/Cas9-mediated mutation detection. It can recognize DNA mismatches, resolve four-way junction or branched DNA, detect heteroduplex or nicked DNA and randomly cleave linear DNA for shot-gun cloning.

Vazyme oldal megtekintése (angol)

The post T7 Endonuclease I (EN303) appeared first on SEQRS Kft..