ClonExpress Ultra One Step Cloning Kit is a new generation of recombinant cloning kits, compatible with 1 – 5 fragments homologous recombination. The unique ligase-independent system of this kit significantly reduces the self-ligation background of the vector. Restriction endonuclease sites do not need to be considered. Highly optimized 2 × ClonExpress Mix (It contains enhanced recombinase Exnase) can significantly improve the recombination efficiency and the tolerance to impurities. pCE-Zero vector is compatible with most PCR products, enabling the specific PCR products to be used directly for recombination without any treatments, which significantly simplify the procedure.

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Vazyme ClonExpress II One Step Cloning is a simple, fast, and high efficient cloning technology and enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly, the vector is linearized at the cloning site. A small sequence overlapped with each end of the cloning site is added onto the insert through PCR. The insert and the linearized vector, with overlapped sequences of 15 bp – 20 bp on both 5’- and 3’-end, respectively, are mixed in an appropriate ratio and incubated with recombinase Exnase II at 37℃ for 30 min.
The ClonExpress II is a novel cloning Kit, independent of DNA ligase, significantly reducing the vself-ligated colonies and bringing a true positive rate > 95%. The enhanced Exnase II and highly optimized buffer significantly improve the recombination efficiency and the tolerance to impurities.Both the linearized vector and the PCR products of insert can be directly used for recombination without purification, significantly simplifying the procedures.

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ClonExpress® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector. Use any method to linearize the vector, and perform forward/reverse PCR on the insert. The 5’end of the primer is introduced into the end sequence of the linearized vector, so that the 5’and 3’ends of the PCR product have the same sequence (15 bp-20 bp) as the two ends of the linearized vector. After the PCR product of the sequence and the linearized vector are mixed in a certain proportion, under the catalysis of the recombinase, the reaction can be carried out at 37°C for 30 min to complete the directional cloning.
As a new generation of recombination cloning kit, ClonExpress® MultiS is specifically optimized for multi-fragment recombination reactions, which can realize sequential splicing cloning of up to five inserts at a time. The unique non-ligase-dependent system of the kit greatly reduces the background of vector self-ligation, and does not need to consider the enzyme cleavage site carried by the insert itself; the highly optimized reaction buffer and the enhanced recombinase Exnase MultiS can significantly improve The recombination efficiency of the clone and the tolerance to impurities make it possible to directly use the linearized vector and insert for recombination cloning without purification, which greatly simplifies the experimental steps.

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