HiScript III One Step qRT-PCR Probe 5 × Master Mix is specially designed for single-plex or multiplex qPCR detection using RNA (e.g. virus RNA) as template. It has extremely high stability, which can be stored at 37℃ for 14 days and its performance remains unchanged, and remains liquid form at a storage temperature of – 20℃ to avoid the thawing process of reagents. The reaction can be started by directly adding the template, primers and target probes required for detection. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. It can be inactivated at 55℃ without affecting the efficiency and sensitivity of qRT-PCR. Combining the superior performance of HiScript III Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase, with an optimized buffering system, the detection sensitivity of HiScript III One Step qRT-PCR Probe 5 × Master Mix can reach 0.1 pg of total RNA or less than 10 copies of RNA templates and is suitable for high-specificity detection systems based on fluorescence labelled probes (e.g. TaqMan).

Vazyme oldal megtekintése (angol)

The post HiScript III One Step qRT-PCR Probe 5× Master Mix (Q611) appeared first on SEQRS Kft..

AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE) is a one-tube probe-based RT-qPCR master mix suitable for single or multiplex quantitative PCR detection using RNA as template (such as RNA virus). The enzyme in this master mix uses a hot-start technology based on enzyme modification, with a new generation of high-affinity Taq enzyme antibodies, to achieve efficient blocking and precise release.
Combined with one-step reverse transcriptase and optimized chemical buffer, it supports pre-mixing of primers and probes in advance and the mix can be stored for a long time at low temperature. The sample to be tested can be added directly when using, no additional tube opening/pipetting operation. The anti-contamination system in this product can function at room temperature to eliminate the influence of other contamination on qPCR and ensure the accuracy of the results. This product is also compatible with fast programs, which greatly shortens the detection time.

Vazyme oldal megtekintése (angol)

The post AccurSTART One Step RT-qPCR Super PreMix (ONE TUBE) (Q621) appeared first on SEQRS Kft..

Taq Pro HighGC Multiple Probe qPCR Mix is a master mix for probe qPCR to detect DNA templates (such as DNA viruses). The core component, Taq Pro HS DNA Polymerase, is a new generation hot-start DNA polymerase that has been modified based on antibody techniques and upgraded to improve template affinity. Equipped with the most suitable Buffer optimized for the qPCR system, amplification specificity and sensitivity for detection of low-copy genes are significantly improved. It can provide excellent amplification curve within a wide quantitative range, and accurately quantify and detect target genes, with good repeatability and high reliability. This product is suitable for the amplification of rich GC templates and has good impurity tolerance. The reagent incorporates an anti-contamination system, which works at room temperature to eliminate the effect of amplification product contamination on qPCR to ensure the accuracy of the results. The product is a 2 × master mix. Only primers, probes and templates need to be added additionally. It is convenient to use and compatible with fast program to reduce test time.

Vazyme oldal megtekintése (angol)

The post Taq Pro HighGC Multiple Probe qPCR Mix (QN211) appeared first on SEQRS Kft..

Taq Pro Multiple Probe qPCR Mix is a master mix for probe qPCR to detect DNA templates (such as DNA viruses). The core component, Taq Pro HS DNA Polymerase, is a new generation hot start DNA polymerase that has been modified based on antibody technique and upgraded to improve template affinity. Equipped with the most suitable buffer optimized for the qPCR system, amplification performance with multiple targets, amplification specificity and sensitivity for detection of low-copy genes and amplification curve shape are significantly improved. It can provide excellent amplification curve within a wide quantitative range, and accurately quantify and detect target genes, with good repeatability and high reliability. It has wide compatibility in terms of template type, template GC content, and primer Tm values. And it also has good tolerance to impurities and is suitable for use in a variety of testing scenarios. The product is a 2 × master mix. Only primers, probes and templates need to be added additionally. It is convenient to use and compatible with fast program to reduce test time.

Vazyme oldal megtekintése (angol)

The post Taq Pro Multiple Probe qPCR Mix (QN213) appeared first on SEQRS Kft..