VAHTS HiFi Universal Amplification Mix for MGI(SI) is an updated version of VAHTS HiFi Amplification Mix, which is widely applicable to PCR amplification of high-throughput sequencing (NGS) libraries. Through the directed evolution of VAHTS HiFi DNA Polymerase, the compatibility and amplification sensitivity of the template were improved. Combined with buffer chemical formulation optimization, VAHTS HiFi Universal Amplification Mix for MGI(SI) can achieve highly homogeneous library amplification, which significantly improves sequencing quality. This product contains special protective agent, long-term storage or repeated freezing and thawing can still maintain stable expansion performance. VAHTS HiFi Universal Amplification Mix for MGI(SI) ensures the stability and repeatability of library preparation to a large extent through strict quality control and functional verification.
Termékismertető megtekintése (angol)
VAHTS HiFi Amplification Mix for MGI is a high fidelity PCR amplification master mix. The core component of this product is VAHTS HiFi DNA Polymerase. It is a new generation high yield and high fidelity DNA polymerase adapted from Pfu DNA Polymerase. It has greatly increased sensitivity, high amplification efficiency and extensive template compatibility. The amplification error rate of VAHTS HiFi DNA Polymerase is 52-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pfu DNA Polymerase, which ensures the fidelity. Matched with optimized buffer system, libraries with low preference, wide template input range, high yield, and high stability can be obtained. Except for the template, this kit contains all the components required for PCR amplification, thereby simplifying the operation process. This product contains special protective agent, the activity won’t be affected after long-term storage or repeated freezing and thawing. The products are blunt-ended and can be directly used for blunt-ended cloning.
Termékismertető megtekintése (angol)
VAHTS Dual UMI UDB Adapters Set 1-Set 8 for MGI is a special kit for library preparation of BGI high-throughput sequencing platform. It applies to multi-sample DNA library preparation of BGI high-throughput sequencing platform. The kit includes VAHTS Dual UMI Adapters for MGI UDB and VAHTS Unique Dual Barcode Primer for MGI. In the kit, VAHTS Dual UMI Adapters for MGI UDB is an incomplete double-ended UMI adapter that is compatible with a variety of T-A connected library preparation kits. A 5-6 nt Unique Molecular Identifier(UMI) was added to each end of Insert DNA afterAdapters connection, which could be used to detect low-frequency mutations. The VAHTS Unique Dual Barcode Primer for MGI contains Unique Dual Barcode(UDB) with specific collocation. After library amplification, double test of two completely independent indexes can be realized. Index hopping & Index misassignment can be minimized to ensure that Reads entering the analysis process are close to the real situation of the sample.
Termékismertető megtekintése (angol)
VAHTS RNA Adapters Set 8 for MGI is a special kit for library preparation of BTU high-throughput sequencing platform, applicable to multi-sample DNA library preparation of BTU high-throughput sequencing platform. The Set 8 kit (NM208) contains 96 different Index adapters. All connectors provided in the kit have undergone strict quality control and functional verification, which ensures the stability and repeatability of library preparation to a large extent.
VAHTS® Circularization Kit For MGI is a modular kit optimized for the high-throughput sequencing platform of MGI. Using this kit, the PCR products after linker connection can be prepared into a single-stranded circular DNA library dedicated to MGI high-throughput sequencer. All reagents provided in the kit have undergone strict quality control and functional verification to ensure the stability and reproducibility of library construction to the greatest extent.
VAHTS PCR-Free DNA Adapters for MGI is a library preparation kit for BTU high-throughput sequencing platform, which is applicable to multi-sample PCR-Free DNA library preparation for BTU high-throughput sequencing platform. Kits Set 1-Set 4 contain 96 different Index connectors. All connectors provided in the kit have undergone strict quality control and functional verification, which ensures the stability and repeatability of library preparation to a large extent.
Termékismertető megtekintése (angol)
VAHTS DNA Adapters set8 for MGI is a specialized kit for MGI high-throughput sequencing platform. It is suitable for preparing multi-sample DNA libraries for MGI high-throughput sequencing platform. This kit contains 96 different types of index adapters. All the adapters provided in the kit have undergone rigorous quality control and functional testing to ensure the optimal stability and repeatability of library preparation.
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EpiArt DNA Methylation Library Kit for Illumina V3 is a specific methylation library preparation kit targeted at Illumina high-throughput sequencing platform.Based on efficient single-strand ligation technology, the kit converts 10 pg-250 ng of methylated DNA into a dedicated library for the Illumina platform and is compatible with DNA samples as short as 40 bp. The optimized database building process can be completed within 2 h, and the manual operation time is less than 30 min. It is suitable for automatic database building and compatible with capture process. All reagents in the kit underwent strict quality control and functional verification, which ensured the stability and repeatability of library preparation to the greatest extent.
Termékismertető megtekintése (angol)
VAHTS Universal Plus DNA Library Prep Kit for MGI V2 is a fragment enzyme DNA library preparation kit developed specifically for MGI high-performance sequencing platform. This kit was optimized and upgraded for the original version, which significantly reduced the proportion of Artificial Invert Chimera Reads in the FFPE sample DNA library while maintaining the original high performance, and improved the reliability of SNV detection. This kit combines DNA fragmentation, end repair and dA tailing into one step. The adapter ligation, library amplification and size selection can be performed directly without additional purification. The 100 pg – 1 μg template DNA can be converted into the library for MGI high-performance sequencing platforms. This kit is fully compatible with DNA from different sources and different input amounts. The required library can be achieved simply by adjusting the fragmentation time according to the size of the insert fragment. All the reagents provided in the kit have undergone rigorous quality control and functional testing to ensure the optimal stability and repeatability of library preparation.
Termékismertető megtekintése (angol)
VAHTS Universal Plus DNA Library Prep Kit for MGI is a DNA library preparation kit developed specifically for the MGI high-performance sequencing platform. This kit combines DNA fragmentation, end repair and dA tailing into one step. The product does not require purification, and adapter ligation, library enrichment and sorting can be performed directly. The 100 pg – 1 μg template DNA can be converted into a dedicated library for MGI high-performance sequencing platforms. Mechanical disruption of the genome is eliminated. Library preparation is simplified and the operating time is reduced. It is applicable to PCR library construction. This kit is fully compatible with DNA from different sources and different input amounts. The required library can be achieved simply by adjusting the fragmentation time according to the size of the target insert fragment. All the reagents provided in the kit have undergone rigorous quality control and functional testing to ensure the optimal stability and repeatability of library preparation.
Termékismertető megtekintése (angol)
VAHTS Universal Pro DNA Library Prep Kit for Illumina is specially designed for library preparation for next generation sequencing (NGS) on Illumina® platforms, containing FFPE DNA repair modules, suitable for library preparation from 100 pg – 1 μg of input DNA. This kit contains a DNA damage repair module that can effectively repair DNA damage caused by formalin-fixed paraffin-embedded (FFPE), such as deamination of cytosine, nicks and gaps, oxidized bases, blocked 3´ ends, etc, compatible with common DNA samples without affecting the quality of normal DNA sample libraries. Through the overall improvement of the module of end-repair, ligation and library amplification, the library conversion rate and the amplification output are greatly improved. It is widely applicable to PCR or PCR-Free library construction of multiple samples, and is compatible with targeted capture process.
