Szállítási osztály: Normál

  • Bacteria RNA Plus Reagent (R402-01)

    Bacteria RNA Plus Reagent (R402-01)

    Bacteria RNA Plus Reagent is an auxiliary reagent optimized for RNA extraction from prokaryotes (including Gram-negative and Gram-positive bacteria). It can be used with Vazyme RNA Isolater Total RNA Extraction Reagent (R401-01) to greatly improve the RNA extraction of prokaryotes. Yield, while ensuring good product quality. Bacteria RNA Plus Reagent contains an optimized buffer system, chelating agents and mild protein denaturants. When extracting bacterial RNA, a high-temperature pretreatment is added before routine operations. Together with Vazyme RNA Isolater Total RNA Extraction Reagent, it can effectively inhibit endogenous RNase promotes protein denaturation, thereby increasing the yield and quality of bacterial RNA. This reagent is also suitable for extracting RNA from yeast and other fungi.

    Vazyme oldal megtekintése (angol)

  • RNA Keeper Tissue Stabilizer (R501-01)

    RNA Keeper Tissue Stabilizer (R501-01)

    Termékismertető megtekintése (angol)

    RNA Keeper Tissue Stabilizer is a non-toxic solution that quickly penetrates into tissues, inactivates endogenous RNase, and immediately stabilizes and protects RNA integrity. Immersed in RNA Keeper Tissue Stabilizer, fresh tissue samples can be stored at 37℃ for 1 day, room temperature for 1 week, 4℃ for 4 weeks or stored at -80 ~ -20℃ for a long time, they are not required to be frozen in liquid nitrogen, and repeated Freeze-thaw does not significantly affect the integrity of the RNA. Samples stored in the RNA Keeper Tissue Stabilizer can be directly used for RNA extraction using RNA isolater total RNA extraction reagent (Vazyme #R401) or spin column (Vazyme #RC112). RNA Keeper Tissue Stabilizer can be also used for the preservation of tissues such as brain, heart, liver, pancreas, kidney, spleen, testes, muscles and the like.

  • HiScript II U+ One Step qRT-PCR Probe Kit (Q223-01)

    HiScript II U+ One Step qRT-PCR Probe Kit (Q223-01)

    Termékismertető megtekintése (angol)

    HiScript II U+ One Step qRT-PCR Probe Kit is designed for quantitative PCR with RNA (such as RNA virus) as templates. Both cDNA synthesis and PCR are performed in a single tube using gene-specific primers (GSP), without extra opening pipes and pipetting, thus it increases the quantification throughput and minimizes the chance of contamination. The kit introduces dUTP/UDG anti-contamination system. Heat-labile UDG can degrade contamination with U rapidly at room temperature and will be inactivated at 55℃ during reverse transcription, without affecting efficiency and sensitivity of RT-PCR. Integrating the advantages of HiScript II Reverse Transcriptase, Champagne Taq DNA Polymerase and optimized buffer system, One Step qRT-PCR Probe Kit enables highly sensitive detection as low as 0.1 pg of total RNA or RNA with less than 10 copies. The kit is provided as convenient Master Mix. 2×One-Step U+ Mix contains optimized buffer, dNTP/dUTP Mix, and detection system for fluorescent probes (such as TaqMan®). One-Step U+ Enzyme Mix contains proportion-optimized HiScript II Reverse Transcriptase, RNase inhibitor, Heat-labile UDG and Champagne Taq DNA Polymerase.

    Vazyme oldal megtekintése (angol)

  • HiScript II One Step qRT-PCR Probe Kit (Q222-01)

    HiScript II One Step qRT-PCR Probe Kit (Q222-01)

    Termékismertető megtekintése (angol)

    The Vazyme HiScript II One Step qRT-PCR Probe Kit is specially designed for qPCRs that directly use RNA as templates. The reverse transcription and PCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. The HiScript II Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase contained this kit enables high-sensitive total RNA detection (as little as 1 pg). The HiScript II One Step qRT-PCR Probe Kit is a master mix system. The 2× One Step Q Probe Mix contains an optimized buffer and dNTP/dUTP mix and is suitable for high-sensitive detection systems based on fluorescence labelled probes (i.e. TaqMan® ).

  • HiScript II One Step qRT-PCR SYBR Green Kit (Q221-01)

    HiScript II One Step qRT-PCR SYBR Green Kit (Q221-01)

    Termékismertető megtekintése (angol)

    HiScript Il OneStep qRT-PCR SYBR Green Kit is designed for SYBR Green I-based qPCR that directly use RNA as template. Using gene specific primers (GSP), the reverse transcription and PCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. This kit integrates the superior performance of HiScript Il Reverse Transcriptase and Champagne Taq DNA Polymerase with optimized buffer, which enables the detection sensitivity of One Step qRT-PCR SYBR Green Kit to reach 1 pg total RNA. The enhancement factor added in the buffer system can effectively reduce the formation of primer dimer and improve thes pecificity of products. This kit provides a convenient Master Mix form for easy and quick operation.

    Vazyme oldal megtekintése (angol)

  • Single Cell Sequence Specific Amplification Kit (P621-01)

    Single Cell Sequence Specific Amplification Kit (P621-01)

    Termékismertető megtekintése (angol)

    The Single Cell Squence Specific Amplification Kit is based on a one-step RT-PCR amplification method, which is used to realize the amplification of the transcriptome in single cells or trace amounts of total RNA, so that you can uncover the expression levels of different genes between individual cells. This kit realizes that RNA extraction and purification, reverse transcription and PCR reactions are completed in the same tube, no additional operations are required. It has the advantages of saving time, reducing experimental errors, reducing pollution, and improving sensitivity. This kit is also suitable for one-step expansion of 2 – 1000 cells, and the number of cycles of one-step expansion should be reduced according to the number of cells.

    Vazyme oldal megtekintése (angol)

  • HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper) (R233-01)

    HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper) (R233-01)

    Termékismertető megtekintése (angol)

    This product is based on the cost-effective HiScript ® II Reverse Transcriptase and uses uniquely designed Oligo(dT) 23 VN primers to improve the specificity and sensitivity of the reaction and obtain better quality cDNA. Different types of reverse transcription primers can be used flexibly for different experimental designs to meet diverse experimental needs. Oligo (dT) 23 VN primer/Random Hexamers or gene-specific primers (GSP) can be selected according to the needs of subsequent reverse transcription experiments. At the same time, the reaction system is greatly simplified. 5 × qRT SuperMix contains all the reagents required for reverse transcription reaction except the primers and will not freeze at -20°C, making it easy to use. The 4 × gDNA wiper Mix in the kit can completely remove residual genomic DNA contamination, ensuring that the subsequent quantitative results are more reliable. The product obtained by reverse transcription can be used in SYBR ® Green qPCR analysis method and probe analysis method for high-performance gene expression analysis.

    Vazyme oldal megtekintése (angol)

  • HiScript II Q Select RT SuperMix for qPCR (R232-01)

    HiScript II Q Select RT SuperMix for qPCR (R232-01)

    Termékismertető megtekintése (angol)

    The Vazyme HiScript II Reverse Transcriptase, optimized from M-MLV (RNase H-) Reverse Transcriptase, is a new generation reverse transcriptase with highly improved heat stability and cDNA synthesis efficiency. The HiScript II Q Select RT SuperMix for qPCR is designed for 2-step RT-qPCR. The 5× HiScript II Select qRT SuperMix contains Buffer, dNTPs, HiScript II Reverse Transcriptase, and RNase inhibitor. The Oligo (dT)23VN has a better affinity to Ploy A+ RNA than Oligo (dT)18. In addition, random hexamers and gene-specific primers (GSP) are also optional.. The HiScript II Q Select RT SuperMix for qPCR has been specially optimized for qPCR. The cDNA products are compatible for SYBR- or probe-basded qPCR, such as AceQ qPCR SYBR Green Master Mix (Vazyme, #Q111), ChamQ SYBR qPCR Master Mix (Vazyme, #Q311), ChamQ Color SYBR qPCR Master Mix (Vazyme, #Q411), and AceQ qPCR Probe Master Mix (Vazyme, #Q112).

    Vazyme oldal megtekintése (angol)

  • HiScript III RT SuperMix for qPCR (+g DNA wiper) (R323-01)

    HiScript III RT SuperMix for qPCR (+g DNA wiper) (R323-01)

    Termékismertető megtekintése (angol)

    HiScript III RT SuperMix for qPCR (+gDNA wiper) is an upgraded version of HiScript II Q RT SuperMix for qPCR (+gDNA wiper), including HiScript III Reverse Transcriptase, a new generation of reverse transcriptase with optimized Buffer. This kit further improves the efficiency of cDNA synthesis, and is suitable for two-step qRT-PCR detection. The 4 × gDNA wiper Mix in the kit can remove residual genomic DNA from the RNA template, ensuring more reliable quantitative results. It simplifies qPCR primer design without the need of spanning exon junction to design primers; 5 × HiScript III qRT SuperMix contains all components required for the reverse transcription reaction and it can be started rapidly after the addition of template RNA and RNase-free ddH2O, and the gDNA wiper is terminated to ensure the integrity of the cDNA. It is compatible with dye-based and probe-based qPCR, enabling high-performance gene expression analysis.

    Vazyme oldal megtekintése (angol)

  • HiScript II Q RT SuperMix for qPCR (R222-01)

    HiScript II Q RT SuperMix for qPCR (R222-01)

    Termékismertető megtekintése (angol)

    HiScript® II Reverse Transcriptase is a new reverse transcriptase obtained through in vitro molecular evolution technology based on M-MLV (RNase H-) Reverse Transcriptase, which greatly improves thermal stability and cDNA synthesis efficiency. HiScript® II Q RT SuperMix for qPCR is suitable for two-step qRT-PCR detection. 5 × HiScript II qRT SuperMix contains all the components required for reverse transcription reaction. The reaction can be performed quickly by adding template RNA and water. The volume of RNA template can be added up to the total volume It is very suitable for the reverse transcription reaction of low-concentration RNA template. 5 × HiScript® II qRT SuperMix will not freeze at -20°C and is easy to use.
    This product is specially optimized for qPCR. Oligo (dT)23VN has stronger anchoring ability to Poly A+ mRNA than Oligo (dT)18, making reverse transcription more efficient. The optimized ratio of Random primers/Oligo (dT)23VN primer mix enables cDNA synthesis to start from each region of the RNA transcript and has the same reverse transcription efficiency, ensuring the authenticity and repeatability of qPCR results to the greatest extent. The reverse transcription product is compatible with SYBR® Green and probe method qPCR. According to the purpose of the experiment, the corresponding reagents can be selected for use in conjunction with high-performance gene expression analysis.

    Vazyme oldal megtekintése (angol)

  • HiScipt II One Step RT-PCR Kit (Dye Plus) (P612-01)

    HiScipt II One Step RT-PCR Kit (Dye Plus) (P612-01)

    Termékismertető megtekintése (angol)

    HiScript II One Step RT-PCR Kit (Dye Plus) is specially designed for end-point PCR detection using RNA as a template (such as RNA virus). PCR products can be directly loaded for electrophoresis, which greatly saves time and labor. Using gene-specific primers (GSP), reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which improves detection throughput and reduces the risk of contamination. Integrating the superior performance of HiScript II Reverse Transcriptase and Champagne TaqTM plus DNA Polymerase, with an optimized buffer system, the detection sensitivity of HiScript II One Step RT-PCR Kit (Dye Plus) can reach 1 pg total RNA and can be amplified Fragments up to 10 kb. The kit is provided in a convenient Master Mix format. 2 × One Step Mix (Dye Plus) contains optimized buffer system, dNTP and loading dye; One Step Enzyme Mix contains optimized ratio of HiScript II Reverse Transcriptase, RNase inhibitor and Champagne TaqTM plus DNA Polymerase.

    Vazyme oldal megtekintése (angol)

  • HiScipt II One Step RT-PCR Kit (P611-01)

    HiScipt II One Step RT-PCR Kit (P611-01)

    Termékismertető megtekintése (angol)

    HiScript II One Step RT-PCR Kit is specially designed for end-point PCR detection using RNA as template (such as RNA virus). Using gene-specific primers (GSP), reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which greatly improves detection throughput and reduces the risk of contamination. Integrate HiScript II Reverse Transcriptase and the superior performance of Champagne TaqTM plus DNA Polymerase designed for long fragment amplification, and cooperate with the optimized buffer system HiScript II One Step RT-PCR Kit to amplify fragments up to 10 kb or more. The kit is provided in a convenient Master Mix format. 2 × One Step Mix contains optimized buffer system and dNTP; One Step Enzyme Mix contains optimized ratio of HiScript II Reverse Transcriptase, RNase inhibitor and Champagne TaqTM plus DNA Polymerase.

    Vazyme oldal megtekintése (angol)

  • Hiscript III Reverse Transcriptase (R302-01)

    Hiscript III Reverse Transcriptase (R302-01)

    Termékismertető megtekintése (angol)

    HiScript III Reverse Transcriptase is an upgraded version of HiScript II Reverse Transcriptase, which can perform highly efficient reverse transcription reactions at 37°C. HiScript III Reverse Transcriptase still retains the thermal stability of the second-generation product HiScript II Reverse Transcriptase. For RNA with complex secondary structure, the reverse transcription temperature can be increased To 50~55℃, avoid the inhibition of cDNA synthesis by RNA complex secondary structure, and can effectively synthesize high-quality cDNA. In addition, this product still has superior continuous synthesis ability and super Strong impurity tolerance.

    Vazyme oldal megtekintése (angol)

  • M-MLV(H-) Reverse Transcriptase (R021-01)

    M-MLV(H-) Reverse Transcriptase (R021-01)

    Termékismertető megtekintése (angol)

    The wildtype Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase has the following activities: RNA-dependent DNA polymerase, DNA-dependent DNA polymerase, and RNase H. The M-MLV (H-) Reverse Transcriptase is a single-site mutant of M-MLV which contains no RNase H activity. Compared with M-MLV mutants obtained via deletion of the RNase H domian, this product, which retains a complete protein structure and polymerase activities, can be used for the synthesis of longer cDNA or the preparation of cDNA library.

  • T4 Ligase (C301-01)

    T4 Ligase (C301-01)

    Termékismertető megtekintése (angol)

    The T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the adjacent 5′-phosphate and 3′-hydroxyl on the blunt or cohesive end of dsDNA. It can also catalyze the linkage of RNA with ssDNA or RNA in double stranded nucleic acids. However, it cannot catalyze linkages between single stranded nucleotides. The T4 DNA Ligase can be used in labelling the 3′-end of RNA, cyclizing RNA and DNA oligonucleotides, cloning of cDNA, and other manipulation of nucleic acids.

  • dGTP (100 mM) (P037-01)

    dGTP (100 mM) (P037-01)

    Coming soon…

  • dCTP (100 mM) (P036-01)

    dCTP (100 mM) (P036-01)

    Coming soon…

  • dTTP (100 mM) (P035-01)

    dTTP (100 mM) (P035-01)

    Coming soon…

  • dATP (100 mM) (P034-01)

    dATP (100 mM) (P034-01)

    Coming soon…

  • dUTP (100 mM) (P033-01)

    dUTP (100 mM) (P033-01)

    Coming soon…